Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-1 and ykfD are separated by 207 nucleotides ykfD and mmuM overlap by 14 nucleotides
b0259: insH-1 - CP4-6 prophage; IS5 transposase and trans-activator (NCBI), at 273,325 to 274,341
insH-1
b0260: ykfD - putative amino acid/amine transport protein (VIMSS), at 274,549 to 275,952
ykfD
b0261: mmuM - homocysteine methyltransferase (NCBI), at 275,939 to 276,871
mmuM
Position (kb) 274
275
276 Strain fitness (log2 ratio) -1
0
1
2 at 274.371 kb on + strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.428 kb on + strand at 274.461 kb on + strand at 274.497 kb on + strand at 274.712 kb on + strand, within ykfD at 274.725 kb on - strand, within ykfD at 274.725 kb on - strand, within ykfD at 274.781 kb on - strand, within ykfD at 274.781 kb on - strand, within ykfD at 274.790 kb on + strand, within ykfD at 274.791 kb on - strand, within ykfD at 274.851 kb on + strand, within ykfD at 274.855 kb on - strand, within ykfD at 274.908 kb on - strand, within ykfD at 274.913 kb on - strand, within ykfD at 274.932 kb on + strand, within ykfD at 274.950 kb on - strand, within ykfD at 274.950 kb on - strand, within ykfD at 274.955 kb on - strand, within ykfD at 275.190 kb on - strand, within ykfD at 275.516 kb on - strand, within ykfD at 275.585 kb on - strand, within ykfD at 275.627 kb on - strand, within ykfD at 275.883 kb on + strand at 275.883 kb on + strand at 275.976 kb on - strand at 275.986 kb on - strand at 275.988 kb on - strand at 275.988 kb on - strand at 276.000 kb on - strand at 276.059 kb on + strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.333 kb on + strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.358 kb on + strand, within mmuM at 276.415 kb on + strand, within mmuM at 276.580 kb on - strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.669 kb on + strand, within mmuM at 276.677 kb on - strand, within mmuM at 276.682 kb on + strand, within mmuM at 276.685 kb on + strand, within mmuM at 276.882 kb on + strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 274,371 + -1.2 274,376 - -1.2 274,376 - +0.4 274,428 + +0.4 274,461 + -0.6 274,497 + -1.2 274,712 + ykfD b0260 0.12 -0.0 274,725 - ykfD b0260 0.13 +1.4 274,725 - ykfD b0260 0.13 +1.9 274,781 - ykfD b0260 0.17 +1.1 274,781 - ykfD b0260 0.17 -0.4 274,790 + ykfD b0260 0.17 +1.4 274,791 - ykfD b0260 0.17 +0.9 274,851 + ykfD b0260 0.22 +1.0 274,855 - ykfD b0260 0.22 +0.6 274,908 - ykfD b0260 0.26 +1.6 274,913 - ykfD b0260 0.26 +0.4 274,932 + ykfD b0260 0.27 +0.8 274,950 - ykfD b0260 0.29 +1.9 274,950 - ykfD b0260 0.29 +0.4 274,955 - ykfD b0260 0.29 +0.4 275,190 - ykfD b0260 0.46 -0.2 275,516 - ykfD b0260 0.69 +0.4 275,585 - ykfD b0260 0.74 +0.5 275,627 - ykfD b0260 0.77 +0.2 275,883 + -0.9 275,883 + +0.8 275,976 - +0.8 275,986 - +1.7 275,988 - -1.4 275,988 - +0.4 276,000 - +0.1 276,059 + mmuM b0261 0.13 +0.4 276,073 - mmuM b0261 0.14 +0.7 276,073 - mmuM b0261 0.14 -0.6 276,333 + mmuM b0261 0.42 -0.6 276,346 - mmuM b0261 0.44 +0.1 276,346 - mmuM b0261 0.44 -0.6 276,358 + mmuM b0261 0.45 -0.7 276,415 + mmuM b0261 0.51 -0.2 276,580 - mmuM b0261 0.69 -0.2 276,621 + mmuM b0261 0.73 +0.4 276,621 + mmuM b0261 0.73 +0.1 276,669 + mmuM b0261 0.78 -1.2 276,677 - mmuM b0261 0.79 +0.4 276,682 + mmuM b0261 0.80 -0.5 276,685 + mmuM b0261 0.80 +0.6 276,882 + -0.2 276,890 - +1.4 276,890 - -0.2 276,890 - +0.9 276,890 - +1.9 276,931 + +0.4
Or see this region's nucleotide sequence