Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0321 and Echvi_0322 overlap by 26 nucleotides Echvi_0322 and Echvi_0323 are separated by 70 nucleotides
Echvi_0321: Echvi_0321 - Glycerol-3-phosphate O-acyltransferase, at 334,654 to 336,354
_0321
Echvi_0322: Echvi_0322 - Glycerol-3-phosphate dehydrogenase, at 336,329 to 337,393
_0322
Echvi_0323: Echvi_0323 - Gluconolactonase, at 337,464 to 338,357
_0323
Position (kb) 336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 335.355 kb on + strand, within Echvi_0321 at 335.583 kb on + strand, within Echvi_0321 at 335.583 kb on + strand, within Echvi_0321 at 335.583 kb on + strand, within Echvi_0321 at 335.583 kb on + strand, within Echvi_0321 at 335.583 kb on + strand, within Echvi_0321 at 335.585 kb on + strand, within Echvi_0321 at 335.586 kb on - strand, within Echvi_0321 at 335.586 kb on - strand, within Echvi_0321 at 335.623 kb on + strand, within Echvi_0321 at 335.623 kb on + strand, within Echvi_0321 at 335.623 kb on + strand, within Echvi_0321 at 335.623 kb on + strand, within Echvi_0321 at 335.623 kb on + strand, within Echvi_0321 at 335.631 kb on + strand, within Echvi_0321 at 335.689 kb on + strand, within Echvi_0321 at 335.692 kb on + strand, within Echvi_0321 at 335.692 kb on + strand, within Echvi_0321 at 335.722 kb on + strand, within Echvi_0321 at 335.812 kb on + strand, within Echvi_0321 at 335.835 kb on + strand, within Echvi_0321 at 335.853 kb on + strand, within Echvi_0321 at 335.853 kb on + strand, within Echvi_0321 at 335.853 kb on + strand, within Echvi_0321 at 335.895 kb on - strand, within Echvi_0321 at 335.895 kb on - strand, within Echvi_0321 at 335.895 kb on - strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.900 kb on + strand, within Echvi_0321 at 335.901 kb on - strand, within Echvi_0321 at 335.901 kb on - strand, within Echvi_0321 at 335.901 kb on - strand, within Echvi_0321 at 335.901 kb on - strand, within Echvi_0321 at 335.923 kb on + strand, within Echvi_0321 at 335.923 kb on + strand, within Echvi_0321 at 335.923 kb on + strand, within Echvi_0321 at 335.924 kb on - strand, within Echvi_0321 at 336.233 kb on - strand at 336.301 kb on + strand at 336.301 kb on + strand at 336.302 kb on - strand at 336.302 kb on - strand at 336.307 kb on + strand at 336.580 kb on - strand, within Echvi_0322 at 336.912 kb on - strand, within Echvi_0322 at 337.154 kb on + strand, within Echvi_0322 at 337.327 kb on + strand at 337.346 kb on + strand at 337.346 kb on + strand at 337.349 kb on + strand at 337.350 kb on - strand at 337.626 kb on + strand, within Echvi_0323 at 337.626 kb on + strand, within Echvi_0323 at 337.626 kb on + strand, within Echvi_0323 at 337.656 kb on + strand, within Echvi_0323 at 337.657 kb on - strand, within Echvi_0323 at 337.657 kb on - strand, within Echvi_0323 at 337.692 kb on + strand, within Echvi_0323 at 337.693 kb on - strand, within Echvi_0323 at 337.725 kb on + strand, within Echvi_0323 at 337.889 kb on + strand, within Echvi_0323 at 337.902 kb on + strand, within Echvi_0323 at 337.903 kb on - strand, within Echvi_0323 at 337.956 kb on + strand, within Echvi_0323 at 337.957 kb on - strand, within Echvi_0323 at 338.080 kb on - strand, within Echvi_0323 at 338.126 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.323 kb on - strand at 338.323 kb on - strand at 338.336 kb on - strand at 338.355 kb on + strand at 338.356 kb on - strand at 338.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 335,355 + Echvi_0321 0.41 -1.5 335,583 + Echvi_0321 0.55 -0.8 335,583 + Echvi_0321 0.55 -3.5 335,583 + Echvi_0321 0.55 -2.3 335,583 + Echvi_0321 0.55 -2.0 335,583 + Echvi_0321 0.55 -1.5 335,585 + Echvi_0321 0.55 -0.7 335,586 - Echvi_0321 0.55 -1.2 335,586 - Echvi_0321 0.55 -3.0 335,623 + Echvi_0321 0.57 +0.8 335,623 + Echvi_0321 0.57 -2.3 335,623 + Echvi_0321 0.57 -2.6 335,623 + Echvi_0321 0.57 -1.4 335,623 + Echvi_0321 0.57 -0.6 335,631 + Echvi_0321 0.57 +0.4 335,689 + Echvi_0321 0.61 -1.7 335,692 + Echvi_0321 0.61 -1.7 335,692 + Echvi_0321 0.61 -1.8 335,722 + Echvi_0321 0.63 +0.1 335,812 + Echvi_0321 0.68 -0.0 335,835 + Echvi_0321 0.69 -2.0 335,853 + Echvi_0321 0.70 -2.9 335,853 + Echvi_0321 0.70 -1.7 335,853 + Echvi_0321 0.70 -0.9 335,895 - Echvi_0321 0.73 -2.2 335,895 - Echvi_0321 0.73 -2.1 335,895 - Echvi_0321 0.73 -2.6 335,900 + Echvi_0321 0.73 +0.1 335,900 + Echvi_0321 0.73 -0.4 335,900 + Echvi_0321 0.73 -1.5 335,900 + Echvi_0321 0.73 -1.3 335,900 + Echvi_0321 0.73 -1.1 335,900 + Echvi_0321 0.73 +1.6 335,900 + Echvi_0321 0.73 -1.0 335,901 - Echvi_0321 0.73 -1.4 335,901 - Echvi_0321 0.73 -2.1 335,901 - Echvi_0321 0.73 -0.4 335,901 - Echvi_0321 0.73 +1.8 335,923 + Echvi_0321 0.75 -0.0 335,923 + Echvi_0321 0.75 -1.3 335,923 + Echvi_0321 0.75 +1.0 335,924 - Echvi_0321 0.75 +0.8 336,233 - -1.0 336,301 + -0.4 336,301 + -2.2 336,302 - -2.3 336,302 - -1.4 336,307 + -2.7 336,580 - Echvi_0322 0.24 +2.4 336,912 - Echvi_0322 0.55 +0.4 337,154 + Echvi_0322 0.77 -0.8 337,327 + -1.0 337,346 + -1.0 337,346 + -2.0 337,349 + -0.2 337,350 - +0.2 337,626 + Echvi_0323 0.18 +1.0 337,626 + Echvi_0323 0.18 +2.4 337,626 + Echvi_0323 0.18 +0.1 337,656 + Echvi_0323 0.21 -1.4 337,657 - Echvi_0323 0.22 -2.7 337,657 - Echvi_0323 0.22 -3.1 337,692 + Echvi_0323 0.26 -1.3 337,693 - Echvi_0323 0.26 -0.5 337,725 + Echvi_0323 0.29 -0.0 337,889 + Echvi_0323 0.48 +1.2 337,902 + Echvi_0323 0.49 +0.2 337,903 - Echvi_0323 0.49 -0.2 337,956 + Echvi_0323 0.55 -1.0 337,957 - Echvi_0323 0.55 +1.1 338,080 - Echvi_0323 0.69 -2.1 338,126 - Echvi_0323 0.74 +1.9 338,179 - Echvi_0323 0.80 -2.5 338,179 - Echvi_0323 0.80 -1.5 338,179 - Echvi_0323 0.80 -0.9 338,179 - Echvi_0323 0.80 +0.4 338,323 - +0.7 338,323 - +1.7 338,336 - +1.3 338,355 + +0.1 338,356 - -1.0 338,378 + +2.1
Or see this region's nucleotide sequence