Experiment: A22 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabB and yeaB are separated by 3 nucleotides yeaB and sdaA are separated by 183 nucleotides
b1812: pabB - para-aminobenzoate synthase component I (NCBI), at 1,892,829 to 1,894,190
pabB
b1813: yeaB - predicted NUDIX hydrolase (NCBI), at 1,894,194 to 1,894,772
yeaB
b1814: sdaA - L-serine deaminase I (NCBI), at 1,894,956 to 1,896,320
sdaA
Position (kb)
1894
1895 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1893.663 kb on + strand, within pabB at 1893.663 kb on - strand, within pabB at 1893.819 kb on + strand, within pabB at 1893.819 kb on + strand, within pabB at 1893.960 kb on - strand, within pabB at 1893.960 kb on - strand, within pabB at 1894.035 kb on + strand, within pabB at 1894.301 kb on - strand, within yeaB at 1894.400 kb on + strand, within yeaB at 1894.406 kb on - strand, within yeaB at 1894.419 kb on + strand, within yeaB at 1894.446 kb on + strand, within yeaB at 1894.456 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.551 kb on - strand, within yeaB at 1894.579 kb on - strand, within yeaB at 1894.629 kb on - strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.686 kb on - strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.735 kb on + strand at 1894.766 kb on + strand at 1894.766 kb on + strand at 1894.804 kb on + strand at 1894.875 kb on - strand at 1894.875 kb on - strand at 1894.890 kb on + strand at 1894.890 kb on + strand at 1894.915 kb on - strand at 1894.961 kb on - strand at 1894.997 kb on + strand at 1895.002 kb on - strand at 1895.028 kb on - strand at 1895.028 kb on - strand at 1895.039 kb on - strand at 1895.039 kb on - strand at 1895.465 kb on + strand, within sdaA at 1895.498 kb on - strand, within sdaA at 1895.540 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.08 mg/ml remove 1,893,663 + pabB b1812 0.61 +0.7 1,893,663 - pabB b1812 0.61 +0.1 1,893,819 + pabB b1812 0.73 +0.3 1,893,819 + pabB b1812 0.73 +0.1 1,893,960 - pabB b1812 0.83 -0.9 1,893,960 - pabB b1812 0.83 -0.9 1,894,035 + pabB b1812 0.89 -0.3 1,894,301 - yeaB b1813 0.18 +0.9 1,894,400 + yeaB b1813 0.36 +1.6 1,894,406 - yeaB b1813 0.37 +0.7 1,894,419 + yeaB b1813 0.39 +0.1 1,894,446 + yeaB b1813 0.44 -0.5 1,894,456 + yeaB b1813 0.45 +0.9 1,894,500 + yeaB b1813 0.53 -0.8 1,894,500 + yeaB b1813 0.53 +0.1 1,894,551 - yeaB b1813 0.62 +1.6 1,894,579 - yeaB b1813 0.66 +1.4 1,894,629 - yeaB b1813 0.75 +1.1 1,894,683 + yeaB b1813 0.84 +1.4 1,894,683 + yeaB b1813 0.84 -0.5 1,894,686 - yeaB b1813 0.85 +0.9 1,894,693 + yeaB b1813 0.86 -0.5 1,894,693 + yeaB b1813 0.86 +1.5 1,894,735 + -0.5 1,894,766 + -0.5 1,894,766 + +2.9 1,894,804 + +0.1 1,894,875 - +1.5 1,894,875 - -2.1 1,894,890 + +0.5 1,894,890 + +0.4 1,894,915 - +0.1 1,894,961 - +0.1 1,894,997 + -0.5 1,895,002 - -0.2 1,895,028 - +0.1 1,895,028 - +0.1 1,895,039 - -0.5 1,895,039 - -2.1 1,895,465 + sdaA b1814 0.37 -0.1 1,895,498 - sdaA b1814 0.40 -1.1 1,895,540 - sdaA b1814 0.43 -2.1 1,895,646 - sdaA b1814 0.51 +0.1 1,895,646 - sdaA b1814 0.51 +0.3
Or see this region's nucleotide sequence