Experiment: A22 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaC and paaD are separated by 14 nucleotides paaD and paaE are separated by 7 nucleotides paaE and paaF overlap by 4 nucleotides paaF and paaG overlap by 1 nucleotides
b1390: paaC - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,453,188 to 1,453,934
paaC
b1391: paaD - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (RefSeq), at 1,453,949 to 1,454,446
paaD
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288
paaF
b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076
paaG
Position (kb)
1454
1455
1456 Strain fitness (log2 ratio)
-1
0
1
2 at 1453.483 kb on - strand, within paaC at 1453.635 kb on - strand, within paaC at 1453.641 kb on + strand, within paaC at 1453.689 kb on + strand, within paaC at 1453.840 kb on - strand, within paaC at 1454.381 kb on + strand, within paaD at 1454.381 kb on + strand, within paaD at 1454.398 kb on - strand at 1454.470 kb on + strand at 1454.532 kb on - strand at 1454.714 kb on + strand, within paaE at 1454.714 kb on + strand, within paaE at 1454.786 kb on - strand, within paaE at 1455.114 kb on + strand, within paaE at 1455.315 kb on + strand, within paaE at 1455.392 kb on - strand, within paaE at 1455.429 kb on - strand at 1455.610 kb on + strand, within paaF at 1456.073 kb on + strand, within paaF at 1456.216 kb on - strand at 1456.216 kb on - strand at 1456.490 kb on + strand, within paaG
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.08 mg/ml remove 1,453,483 - paaC b1390 0.39 -0.2 1,453,635 - paaC b1390 0.60 +0.6 1,453,641 + paaC b1390 0.61 +2.1 1,453,689 + paaC b1390 0.67 -0.3 1,453,840 - paaC b1390 0.87 +0.3 1,454,381 + paaD b1391 0.87 +0.7 1,454,381 + paaD b1391 0.87 -0.6 1,454,398 - +0.0 1,454,470 + +1.0 1,454,532 - +1.4 1,454,714 + paaE b1392 0.24 -0.5 1,454,714 + paaE b1392 0.24 -1.3 1,454,786 - paaE b1392 0.31 -1.0 1,455,114 + paaE b1392 0.62 +0.1 1,455,315 + paaE b1392 0.80 -0.3 1,455,392 - paaE b1392 0.88 -0.5 1,455,429 - +0.7 1,455,610 + paaF b1393 0.12 +0.4 1,456,073 + paaF b1393 0.72 +2.0 1,456,216 - -1.2 1,456,216 - +1.4 1,456,490 + paaG b1394 0.26 -1.0
Or see this region's nucleotide sequence