Experiment: A22 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcT and ydcU are separated by 0 nucleotides ydcU and ydcV overlap by 11 nucleotides ydcV and ydcW are separated by 21 nucleotides
b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854
ydcT
b1442: ydcU - predicted spermidine/putrescine transporter subunit (NCBI), at 1,511,855 to 1,512,796
ydcU
b1443: ydcV - predicted spermidine/putrescine transporter subunit (NCBI), at 1,512,786 to 1,513,580
ydcV
b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026
ydcW
Position (kb)
1512
1513
1514 Strain fitness (log2 ratio)
-2
-1
0
1 at 1511.886 kb on + strand at 1511.886 kb on + strand at 1512.028 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.133 kb on + strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.194 kb on + strand, within ydcU at 1512.269 kb on - strand, within ydcU at 1512.470 kb on - strand, within ydcU at 1512.754 kb on - strand at 1512.754 kb on - strand at 1512.889 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1513.150 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1514.101 kb on + strand, within ydcW at 1514.310 kb on - strand, within ydcW at 1514.401 kb on + strand, within ydcW at 1514.401 kb on + strand, within ydcW at 1514.435 kb on + strand, within ydcW at 1514.503 kb on + strand, within ydcW
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.08 mg/ml remove 1,511,886 + -0.1 1,511,886 + -0.2 1,512,028 - ydcU b1442 0.18 +0.7 1,512,111 - ydcU b1442 0.27 -0.5 1,512,111 - ydcU b1442 0.27 +0.0 1,512,133 + ydcU b1442 0.30 -0.3 1,512,192 - ydcU b1442 0.36 +0.9 1,512,192 - ydcU b1442 0.36 -0.7 1,512,194 + ydcU b1442 0.36 +0.6 1,512,269 - ydcU b1442 0.44 +0.8 1,512,470 - ydcU b1442 0.65 -2.7 1,512,754 - +0.3 1,512,754 - +0.3 1,512,889 - ydcV b1443 0.13 +1.3 1,512,964 - ydcV b1443 0.22 +1.3 1,512,964 - ydcV b1443 0.22 -0.1 1,513,150 - ydcV b1443 0.46 +1.0 1,513,161 - ydcV b1443 0.47 +0.8 1,513,161 - ydcV b1443 0.47 +0.7 1,513,229 + ydcV b1443 0.56 +1.3 1,513,229 + ydcV b1443 0.56 +0.7 1,514,101 + ydcW b1444 0.35 +0.1 1,514,310 - ydcW b1444 0.50 +0.1 1,514,401 + ydcW b1444 0.56 +0.3 1,514,401 + ydcW b1444 0.56 -0.3 1,514,435 + ydcW b1444 0.58 +1.3 1,514,503 + ydcW b1444 0.63 +0.3
Or see this region's nucleotide sequence