Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaX and ybaB are separated by 52 nucleotides ybaB and recR overlap by 1 nucleotides recR and htpG are separated by 109 nucleotides
b0470: dnaX - DNA polymerase III subunits gamma and tau (NCBI), at 491,316 to 493,247
dnaX
b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629
ybaB
b0472: recR - recombination protein RecR (NCBI), at 493,629 to 494,234
recR
b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218
htpG
Position (kb)
493
494 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 493.296 kb on + strand at 493.296 kb on + strand at 493.320 kb on + strand at 493.327 kb on - strand at 493.392 kb on + strand, within ybaB at 493.488 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.499 kb on - strand, within ybaB at 493.517 kb on + strand, within ybaB at 493.713 kb on - strand, within recR at 493.724 kb on + strand, within recR at 493.879 kb on + strand, within recR at 493.882 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.887 kb on - strand, within recR at 493.949 kb on + strand, within recR at 493.993 kb on + strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.016 kb on - strand, within recR at 494.018 kb on + strand, within recR at 494.057 kb on + strand, within recR at 494.074 kb on + strand, within recR at 494.094 kb on - strand, within recR at 494.099 kb on - strand, within recR at 494.115 kb on + strand, within recR at 494.211 kb on - strand at 494.211 kb on - strand at 494.211 kb on - strand at 494.291 kb on - strand at 494.345 kb on - strand at 494.345 kb on - strand at 494.448 kb on + strand at 494.517 kb on - strand at 494.561 kb on + strand, within htpG at 494.596 kb on - strand, within htpG at 494.619 kb on - strand, within htpG
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 493,296 + +0.9 493,296 + +1.3 493,320 + +0.3 493,327 - -0.5 493,392 + ybaB b0471 0.28 +0.5 493,488 - ybaB b0471 0.57 -0.7 493,499 - ybaB b0471 0.60 -0.7 493,499 - ybaB b0471 0.60 -0.9 493,517 + ybaB b0471 0.66 -2.3 493,713 - recR b0472 0.14 -1.7 493,724 + recR b0472 0.16 +1.3 493,879 + recR b0472 0.41 +0.9 493,882 - recR b0472 0.42 -0.7 493,887 - recR b0472 0.43 -0.5 493,887 - recR b0472 0.43 -0.7 493,949 + recR b0472 0.53 -1.5 493,993 + recR b0472 0.60 +1.5 494,016 - recR b0472 0.64 +0.3 494,016 - recR b0472 0.64 -0.0 494,016 - recR b0472 0.64 +1.9 494,018 + recR b0472 0.64 -0.3 494,057 + recR b0472 0.71 +0.9 494,074 + recR b0472 0.73 +0.3 494,094 - recR b0472 0.77 -0.1 494,099 - recR b0472 0.78 -0.5 494,115 + recR b0472 0.80 +0.3 494,211 - -0.2 494,211 - -1.7 494,211 - -0.7 494,291 - -1.7 494,345 - +0.3 494,345 - +0.3 494,448 + +0.3 494,517 - -0.6 494,561 + htpG b0473 0.12 +0.3 494,596 - htpG b0473 0.13 -0.3 494,619 - htpG b0473 0.15 -1.7
Or see this region's nucleotide sequence