Strain Fitness in Escherichia coli BW25113 around b1392

Experiment: A22 0.08 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaC and paaD are separated by 14 nucleotidespaaD and paaE are separated by 7 nucleotidespaaE and paaF overlap by 4 nucleotidespaaF and paaG overlap by 1 nucleotides b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934 paaC b1391: paaD - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (RefSeq), at 1,453,949 to 1,454,446 paaD b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524 paaE b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288 paaF b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076 paaG Position (kb) 1454 1455 1456Strain fitness (log2 ratio) -1 0 1 2at 1453.483 kb on - strand, within paaCat 1453.635 kb on - strand, within paaCat 1453.641 kb on + strand, within paaCat 1453.689 kb on + strand, within paaCat 1453.840 kb on - strand, within paaCat 1454.381 kb on + strand, within paaDat 1454.381 kb on + strand, within paaDat 1454.398 kb on - strandat 1454.470 kb on + strandat 1454.532 kb on - strandat 1454.714 kb on + strand, within paaEat 1454.714 kb on + strand, within paaEat 1454.786 kb on - strand, within paaEat 1455.114 kb on + strand, within paaEat 1455.315 kb on + strand, within paaEat 1455.392 kb on - strand, within paaEat 1455.429 kb on - strandat 1455.610 kb on + strand, within paaFat 1456.073 kb on + strand, within paaFat 1456.216 kb on - strandat 1456.216 kb on - strandat 1456.490 kb on + strand, within paaG

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.08 mg/ml
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1,453,483 - paaC b1390 0.39 -0.2
1,453,635 - paaC b1390 0.60 +0.6
1,453,641 + paaC b1390 0.61 +2.1
1,453,689 + paaC b1390 0.67 -0.3
1,453,840 - paaC b1390 0.87 +0.3
1,454,381 + paaD b1391 0.87 +0.7
1,454,381 + paaD b1391 0.87 -0.6
1,454,398 - +0.0
1,454,470 + +1.0
1,454,532 - +1.4
1,454,714 + paaE b1392 0.24 -0.5
1,454,714 + paaE b1392 0.24 -1.3
1,454,786 - paaE b1392 0.31 -1.0
1,455,114 + paaE b1392 0.62 +0.1
1,455,315 + paaE b1392 0.80 -0.3
1,455,392 - paaE b1392 0.88 -0.5
1,455,429 - +0.7
1,455,610 + paaF b1393 0.12 +0.4
1,456,073 + paaF b1393 0.72 +2.0
1,456,216 - -1.2
1,456,216 - +1.4
1,456,490 + paaG b1394 0.26 -1.0

Or see this region's nucleotide sequence