Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0186 and Echvi_0187 are separated by 18 nucleotides Echvi_0187 and Echvi_0188 are separated by 382 nucleotides Echvi_0188 and Echvi_0189 are separated by 24 nucleotides Echvi_0189 and Echvi_0190 are separated by 100 nucleotides
Echvi_0186: Echvi_0186 - Predicted metal-binding, possibly nucleic acid-binding protein, at 204,142 to 204,708
_0186
Echvi_0187: Echvi_0187 - ribosomal protein L32, at 204,727 to 204,918
_0187
Echvi_0188: Echvi_0188 - 3-oxoacyl-(acyl-carrier-protein) synthase III, at 205,301 to 206,299
_0188
Echvi_0189: Echvi_0189 - translation elongation factor P, at 206,324 to 206,887
_0189
Echvi_0190: Echvi_0190 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 206,988 to 207,473
_0190
Position (kb) 205
206
207 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 204.412 kb on + strand, within Echvi_0186 at 204.512 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.590 kb on + strand, within Echvi_0186 at 204.592 kb on + strand, within Echvi_0186 at 205.010 kb on - strand at 205.023 kb on + strand at 205.032 kb on + strand at 205.146 kb on - strand at 205.229 kb on + strand at 205.239 kb on + strand at 205.239 kb on + strand at 206.307 kb on + strand at 206.307 kb on + strand at 206.916 kb on + strand at 206.920 kb on + strand at 206.926 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 204,412 + Echvi_0186 0.48 -2.4 204,512 + Echvi_0186 0.65 -0.2 204,590 + Echvi_0186 0.79 +1.4 204,590 + Echvi_0186 0.79 -1.8 204,590 + Echvi_0186 0.79 +1.0 204,592 + Echvi_0186 0.79 +2.8 205,010 - -1.5 205,023 + +0.6 205,032 + -3.1 205,146 - -0.6 205,229 + +1.4 205,239 + -2.3 205,239 + +1.1 206,307 + -2.9 206,307 + -0.8 206,916 + -0.9 206,920 + -2.0 206,926 + -0.2
Or see this region's nucleotide sequence