Experiment: A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjS and ycjT overlap by 4 nucleotides ycjT and ycjU overlap by 4 nucleotides ycjU and ycjV are separated by 13 nucleotides
b1315: ycjS - predicted oxidoreductase, NADH-binding (NCBI), at 1,374,856 to 1,375,911
ycjS
b1316: ycjT - predicted hydrolase (NCBI), at 1,375,908 to 1,378,175
ycjT
b1317: ycjU - predicted beta-phosphoglucomutase (NCBI), at 1,378,172 to 1,378,831
ycjU
b4524: ycjV - no description, at 1,378,845 to 1,379,926
ycjV
Position (kb)
1375
1376
1377
1378
1379 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1375.200 kb on + strand, within ycjS at 1375.420 kb on + strand, within ycjS at 1375.426 kb on - strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.566 kb on + strand, within ycjS at 1375.701 kb on + strand, within ycjS at 1375.709 kb on - strand, within ycjS at 1375.709 kb on - strand, within ycjS at 1375.842 kb on - strand at 1375.872 kb on + strand at 1375.872 kb on + strand at 1375.985 kb on + strand at 1375.985 kb on + strand at 1376.172 kb on + strand, within ycjT at 1376.392 kb on - strand, within ycjT at 1376.562 kb on - strand, within ycjT at 1376.589 kb on - strand, within ycjT at 1376.589 kb on - strand, within ycjT at 1377.174 kb on - strand, within ycjT at 1377.634 kb on + strand, within ycjT at 1377.666 kb on - strand, within ycjT at 1377.676 kb on - strand, within ycjT at 1377.844 kb on + strand, within ycjT at 1377.850 kb on - strand, within ycjT at 1377.854 kb on + strand, within ycjT at 1377.967 kb on + strand at 1378.082 kb on + strand at 1378.082 kb on + strand at 1378.093 kb on + strand at 1378.093 kb on + strand at 1378.273 kb on - strand, within ycjU at 1378.326 kb on - strand, within ycjU at 1378.659 kb on - strand, within ycjU at 1378.707 kb on + strand, within ycjU at 1378.711 kb on - strand, within ycjU at 1378.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.04 mg/ml remove 1,375,200 + ycjS b1315 0.33 +0.1 1,375,420 + ycjS b1315 0.53 +0.7 1,375,426 - ycjS b1315 0.54 +0.6 1,375,566 + ycjS b1315 0.67 -1.3 1,375,566 + ycjS b1315 0.67 -0.2 1,375,566 + ycjS b1315 0.67 -1.4 1,375,701 + ycjS b1315 0.80 +0.2 1,375,709 - ycjS b1315 0.81 +0.0 1,375,709 - ycjS b1315 0.81 +0.2 1,375,842 - +0.4 1,375,872 + +1.2 1,375,872 + -0.6 1,375,985 + -1.3 1,375,985 + -0.7 1,376,172 + ycjT b1316 0.12 +0.7 1,376,392 - ycjT b1316 0.21 -0.4 1,376,562 - ycjT b1316 0.29 +1.0 1,376,589 - ycjT b1316 0.30 +0.2 1,376,589 - ycjT b1316 0.30 +3.0 1,377,174 - ycjT b1316 0.56 -0.1 1,377,634 + ycjT b1316 0.76 +0.7 1,377,666 - ycjT b1316 0.78 +0.5 1,377,676 - ycjT b1316 0.78 -0.2 1,377,844 + ycjT b1316 0.85 +0.4 1,377,850 - ycjT b1316 0.86 -1.1 1,377,854 + ycjT b1316 0.86 +1.3 1,377,967 + +0.5 1,378,082 + -0.1 1,378,082 + -0.7 1,378,093 + +0.2 1,378,093 + +0.9 1,378,273 - ycjU b1317 0.15 -0.4 1,378,326 - ycjU b1317 0.23 +0.1 1,378,659 - ycjU b1317 0.74 +0.2 1,378,707 + ycjU b1317 0.81 -0.9 1,378,711 - ycjU b1317 0.82 -0.6 1,378,887 + -1.2
Or see this region's nucleotide sequence