Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt betB and betI are separated by 13 nucleotides betI and betT are separated by 128 nucleotides
b0312: betB - betaine aldehyde dehydrogenase, NAD-dependent (NCBI), at 326,485 to 327,957
betB
b0313: betI - probably transcriptional repressor of bet genes (VIMSS), at 327,971 to 328,558
betI
b0314: betT - choline transporter of high affinity (NCBI), at 328,687 to 330,720
betT
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 327.090 kb on + strand, within betB at 327.097 kb on + strand, within betB at 327.097 kb on + strand, within betB at 327.101 kb on - strand, within betB at 327.413 kb on - strand, within betB at 327.422 kb on - strand, within betB at 327.447 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.544 kb on + strand, within betB at 327.584 kb on + strand, within betB at 327.606 kb on - strand, within betB at 327.723 kb on + strand, within betB at 327.723 kb on + strand, within betB at 327.870 kb on - strand at 327.870 kb on - strand at 327.910 kb on + strand at 327.910 kb on + strand at 328.001 kb on - strand at 328.009 kb on - strand at 328.054 kb on - strand, within betI at 328.104 kb on + strand, within betI at 328.104 kb on + strand, within betI at 328.188 kb on + strand, within betI at 328.213 kb on - strand, within betI at 328.213 kb on - strand, within betI at 328.220 kb on - strand, within betI at 328.303 kb on + strand, within betI at 328.303 kb on + strand, within betI at 328.360 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.483 kb on - strand, within betI at 328.484 kb on - strand, within betI at 328.526 kb on + strand at 328.526 kb on + strand at 328.566 kb on + strand at 328.628 kb on - strand at 328.628 kb on - strand at 328.758 kb on + strand at 329.044 kb on + strand, within betT at 329.044 kb on + strand, within betT at 329.054 kb on - strand, within betT at 329.054 kb on - strand, within betT at 329.061 kb on + strand, within betT at 329.111 kb on - strand, within betT at 329.111 kb on - strand, within betT at 329.111 kb on - strand, within betT at 329.113 kb on + strand, within betT at 329.138 kb on + strand, within betT at 329.212 kb on - strand, within betT at 329.250 kb on + strand, within betT at 329.270 kb on + strand, within betT at 329.276 kb on - strand, within betT at 329.293 kb on + strand, within betT at 329.313 kb on + strand, within betT at 329.366 kb on - strand, within betT at 329.402 kb on - strand, within betT at 329.403 kb on - strand, within betT at 329.443 kb on - strand, within betT
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 327,090 + betB b0312 0.41 +0.3 327,097 + betB b0312 0.42 -1.2 327,097 + betB b0312 0.42 +1.3 327,101 - betB b0312 0.42 +0.3 327,413 - betB b0312 0.63 -1.7 327,422 - betB b0312 0.64 -0.2 327,447 + betB b0312 0.65 +0.3 327,480 + betB b0312 0.68 +0.9 327,480 + betB b0312 0.68 +0.3 327,480 + betB b0312 0.68 +2.1 327,544 + betB b0312 0.72 +0.6 327,584 + betB b0312 0.75 +1.0 327,606 - betB b0312 0.76 +0.8 327,723 + betB b0312 0.84 +2.9 327,723 + betB b0312 0.84 -2.0 327,870 - -0.7 327,870 - +0.3 327,910 + +0.3 327,910 + -0.7 328,001 - +1.5 328,009 - -0.7 328,054 - betI b0313 0.14 -0.7 328,104 + betI b0313 0.23 -2.0 328,104 + betI b0313 0.23 -0.2 328,188 + betI b0313 0.37 -0.1 328,213 - betI b0313 0.41 -0.8 328,213 - betI b0313 0.41 +0.9 328,220 - betI b0313 0.42 -1.0 328,303 + betI b0313 0.56 -1.0 328,303 + betI b0313 0.56 +1.3 328,360 + betI b0313 0.66 -1.5 328,475 + betI b0313 0.86 +0.6 328,475 + betI b0313 0.86 +0.3 328,483 - betI b0313 0.87 -0.8 328,484 - betI b0313 0.87 +0.3 328,526 + -0.2 328,526 + +1.1 328,566 + +0.9 328,628 - +0.2 328,628 - -0.3 328,758 + -0.1 329,044 + betT b0314 0.18 +0.7 329,044 + betT b0314 0.18 -1.2 329,054 - betT b0314 0.18 +2.3 329,054 - betT b0314 0.18 -0.2 329,061 + betT b0314 0.18 -1.5 329,111 - betT b0314 0.21 -0.7 329,111 - betT b0314 0.21 +0.9 329,111 - betT b0314 0.21 -0.2 329,113 + betT b0314 0.21 -0.3 329,138 + betT b0314 0.22 +0.0 329,212 - betT b0314 0.26 -0.2 329,250 + betT b0314 0.28 +0.0 329,270 + betT b0314 0.29 +0.3 329,276 - betT b0314 0.29 +0.1 329,293 + betT b0314 0.30 -1.0 329,313 + betT b0314 0.31 -0.4 329,366 - betT b0314 0.33 +1.1 329,402 - betT b0314 0.35 -0.0 329,403 - betT b0314 0.35 +0.0 329,443 - betT b0314 0.37 -0.7
Or see this region's nucleotide sequence