Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0722 and Echvi_0723 are separated by 59 nucleotides Echvi_0723 and Echvi_0724 are separated by 30 nucleotides Echvi_0724 and Echvi_0725 overlap by 4 nucleotides
Echvi_0722: Echvi_0722 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 812,825 to 814,201
_0722
Echvi_0723: Echvi_0723 - hypothetical protein, at 814,261 to 814,734
_0723
Echvi_0724: Echvi_0724 - hypothetical protein, at 814,765 to 815,304
_0724
Echvi_0725: Echvi_0725 - hypothetical protein, at 815,301 to 815,741
_0725
Position (kb) 814
815 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.302 kb on + strand, within Echvi_0722 at 813.303 kb on - strand, within Echvi_0722 at 813.307 kb on + strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.308 kb on - strand, within Echvi_0722 at 813.372 kb on - strand, within Echvi_0722 at 813.548 kb on + strand, within Echvi_0722 at 813.562 kb on + strand, within Echvi_0722 at 813.600 kb on + strand, within Echvi_0722 at 813.601 kb on - strand, within Echvi_0722 at 813.654 kb on + strand, within Echvi_0722 at 813.740 kb on + strand, within Echvi_0722 at 813.740 kb on + strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.741 kb on - strand, within Echvi_0722 at 813.788 kb on + strand, within Echvi_0722 at 813.871 kb on - strand, within Echvi_0722 at 813.874 kb on + strand, within Echvi_0722 at 813.875 kb on - strand, within Echvi_0722 at 813.875 kb on - strand, within Echvi_0722 at 813.875 kb on - strand, within Echvi_0722 at 813.941 kb on - strand, within Echvi_0722 at 813.950 kb on - strand, within Echvi_0722 at 813.981 kb on + strand, within Echvi_0722 at 813.982 kb on - strand, within Echvi_0722 at 813.982 kb on - strand, within Echvi_0722 at 813.982 kb on - strand, within Echvi_0722 at 814.108 kb on - strand at 814.121 kb on + strand at 814.122 kb on - strand at 814.122 kb on - strand at 814.122 kb on - strand at 814.122 kb on - strand at 814.126 kb on + strand at 814.126 kb on + strand at 814.126 kb on + strand at 814.320 kb on + strand, within Echvi_0723 at 814.321 kb on - strand, within Echvi_0723 at 814.388 kb on + strand, within Echvi_0723 at 814.389 kb on - strand, within Echvi_0723 at 814.436 kb on + strand, within Echvi_0723 at 814.437 kb on - strand, within Echvi_0723 at 814.509 kb on + strand, within Echvi_0723 at 814.652 kb on + strand, within Echvi_0723 at 814.663 kb on + strand, within Echvi_0723 at 814.672 kb on - strand, within Echvi_0723 at 814.715 kb on - strand at 814.735 kb on - strand at 814.755 kb on - strand at 814.755 kb on - strand at 814.771 kb on - strand at 814.771 kb on - strand at 814.890 kb on + strand, within Echvi_0724 at 814.992 kb on - strand, within Echvi_0724 at 814.992 kb on - strand, within Echvi_0724 at 814.998 kb on + strand, within Echvi_0724 at 815.027 kb on + strand, within Echvi_0724 at 815.183 kb on + strand, within Echvi_0724 at 815.183 kb on + strand, within Echvi_0724 at 815.184 kb on - strand, within Echvi_0724 at 815.184 kb on - strand, within Echvi_0724 at 815.285 kb on - strand at 815.333 kb on + strand at 815.334 kb on - strand at 815.406 kb on - strand, within Echvi_0725 at 815.701 kb on + strand at 815.704 kb on + strand at 815.704 kb on + strand at 815.704 kb on + strand at 815.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 813,302 + Echvi_0722 0.35 -2.2 813,302 + Echvi_0722 0.35 +0.3 813,302 + Echvi_0722 0.35 +0.6 813,302 + Echvi_0722 0.35 +0.7 813,303 - Echvi_0722 0.35 +1.7 813,307 + Echvi_0722 0.35 -0.7 813,308 - Echvi_0722 0.35 -0.5 813,308 - Echvi_0722 0.35 +2.2 813,308 - Echvi_0722 0.35 -2.9 813,372 - Echvi_0722 0.40 -2.1 813,548 + Echvi_0722 0.53 +0.9 813,562 + Echvi_0722 0.54 +1.3 813,600 + Echvi_0722 0.56 -2.3 813,601 - Echvi_0722 0.56 +1.1 813,654 + Echvi_0722 0.60 -0.2 813,740 + Echvi_0722 0.66 +2.5 813,740 + Echvi_0722 0.66 -0.3 813,741 - Echvi_0722 0.67 -1.7 813,741 - Echvi_0722 0.67 +0.1 813,741 - Echvi_0722 0.67 -3.3 813,741 - Echvi_0722 0.67 -0.4 813,741 - Echvi_0722 0.67 -1.4 813,788 + Echvi_0722 0.70 -1.2 813,871 - Echvi_0722 0.76 -0.5 813,874 + Echvi_0722 0.76 +1.1 813,875 - Echvi_0722 0.76 +0.3 813,875 - Echvi_0722 0.76 +2.6 813,875 - Echvi_0722 0.76 -1.0 813,941 - Echvi_0722 0.81 -2.5 813,950 - Echvi_0722 0.82 +0.4 813,981 + Echvi_0722 0.84 -1.2 813,982 - Echvi_0722 0.84 +1.8 813,982 - Echvi_0722 0.84 -1.0 813,982 - Echvi_0722 0.84 -2.8 814,108 - -2.3 814,121 + -1.3 814,122 - -1.2 814,122 - +2.4 814,122 - -1.2 814,122 - -2.2 814,126 + -2.0 814,126 + -1.3 814,126 + +2.2 814,320 + Echvi_0723 0.12 -1.0 814,321 - Echvi_0723 0.13 -1.0 814,388 + Echvi_0723 0.27 +7.2 814,389 - Echvi_0723 0.27 -2.2 814,436 + Echvi_0723 0.37 +0.1 814,437 - Echvi_0723 0.37 -0.7 814,509 + Echvi_0723 0.52 -2.9 814,652 + Echvi_0723 0.82 +0.6 814,663 + Echvi_0723 0.85 +2.3 814,672 - Echvi_0723 0.87 -0.3 814,715 - -1.1 814,735 - +0.2 814,755 - +0.7 814,755 - -2.6 814,771 - +2.6 814,771 - -0.5 814,890 + Echvi_0724 0.23 +1.8 814,992 - Echvi_0724 0.42 +2.2 814,992 - Echvi_0724 0.42 +2.7 814,998 + Echvi_0724 0.43 +0.9 815,027 + Echvi_0724 0.49 +2.7 815,183 + Echvi_0724 0.77 +2.6 815,183 + Echvi_0724 0.77 +4.0 815,184 - Echvi_0724 0.78 +4.1 815,184 - Echvi_0724 0.78 +2.5 815,285 - +3.2 815,333 + +0.5 815,334 - +1.1 815,406 - Echvi_0725 0.24 -0.8 815,701 + -2.2 815,704 + -1.9 815,704 + -1.5 815,704 + -0.7 815,705 - +0.2
Or see this region's nucleotide sequence