Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadC and ampD are separated by 87 nucleotides ampD and ampE overlap by 4 nucleotides ampE and aroP are separated by 42 nucleotides
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 118.107 kb on - strand, within nadC at 118.201 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.296 kb on + strand, within nadC at 118.355 kb on - strand, within nadC at 118.355 kb on - strand, within nadC at 118.364 kb on + strand, within nadC at 118.382 kb on - strand, within nadC at 118.538 kb on + strand, within nadC at 118.623 kb on + strand at 118.631 kb on - strand at 118.737 kb on + strand at 118.737 kb on + strand at 118.781 kb on + strand at 118.836 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.904 kb on - strand, within ampD at 119.033 kb on - strand, within ampD at 119.101 kb on + strand, within ampD at 119.109 kb on - strand, within ampD at 119.109 kb on - strand, within ampD at 119.117 kb on - strand, within ampD at 119.119 kb on + strand, within ampD at 119.150 kb on - strand, within ampD at 119.150 kb on - strand, within ampD at 119.163 kb on + strand, within ampD at 119.168 kb on - strand, within ampD at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE at 119.753 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.756 kb on + strand, within ampE at 119.772 kb on - strand, within ampE at 119.786 kb on + strand, within ampE at 119.786 kb on + strand, within ampE at 119.799 kb on - strand, within ampE at 119.799 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.915 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.983 kb on + strand, within ampE at 119.985 kb on - strand, within ampE at 120.006 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.037 kb on - strand, within ampE at 120.138 kb on - strand at 120.139 kb on + strand at 120.153 kb on - strand at 120.155 kb on + strand at 120.237 kb on - strand at 120.251 kb on - strand at 120.254 kb on + strand at 120.266 kb on - strand at 120.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 117,839 + +0.4 117,856 - nadC b0109 0.12 -0.3 117,856 - nadC b0109 0.12 +2.0 117,879 - nadC b0109 0.14 -0.6 117,879 - nadC b0109 0.14 -0.9 117,881 - nadC b0109 0.14 +0.4 117,922 - nadC b0109 0.19 +0.4 117,922 - nadC b0109 0.19 +1.4 118,107 - nadC b0109 0.40 +0.5 118,201 + nadC b0109 0.50 +0.8 118,230 + nadC b0109 0.53 -0.6 118,230 + nadC b0109 0.53 +2.7 118,296 + nadC b0109 0.61 +0.2 118,355 - nadC b0109 0.67 +0.1 118,355 - nadC b0109 0.67 +1.4 118,364 + nadC b0109 0.68 +0.4 118,382 - nadC b0109 0.70 -1.2 118,538 + nadC b0109 0.88 +0.4 118,623 + +0.9 118,631 - -0.1 118,737 + +0.5 118,737 + +0.4 118,781 + -0.6 118,836 + ampD b0110 0.19 +1.1 118,868 + ampD b0110 0.24 +2.4 118,868 + ampD b0110 0.24 -1.6 118,904 - ampD b0110 0.31 +0.1 119,033 - ampD b0110 0.54 -1.1 119,101 + ampD b0110 0.67 +1.3 119,109 - ampD b0110 0.68 -0.2 119,109 - ampD b0110 0.68 +0.2 119,117 - ampD b0110 0.70 -0.8 119,119 + ampD b0110 0.70 +0.8 119,150 - ampD b0110 0.76 -0.3 119,150 - ampD b0110 0.76 +0.9 119,163 + ampD b0110 0.78 +1.3 119,168 - ampD b0110 0.79 +2.4 119,191 + ampD b0110 0.83 +3.9 119,191 + ampD b0110 0.83 +1.1 119,274 + +0.7 119,339 + -0.0 119,339 + -1.6 119,499 - ampE b0111 0.25 +0.1 119,499 - ampE b0111 0.25 +0.4 119,522 + ampE b0111 0.28 -1.6 119,528 - ampE b0111 0.29 +0.5 119,555 - ampE b0111 0.32 -1.9 119,555 - ampE b0111 0.32 +0.4 119,558 - ampE b0111 0.32 -1.2 119,567 + ampE b0111 0.33 +1.0 119,567 + ampE b0111 0.33 -0.2 119,753 - ampE b0111 0.55 +0.1 119,755 - ampE b0111 0.55 +0.4 119,755 - ampE b0111 0.55 +0.1 119,756 + ampE b0111 0.56 +0.3 119,772 - ampE b0111 0.57 -0.8 119,786 + ampE b0111 0.59 -1.6 119,786 + ampE b0111 0.59 +0.3 119,799 - ampE b0111 0.61 -0.6 119,799 - ampE b0111 0.61 -0.1 119,836 - ampE b0111 0.65 +1.6 119,836 - ampE b0111 0.65 -0.7 119,915 + ampE b0111 0.74 +0.4 119,961 + ampE b0111 0.80 -0.6 119,961 + ampE b0111 0.80 +0.4 119,983 + ampE b0111 0.82 -2.2 119,985 - ampE b0111 0.82 -0.0 120,006 - ampE b0111 0.85 -0.5 120,013 - ampE b0111 0.86 +1.4 120,013 - ampE b0111 0.86 +3.6 120,014 + ampE b0111 0.86 -0.6 120,014 + ampE b0111 0.86 +0.6 120,014 + ampE b0111 0.86 +1.6 120,014 + ampE b0111 0.86 -1.2 120,037 - ampE b0111 0.88 +0.4 120,138 - -0.3 120,139 + -0.1 120,153 - +0.4 120,155 + -0.9 120,237 - +0.0 120,251 - +0.9 120,254 + -1.2 120,266 - +0.4 120,266 - -1.6
Or see this region's nucleotide sequence