Experiment: A22 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnM and phnL overlap by 4 nucleotides phnL and phnK are separated by 110 nucleotides phnK and phnJ overlap by 4 nucleotides
b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241
phnM
b4096: phnL - carbon-phosphorus lyase complex subunit (NCBI), at 4,315,238 to 4,315,918
phnL
b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787
phnK
b4098: phnJ - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,784 to 4,317,629
phnJ
Position (kb)
4315
4316 Strain fitness (log2 ratio)
-1
0
1
2 at 4314.357 kb on - strand, within phnM at 4314.363 kb on + strand, within phnM at 4314.396 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.742 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4315.006 kb on - strand, within phnM at 4315.181 kb on - strand at 4315.192 kb on - strand at 4315.192 kb on - strand at 4315.200 kb on - strand at 4315.288 kb on - strand at 4315.408 kb on + strand, within phnL at 4315.507 kb on + strand, within phnL at 4315.516 kb on - strand, within phnL at 4315.516 kb on - strand, within phnL at 4315.585 kb on - strand, within phnL at 4315.585 kb on - strand, within phnL at 4315.720 kb on - strand, within phnL at 4315.740 kb on + strand, within phnL at 4315.740 kb on + strand, within phnL at 4315.751 kb on + strand, within phnL at 4316.095 kb on + strand at 4316.185 kb on + strand, within phnK at 4316.185 kb on + strand, within phnK at 4316.208 kb on - strand, within phnK at 4316.211 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.447 kb on - strand, within phnK at 4316.461 kb on - strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.654 kb on - strand, within phnK at 4316.891 kb on - strand, within phnJ
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.08 mg/ml remove 4,314,357 - phnM b4095 0.22 +0.6 4,314,363 + phnM b4095 0.23 -1.1 4,314,396 + phnM b4095 0.26 +0.7 4,314,445 + phnM b4095 0.30 -0.8 4,314,445 + phnM b4095 0.30 +1.4 4,314,742 + phnM b4095 0.56 +0.1 4,314,819 + phnM b4095 0.63 +0.2 4,314,819 + phnM b4095 0.63 +0.8 4,314,822 - phnM b4095 0.63 +0.8 4,314,822 - phnM b4095 0.63 -0.2 4,314,857 - phnM b4095 0.66 -0.4 4,314,857 - phnM b4095 0.66 +1.4 4,315,006 - phnM b4095 0.79 -1.1 4,315,181 - +1.5 4,315,192 - +0.8 4,315,192 - +1.2 4,315,200 - +0.2 4,315,288 - +0.4 4,315,408 + phnL b4096 0.25 +1.2 4,315,507 + phnL b4096 0.40 +0.6 4,315,516 - phnL b4096 0.41 +1.2 4,315,516 - phnL b4096 0.41 +0.8 4,315,585 - phnL b4096 0.51 +0.8 4,315,585 - phnL b4096 0.51 +1.0 4,315,720 - phnL b4096 0.71 +1.8 4,315,740 + phnL b4096 0.74 +0.8 4,315,740 + phnL b4096 0.74 +1.8 4,315,751 + phnL b4096 0.75 +1.4 4,316,095 + +1.2 4,316,185 + phnK b4097 0.21 -0.8 4,316,185 + phnK b4097 0.21 +0.6 4,316,208 - phnK b4097 0.24 +1.2 4,316,211 + phnK b4097 0.24 +1.7 4,316,242 + phnK b4097 0.28 +0.2 4,316,242 + phnK b4097 0.28 +0.1 4,316,434 + phnK b4097 0.53 -1.4 4,316,434 + phnK b4097 0.53 +1.4 4,316,447 - phnK b4097 0.55 +0.5 4,316,461 - phnK b4097 0.57 +0.7 4,316,564 + phnK b4097 0.70 -1.1 4,316,564 + phnK b4097 0.70 +0.9 4,316,654 - phnK b4097 0.82 +0.2 4,316,891 - phnJ b4098 0.13 -0.1
Or see this region's nucleotide sequence