Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahD and yahE are separated by 39 nucleotides yahE and yahF overlap by 11 nucleotides yahF and yahG overlap by 1 nucleotides
b0318: yahD - predicted transcriptional regulator with ankyrin domain (NCBI), at 334,504 to 335,109
yahD
b0319: yahE - hypothetical protein (NCBI), at 335,149 to 336,012
yahE
b0320: yahF - predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain (NCBI), at 336,002 to 337,549
yahF
b0321: yahG - hypothetical protein (NCBI), at 337,549 to 338,967
yahG
Position (kb) 336
337
338 Strain fitness (log2 ratio) -2
-1
0
1
2 at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.009 kb on + strand, within yahD at 335.017 kb on - strand, within yahD at 335.017 kb on - strand, within yahD at 335.058 kb on + strand at 335.058 kb on + strand at 335.088 kb on + strand at 335.089 kb on - strand at 335.118 kb on - strand at 335.122 kb on + strand at 335.122 kb on + strand at 335.181 kb on + strand at 335.214 kb on + strand at 335.214 kb on + strand at 335.278 kb on - strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.280 kb on + strand, within yahE at 335.286 kb on - strand, within yahE at 335.324 kb on + strand, within yahE at 335.324 kb on - strand, within yahE at 335.343 kb on + strand, within yahE at 335.357 kb on + strand, within yahE at 335.372 kb on + strand, within yahE at 335.372 kb on + strand, within yahE at 335.391 kb on + strand, within yahE at 335.391 kb on + strand, within yahE at 335.396 kb on + strand, within yahE at 335.417 kb on + strand, within yahE at 335.417 kb on + strand, within yahE at 335.418 kb on + strand, within yahE at 335.418 kb on + strand, within yahE at 335.429 kb on + strand, within yahE at 335.429 kb on + strand, within yahE at 335.469 kb on + strand, within yahE at 335.473 kb on + strand, within yahE at 335.473 kb on + strand, within yahE at 335.474 kb on + strand, within yahE at 335.479 kb on + strand, within yahE at 335.482 kb on + strand, within yahE at 335.494 kb on + strand, within yahE at 335.516 kb on + strand, within yahE at 335.653 kb on + strand, within yahE at 335.654 kb on + strand, within yahE at 335.654 kb on + strand, within yahE at 335.655 kb on + strand, within yahE at 335.655 kb on + strand, within yahE at 335.657 kb on - strand, within yahE at 335.661 kb on - strand, within yahE at 335.666 kb on + strand, within yahE at 335.673 kb on + strand, within yahE at 335.817 kb on - strand, within yahE at 335.817 kb on - strand, within yahE at 335.883 kb on + strand, within yahE at 335.883 kb on + strand, within yahE at 335.899 kb on - strand, within yahE at 335.899 kb on - strand, within yahE at 335.904 kb on - strand, within yahE at 335.944 kb on + strand at 335.944 kb on + strand at 335.949 kb on + strand at 335.950 kb on + strand at 335.950 kb on + strand at 335.950 kb on + strand at 335.952 kb on + strand at 335.952 kb on + strand at 335.957 kb on - strand at 335.957 kb on - strand at 335.958 kb on - strand at 335.958 kb on - strand at 335.958 kb on - strand at 335.960 kb on - strand at 335.960 kb on - strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.969 kb on + strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.970 kb on - strand at 335.974 kb on + strand at 335.974 kb on + strand at 335.974 kb on - strand at 335.977 kb on - strand at 335.977 kb on - strand at 335.977 kb on - strand at 335.986 kb on + strand at 335.988 kb on - strand at 335.995 kb on - strand at 335.995 kb on - strand at 336.005 kb on - strand at 336.005 kb on - strand at 336.084 kb on - strand at 336.218 kb on - strand, within yahF at 336.218 kb on - strand, within yahF at 336.227 kb on + strand, within yahF at 336.236 kb on + strand, within yahF at 336.238 kb on + strand, within yahF at 336.252 kb on + strand, within yahF at 336.262 kb on + strand, within yahF at 336.262 kb on + strand, within yahF at 336.294 kb on + strand, within yahF at 336.306 kb on - strand, within yahF at 336.306 kb on - strand, within yahF at 336.308 kb on + strand, within yahF at 336.368 kb on + strand, within yahF at 336.368 kb on + strand, within yahF at 336.554 kb on - strand, within yahF at 337.024 kb on - strand, within yahF at 337.163 kb on - strand, within yahF at 337.169 kb on + strand, within yahF at 337.169 kb on + strand, within yahF at 337.236 kb on - strand, within yahF at 337.603 kb on + strand at 337.603 kb on + strand at 337.763 kb on + strand, within yahG at 337.763 kb on + strand, within yahG at 337.821 kb on + strand, within yahG at 337.829 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 338.157 kb on + strand, within yahG at 338.157 kb on + strand, within yahG at 338.178 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.188 kb on + strand, within yahG at 338.196 kb on - strand, within yahG at 338.196 kb on - strand, within yahG at 338.231 kb on + strand, within yahG at 338.237 kb on - strand, within yahG at 338.266 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.424 kb on - strand, within yahG at 338.429 kb on - strand, within yahG
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 335,009 + yahD b0318 0.83 +1.1 335,009 + yahD b0318 0.83 -0.2 335,009 + yahD b0318 0.83 +0.8 335,009 + yahD b0318 0.83 -0.2 335,017 - yahD b0318 0.85 -0.5 335,017 - yahD b0318 0.85 +0.3 335,058 + -0.9 335,058 + -0.8 335,088 + -0.7 335,089 - -0.7 335,118 - +2.1 335,122 + +1.3 335,122 + -2.1 335,181 + +0.1 335,214 + -0.2 335,214 + +0.7 335,278 - yahE b0319 0.15 +0.3 335,280 + yahE b0319 0.15 +0.3 335,280 + yahE b0319 0.15 +0.3 335,280 + yahE b0319 0.15 +0.8 335,280 + yahE b0319 0.15 -0.2 335,286 - yahE b0319 0.16 -0.4 335,324 + yahE b0319 0.20 -2.0 335,324 - yahE b0319 0.20 +0.1 335,343 + yahE b0319 0.22 -1.2 335,357 + yahE b0319 0.24 -0.5 335,372 + yahE b0319 0.26 -1.5 335,372 + yahE b0319 0.26 -0.0 335,391 + yahE b0319 0.28 -0.7 335,391 + yahE b0319 0.28 +0.3 335,396 + yahE b0319 0.29 +0.9 335,417 + yahE b0319 0.31 -0.5 335,417 + yahE b0319 0.31 +0.8 335,418 + yahE b0319 0.31 -0.5 335,418 + yahE b0319 0.31 -1.1 335,429 + yahE b0319 0.32 +0.9 335,429 + yahE b0319 0.32 -1.2 335,469 + yahE b0319 0.37 -0.7 335,473 + yahE b0319 0.38 -0.9 335,473 + yahE b0319 0.38 +1.3 335,474 + yahE b0319 0.38 -0.0 335,479 + yahE b0319 0.38 +0.7 335,482 + yahE b0319 0.39 -0.0 335,494 + yahE b0319 0.40 -1.2 335,516 + yahE b0319 0.42 -0.7 335,653 + yahE b0319 0.58 -0.3 335,654 + yahE b0319 0.58 +0.3 335,654 + yahE b0319 0.58 -0.1 335,655 + yahE b0319 0.59 +0.3 335,655 + yahE b0319 0.59 +0.0 335,657 - yahE b0319 0.59 +0.9 335,661 - yahE b0319 0.59 -0.2 335,666 + yahE b0319 0.60 +0.9 335,673 + yahE b0319 0.61 -0.2 335,817 - yahE b0319 0.77 -0.9 335,817 - yahE b0319 0.77 +0.3 335,883 + yahE b0319 0.85 +0.7 335,883 + yahE b0319 0.85 -1.2 335,899 - yahE b0319 0.87 +0.1 335,899 - yahE b0319 0.87 +0.8 335,904 - yahE b0319 0.87 +0.9 335,944 + +0.3 335,944 + -0.6 335,949 + -0.2 335,950 + +0.6 335,950 + +0.5 335,950 + +0.0 335,952 + +0.1 335,952 + +0.0 335,957 - -1.7 335,957 - +0.9 335,958 - -0.4 335,958 - +0.3 335,958 - -0.3 335,960 - +0.6 335,960 - -1.7 335,969 + -1.0 335,969 + -0.9 335,969 + -1.2 335,969 + +1.7 335,969 + +1.3 335,969 + +2.0 335,969 + +0.0 335,969 + +0.9 335,970 - -0.1 335,970 - -0.2 335,970 - -0.5 335,970 - -0.7 335,970 - -0.1 335,970 - -2.0 335,974 + -0.7 335,974 + +0.1 335,974 - -0.7 335,977 - -0.1 335,977 - -1.5 335,977 - +0.9 335,986 + -2.0 335,988 - +0.3 335,995 - -0.3 335,995 - -1.0 336,005 - -0.2 336,005 - +0.1 336,084 - -0.1 336,218 - yahF b0320 0.14 +0.7 336,218 - yahF b0320 0.14 +1.8 336,227 + yahF b0320 0.15 +0.8 336,236 + yahF b0320 0.15 -0.9 336,238 + yahF b0320 0.15 -0.0 336,252 + yahF b0320 0.16 +0.1 336,262 + yahF b0320 0.17 +0.7 336,262 + yahF b0320 0.17 +1.1 336,294 + yahF b0320 0.19 +1.3 336,306 - yahF b0320 0.20 -0.4 336,306 - yahF b0320 0.20 -0.7 336,308 + yahF b0320 0.20 -0.1 336,368 + yahF b0320 0.24 -1.0 336,368 + yahF b0320 0.24 -0.5 336,554 - yahF b0320 0.36 -0.7 337,024 - yahF b0320 0.66 -0.1 337,163 - yahF b0320 0.75 +0.7 337,169 + yahF b0320 0.75 +1.1 337,169 + yahF b0320 0.75 +1.1 337,236 - yahF b0320 0.80 +1.5 337,603 + -0.1 337,603 + +2.7 337,763 + yahG b0321 0.15 -0.8 337,763 + yahG b0321 0.15 +1.9 337,821 + yahG b0321 0.19 -0.1 337,829 - yahG b0321 0.20 +0.6 337,974 - yahG b0321 0.30 +1.3 337,974 - yahG b0321 0.30 +0.3 338,157 + yahG b0321 0.43 +0.6 338,157 + yahG b0321 0.43 +0.7 338,178 + yahG b0321 0.44 -1.4 338,183 + yahG b0321 0.45 -0.1 338,183 + yahG b0321 0.45 -0.9 338,188 + yahG b0321 0.45 +0.3 338,196 - yahG b0321 0.46 +0.7 338,196 - yahG b0321 0.46 +0.3 338,231 + yahG b0321 0.48 +0.1 338,237 - yahG b0321 0.48 +0.3 338,266 + yahG b0321 0.51 +0.0 338,365 + yahG b0321 0.58 +0.7 338,365 + yahG b0321 0.58 +0.9 338,424 - yahG b0321 0.62 +0.3 338,429 - yahG b0321 0.62 +0.4
Or see this region's nucleotide sequence