Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaS and ybaT are separated by 2 nucleotides ybaT and cueR are separated by 124 nucleotides cueR and ybbJ are separated by 0 nucleotides ybbJ and ybbK overlap by 4 nucleotides
b0485: ybaS - predicted glutaminase (NCBI), at 510,865 to 511,797
ybaS
b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092
ybaT
b0487: cueR - DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI), at 513,217 to 513,624
cueR
b0488: ybbJ - conserved inner membrane protein (RefSeq), at 513,625 to 514,083
ybbJ
b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997
ybbK
Position (kb) 511
512
513
514 Strain fitness (log2 ratio) -2
-1
0
1
2 at 510.844 kb on + strand at 510.903 kb on + strand at 510.903 kb on + strand at 510.979 kb on - strand, within ybaS at 510.979 kb on - strand, within ybaS at 511.000 kb on - strand, within ybaS at 511.172 kb on + strand, within ybaS at 511.172 kb on + strand, within ybaS at 511.359 kb on + strand, within ybaS at 511.389 kb on - strand, within ybaS at 511.392 kb on - strand, within ybaS at 511.434 kb on + strand, within ybaS at 511.583 kb on + strand, within ybaS at 511.583 kb on + strand, within ybaS at 511.673 kb on + strand, within ybaS at 511.673 kb on + strand, within ybaS at 511.673 kb on + strand, within ybaS at 511.980 kb on + strand, within ybaT at 512.081 kb on + strand, within ybaT at 512.081 kb on + strand, within ybaT at 512.171 kb on + strand, within ybaT at 512.239 kb on - strand, within ybaT at 512.413 kb on - strand, within ybaT at 512.414 kb on - strand, within ybaT at 512.514 kb on - strand, within ybaT at 512.567 kb on - strand, within ybaT at 512.604 kb on + strand, within ybaT at 512.771 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.846 kb on - strand, within ybaT at 512.886 kb on + strand, within ybaT at 512.890 kb on + strand, within ybaT at 512.953 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.991 kb on + strand at 512.998 kb on + strand at 512.998 kb on + strand at 513.013 kb on - strand at 513.013 kb on - strand at 513.039 kb on + strand at 513.039 kb on + strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.077 kb on + strand at 513.077 kb on + strand at 513.095 kb on - strand at 513.107 kb on + strand at 513.109 kb on + strand at 513.182 kb on + strand at 513.205 kb on - strand at 513.287 kb on - strand, within cueR at 513.295 kb on - strand, within cueR at 513.431 kb on - strand, within cueR at 513.480 kb on + strand, within cueR at 513.537 kb on - strand, within cueR at 513.537 kb on - strand, within cueR at 513.700 kb on - strand, within ybbJ at 513.728 kb on - strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.977 kb on + strand, within ybbJ
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 510,844 + -0.0 510,903 + +0.9 510,903 + -0.6 510,979 - ybaS b0485 0.12 -0.1 510,979 - ybaS b0485 0.12 +0.3 511,000 - ybaS b0485 0.14 -0.5 511,172 + ybaS b0485 0.33 +0.3 511,172 + ybaS b0485 0.33 +0.8 511,359 + ybaS b0485 0.53 +1.3 511,389 - ybaS b0485 0.56 +1.0 511,392 - ybaS b0485 0.56 +0.5 511,434 + ybaS b0485 0.61 -0.1 511,583 + ybaS b0485 0.77 +0.8 511,583 + ybaS b0485 0.77 -0.3 511,673 + ybaS b0485 0.87 +0.4 511,673 + ybaS b0485 0.87 -0.1 511,673 + ybaS b0485 0.87 -0.5 511,980 + ybaT b0486 0.14 +0.3 512,081 + ybaT b0486 0.22 -0.5 512,081 + ybaT b0486 0.22 -0.3 512,171 + ybaT b0486 0.29 +0.3 512,239 - ybaT b0486 0.34 -0.1 512,413 - ybaT b0486 0.47 -0.3 512,414 - ybaT b0486 0.47 -0.5 512,514 - ybaT b0486 0.55 -0.9 512,567 - ybaT b0486 0.59 -1.9 512,604 + ybaT b0486 0.62 -0.0 512,771 + ybaT b0486 0.75 -0.3 512,830 + ybaT b0486 0.80 -0.7 512,830 + ybaT b0486 0.80 -1.7 512,846 - ybaT b0486 0.81 -1.7 512,886 + ybaT b0486 0.84 -1.3 512,890 + ybaT b0486 0.84 -0.0 512,953 + ybaT b0486 0.89 +0.9 512,958 + ybaT b0486 0.90 +0.9 512,958 + ybaT b0486 0.90 +1.3 512,991 + -0.5 512,998 + -0.1 512,998 + -1.1 513,013 - +0.5 513,013 - -0.5 513,039 + +1.9 513,039 + -0.7 513,057 - -1.0 513,057 - -0.3 513,057 - -2.0 513,077 + -0.7 513,077 + +0.7 513,095 - -1.1 513,107 + -1.9 513,109 + -0.3 513,182 + -0.4 513,205 - -0.5 513,287 - cueR b0487 0.17 -0.3 513,295 - cueR b0487 0.19 +0.4 513,431 - cueR b0487 0.52 -0.8 513,480 + cueR b0487 0.64 -0.5 513,537 - cueR b0487 0.78 +0.3 513,537 - cueR b0487 0.78 +0.3 513,700 - ybbJ b0488 0.16 -0.0 513,728 - ybbJ b0488 0.22 -0.1 513,742 + ybbJ b0488 0.25 +1.0 513,742 + ybbJ b0488 0.25 -2.0 513,949 - ybbJ b0488 0.71 +0.3 513,949 - ybbJ b0488 0.71 -0.7 513,958 + ybbJ b0488 0.73 -0.5 513,958 + ybbJ b0488 0.73 +1.1 513,977 + ybbJ b0488 0.77 +0.6
Or see this region's nucleotide sequence