Strain Fitness in Pseudomonas sp. RS175 around PFR28_04422

Experiment: Adenosine (C) 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFR28_04421 and PFR28_04422 are separated by 12 nucleotidesPFR28_04422 and PFR28_04423 are separated by 167 nucleotidesPFR28_04423 and PFR28_04424 are separated by 57 nucleotides PFR28_04421: PFR28_04421 - hypothetical protein, at 5,034,318 to 5,035,181 _04421 PFR28_04422: PFR28_04422 - Guanylate kinase, at 5,035,194 to 5,035,814 _04422 PFR28_04423: PFR28_04423 - DNA-directed RNA polymerase subunit omega, at 5,035,982 to 5,036,245 _04423 PFR28_04424: PFR28_04424 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 5,036,303 to 5,038,408 _04424 Position (kb) 5035 5036Strain fitness (log2 ratio) -3 -2 -1 0 1at 5034.224 kb on - strandat 5034.224 kb on - strandat 5034.224 kb on - strandat 5034.255 kb on + strandat 5035.112 kb on + strandat 5035.846 kb on + strandat 5035.846 kb on + strandat 5035.846 kb on + strandat 5035.846 kb on + strandat 5035.846 kb on + strandat 5035.846 kb on + strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.847 kb on - strandat 5035.978 kb on + strandat 5035.978 kb on + strandat 5035.978 kb on + strandat 5035.978 kb on + strandat 5035.978 kb on + strandat 5036.010 kb on + strand, within PFR28_04423at 5036.010 kb on + strand, within PFR28_04423at 5036.162 kb on + strand, within PFR28_04423at 5036.162 kb on + strand, within PFR28_04423at 5036.163 kb on - strand, within PFR28_04423at 5036.243 kb on + strandat 5036.243 kb on + strandat 5036.243 kb on + strandat 5036.249 kb on + strandat 5036.249 kb on + strandat 5036.249 kb on + strandat 5036.313 kb on + strandat 5036.314 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Adenosine (C) 10 mM
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5,034,224 - +0.5
5,034,224 - +0.4
5,034,224 - +0.8
5,034,255 + +1.6
5,035,112 + +1.4
5,035,846 + +1.5
5,035,846 + -0.3
5,035,846 + -0.3
5,035,846 + +0.3
5,035,846 + -0.5
5,035,846 + +0.2
5,035,847 - +0.4
5,035,847 - -0.2
5,035,847 - +0.6
5,035,847 - +0.2
5,035,847 - +0.9
5,035,847 - -0.4
5,035,847 - +0.2
5,035,847 - -0.4
5,035,847 - +1.0
5,035,847 - +0.2
5,035,847 - +0.2
5,035,847 - -0.3
5,035,847 - -0.2
5,035,978 + -1.7
5,035,978 + +1.4
5,035,978 + -3.4
5,035,978 + -0.8
5,035,978 + -3.1
5,036,010 + PFR28_04423 0.11 +0.5
5,036,010 + PFR28_04423 0.11 -0.3
5,036,162 + PFR28_04423 0.68 +0.3
5,036,162 + PFR28_04423 0.68 +0.0
5,036,163 - PFR28_04423 0.69 +0.0
5,036,243 + -0.5
5,036,243 + -1.3
5,036,243 + +0.3
5,036,249 + +0.9
5,036,249 + +0.0
5,036,249 + -1.2
5,036,313 + -0.1
5,036,314 - -1.4

Or see this region's nucleotide sequence