Experiment: m.b. D-Cycloserine 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0089 and Echvi_0090 are separated by 191 nucleotides Echvi_0090 and Echvi_0091 are separated by 324 nucleotides Echvi_0091 and Echvi_0092 are separated by 141 nucleotides
Echvi_0089: Echvi_0089 - methylmalonyl-CoA epimerase, at 89,002 to 89,406
_0089
Echvi_0090: Echvi_0090 - Iron-sulfur cluster assembly accessory protein, at 89,598 to 89,924
_0090
Echvi_0091: Echvi_0091 - glycine cleavage system T protein, at 90,249 to 91,343
_0091
Echvi_0092: Echvi_0092 - Phosphosulfolactate phosphohydrolase and related enzymes, at 91,485 to 92,198
_0092
Position (kb) 90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 89.445 kb on + strand at 89.450 kb on + strand at 89.450 kb on + strand at 89.451 kb on - strand at 89.454 kb on + strand at 89.508 kb on + strand at 89.575 kb on + strand at 89.575 kb on + strand at 89.578 kb on + strand at 89.591 kb on - strand at 89.597 kb on + strand at 89.597 kb on + strand at 89.684 kb on - strand, within Echvi_0090 at 89.761 kb on + strand, within Echvi_0090 at 89.762 kb on - strand, within Echvi_0090 at 89.811 kb on + strand, within Echvi_0090 at 89.944 kb on - strand at 89.944 kb on - strand at 89.946 kb on - strand at 89.949 kb on - strand at 89.951 kb on + strand at 89.952 kb on - strand at 89.975 kb on - strand at 90.084 kb on + strand at 90.085 kb on - strand at 90.085 kb on - strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.111 kb on + strand at 90.112 kb on - strand at 90.112 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.134 kb on - strand at 90.216 kb on - strand at 90.246 kb on + strand at 90.246 kb on + strand at 90.368 kb on + strand, within Echvi_0091 at 90.369 kb on - strand, within Echvi_0091 at 90.423 kb on - strand, within Echvi_0091 at 90.580 kb on - strand, within Echvi_0091 at 90.825 kb on - strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.847 kb on + strand, within Echvi_0091 at 90.848 kb on - strand, within Echvi_0091 at 90.877 kb on + strand, within Echvi_0091 at 91.296 kb on - strand at 91.346 kb on + strand at 91.346 kb on + strand at 91.347 kb on - strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.348 kb on + strand at 91.349 kb on - strand at 91.408 kb on - strand at 91.426 kb on + strand at 91.426 kb on + strand at 91.671 kb on + strand, within Echvi_0092 at 91.671 kb on + strand, within Echvi_0092 at 91.673 kb on + strand, within Echvi_0092 at 91.875 kb on + strand, within Echvi_0092 at 91.876 kb on - strand, within Echvi_0092 at 91.876 kb on - strand, within Echvi_0092 at 91.953 kb on + strand, within Echvi_0092 at 92.189 kb on - strand at 92.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.064 mg/ml remove 89,445 + -3.4 89,450 + -3.2 89,450 + -2.3 89,451 - +1.6 89,454 + -2.6 89,508 + -2.8 89,575 + -0.8 89,575 + -1.2 89,578 + +0.4 89,591 - +4.0 89,597 + +0.7 89,597 + -0.5 89,684 - Echvi_0090 0.26 +3.0 89,761 + Echvi_0090 0.50 +0.6 89,762 - Echvi_0090 0.50 +2.3 89,811 + Echvi_0090 0.65 -0.2 89,944 - -2.7 89,944 - +1.2 89,946 - +0.0 89,949 - -0.6 89,951 + -2.1 89,952 - +1.0 89,975 - +0.7 90,084 + +0.4 90,085 - -2.6 90,085 - -2.5 90,111 + -1.1 90,111 + +0.9 90,111 + +0.9 90,111 + -1.3 90,112 - -1.5 90,112 - +2.1 90,134 - -0.7 90,134 - +2.1 90,134 - +1.7 90,216 - +1.1 90,246 + -0.4 90,246 + +1.3 90,368 + Echvi_0091 0.11 -1.1 90,369 - Echvi_0091 0.11 -0.2 90,423 - Echvi_0091 0.16 +1.6 90,580 - Echvi_0091 0.30 -0.2 90,825 - Echvi_0091 0.53 -0.8 90,847 + Echvi_0091 0.55 -1.5 90,847 + Echvi_0091 0.55 -1.8 90,848 - Echvi_0091 0.55 +0.1 90,877 + Echvi_0091 0.57 +0.1 91,296 - -2.2 91,346 + -1.5 91,346 + -0.2 91,347 - -0.3 91,348 + -0.7 91,348 + +1.2 91,348 + +1.0 91,348 + -0.2 91,349 - +1.4 91,408 - +0.5 91,426 + -1.0 91,426 + +2.2 91,671 + Echvi_0092 0.26 -2.9 91,671 + Echvi_0092 0.26 -1.3 91,673 + Echvi_0092 0.26 -0.4 91,875 + Echvi_0092 0.55 -1.9 91,876 - Echvi_0092 0.55 +0.8 91,876 - Echvi_0092 0.55 -2.5 91,953 + Echvi_0092 0.66 -1.2 92,189 - +0.0 92,223 - -0.8
Or see this region's nucleotide sequence