Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_1740 and Echvi_1741 are separated by 18 nucleotides Echvi_1741 and Echvi_1742 overlap by 4 nucleotides Echvi_1742 and Echvi_1743 overlap by 4 nucleotides
Echvi_1740: Echvi_1740 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 2,050,246 to 2,051,475
_1740
Echvi_1741: Echvi_1741 - DNA polymerase III, gamma/tau subunits, at 2,051,494 to 2,052,636
_1741
Echvi_1742: Echvi_1742 - Uncharacterized protein conserved in archaea, at 2,052,633 to 2,053,076
_1742
Echvi_1743: Echvi_1743 - alpha-L-glutamate ligases, RimK family, at 2,053,073 to 2,053,981
_1743
Position (kb)
2051
2052
2053 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2051.473 kb on + strand at 2052.611 kb on + strand at 2052.989 kb on - strand, within Echvi_1742 at 2053.022 kb on - strand, within Echvi_1742 at 2053.031 kb on - strand, within Echvi_1742 at 2053.031 kb on - strand, within Echvi_1742 at 2053.067 kb on + strand at 2053.067 kb on + strand at 2053.086 kb on + strand at 2053.109 kb on + strand at 2053.220 kb on + strand, within Echvi_1743 at 2053.286 kb on + strand, within Echvi_1743 at 2053.286 kb on + strand, within Echvi_1743 at 2053.500 kb on + strand, within Echvi_1743 at 2053.597 kb on + strand, within Echvi_1743
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 2,051,473 + +3.7 2,052,611 + -1.1 2,052,989 - Echvi_1742 0.80 -1.3 2,053,022 - Echvi_1742 0.88 +1.1 2,053,031 - Echvi_1742 0.90 +0.6 2,053,031 - Echvi_1742 0.90 +0.2 2,053,067 + +3.0 2,053,067 + +1.5 2,053,086 + +1.6 2,053,109 + +1.8 2,053,220 + Echvi_1743 0.16 +1.1 2,053,286 + Echvi_1743 0.23 +2.9 2,053,286 + Echvi_1743 0.23 +3.0 2,053,500 + Echvi_1743 0.47 +1.8 2,053,597 + Echvi_1743 0.58 +2.0
Or see this region's nucleotide sequence