Experiment: A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiF and caiE are separated by 85 nucleotides caiE and caiD are separated by 5 nucleotides caiD and caiC are separated by 108 nucleotides
b0034: caiF - transcriptional regulator of cai operon (VIMSS), at 34,300 to 34,695
caiF
b0035: caiE - possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS), at 34,781 to 35,371
caiE
b0036: caiD - crotonobetainyl CoA hydratase (RefSeq), at 35,377 to 36,162
caiD
b0037: caiC - predicted crotonobetaine CoA ligase:carnitine CoA ligase (RefSeq), at 36,271 to 37,824
caiC
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 34.386 kb on + strand, within caiF at 34.386 kb on + strand, within caiF at 34.405 kb on + strand, within caiF at 34.405 kb on + strand, within caiF at 34.413 kb on + strand, within caiF at 34.413 kb on + strand, within caiF at 34.413 kb on + strand, within caiF at 34.413 kb on + strand, within caiF at 34.413 kb on + strand, within caiF at 34.454 kb on + strand, within caiF at 34.454 kb on + strand, within caiF at 34.461 kb on + strand, within caiF at 34.474 kb on + strand, within caiF at 34.482 kb on - strand, within caiF at 34.487 kb on + strand, within caiF at 34.487 kb on + strand, within caiF at 34.492 kb on - strand, within caiF at 34.493 kb on - strand, within caiF at 34.495 kb on - strand, within caiF at 34.495 kb on - strand, within caiF at 34.501 kb on - strand, within caiF at 34.501 kb on - strand, within caiF at 34.507 kb on + strand, within caiF at 34.515 kb on + strand, within caiF at 34.517 kb on - strand, within caiF at 34.517 kb on - strand, within caiF at 34.517 kb on - strand, within caiF at 34.522 kb on - strand, within caiF at 34.527 kb on + strand, within caiF at 34.527 kb on + strand, within caiF at 34.527 kb on + strand, within caiF at 34.534 kb on - strand, within caiF at 34.535 kb on - strand, within caiF at 34.535 kb on - strand, within caiF at 34.535 kb on - strand, within caiF at 34.535 kb on - strand, within caiF at 34.541 kb on + strand, within caiF at 34.545 kb on - strand, within caiF at 34.549 kb on - strand, within caiF at 34.562 kb on - strand, within caiF at 34.562 kb on - strand, within caiF at 34.564 kb on + strand, within caiF at 34.598 kb on + strand, within caiF at 34.598 kb on + strand, within caiF at 34.600 kb on + strand, within caiF at 34.606 kb on - strand, within caiF at 34.606 kb on - strand, within caiF at 34.607 kb on + strand, within caiF at 34.607 kb on + strand, within caiF at 34.619 kb on + strand, within caiF at 34.622 kb on + strand, within caiF at 34.627 kb on - strand, within caiF at 34.639 kb on + strand, within caiF at 34.647 kb on - strand, within caiF at 34.647 kb on - strand, within caiF at 34.651 kb on + strand, within caiF at 34.651 kb on + strand, within caiF at 34.656 kb on + strand at 34.659 kb on - strand at 34.659 kb on - strand at 34.662 kb on + strand at 34.662 kb on + strand at 34.672 kb on + strand at 34.672 kb on + strand at 34.672 kb on + strand at 34.680 kb on + strand at 34.681 kb on + strand at 34.686 kb on - strand at 34.686 kb on - strand at 34.715 kb on + strand at 34.732 kb on + strand at 34.732 kb on + strand at 34.737 kb on + strand at 34.739 kb on + strand at 34.741 kb on + strand at 34.763 kb on - strand at 34.764 kb on + strand at 34.811 kb on + strand at 34.900 kb on - strand, within caiE at 34.942 kb on - strand, within caiE at 35.002 kb on - strand, within caiE at 35.028 kb on + strand, within caiE at 35.028 kb on + strand at 35.028 kb on + strand, within caiE at 35.034 kb on - strand, within caiE at 35.089 kb on - strand, within caiE at 35.108 kb on + strand, within caiE at 35.108 kb on + strand, within caiE at 35.108 kb on + strand, within caiE at 35.166 kb on - strand, within caiE at 35.171 kb on - strand, within caiE at 35.201 kb on - strand, within caiE at 35.243 kb on - strand, within caiE at 35.272 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.349 kb on - strand at 35.428 kb on + strand at 35.467 kb on - strand, within caiD at 35.501 kb on + strand, within caiD at 35.618 kb on - strand, within caiD at 35.640 kb on - strand, within caiD at 35.731 kb on + strand, within caiD at 35.743 kb on + strand, within caiD at 35.857 kb on + strand, within caiD at 35.907 kb on - strand, within caiD at 35.924 kb on - strand, within caiD at 35.924 kb on - strand, within caiD at 35.935 kb on - strand, within caiD at 35.937 kb on + strand, within caiD at 35.937 kb on + strand, within caiD at 35.940 kb on + strand, within caiD at 35.941 kb on - strand, within caiD at 35.963 kb on + strand, within caiD at 35.989 kb on + strand, within caiD at 35.989 kb on - strand, within caiD at 35.989 kb on - strand, within caiD at 35.994 kb on + strand, within caiD at 35.994 kb on - strand, within caiD at 36.004 kb on + strand, within caiD at 36.014 kb on - strand, within caiD at 36.046 kb on + strand, within caiD at 36.052 kb on + strand, within caiD at 36.089 kb on - strand at 36.104 kb on - strand at 36.139 kb on + strand at 36.139 kb on + strand at 36.147 kb on - strand at 36.150 kb on + strand at 36.158 kb on - strand at 36.191 kb on + strand at 36.191 kb on + strand at 36.203 kb on - strand at 36.203 kb on - strand at 36.249 kb on + strand at 36.254 kb on + strand at 36.257 kb on - strand at 36.326 kb on - strand at 36.330 kb on + strand at 36.339 kb on - strand at 36.339 kb on - strand at 36.344 kb on - strand at 36.405 kb on - strand at 36.405 kb on - strand at 36.405 kb on - strand at 36.406 kb on - strand at 36.410 kb on + strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.423 kb on - strand at 36.428 kb on - strand, within caiC at 36.431 kb on + strand, within caiC at 36.441 kb on + strand, within caiC at 36.453 kb on - strand, within caiC at 36.456 kb on + strand, within caiC at 36.460 kb on - strand, within caiC at 36.471 kb on + strand, within caiC at 36.471 kb on + strand, within caiC at 36.522 kb on - strand, within caiC at 36.522 kb on - strand, within caiC at 36.530 kb on - strand, within caiC at 36.530 kb on - strand, within caiC at 36.532 kb on - strand, within caiC at 36.532 kb on - strand, within caiC at 36.542 kb on - strand, within caiC at 36.542 kb on - strand, within caiC at 36.555 kb on - strand, within caiC at 36.565 kb on - strand, within caiC at 36.565 kb on - strand, within caiC at 36.576 kb on - strand, within caiC at 36.576 kb on - strand, within caiC at 36.609 kb on - strand, within caiC at 36.612 kb on - strand, within caiC at 36.612 kb on - strand, within caiC at 36.620 kb on + strand, within caiC at 36.628 kb on - strand, within caiC at 36.628 kb on - strand, within caiC at 36.666 kb on - strand, within caiC at 36.673 kb on - strand, within caiC at 36.759 kb on - strand, within caiC at 36.759 kb on - strand, within caiC at 36.888 kb on + strand, within caiC at 36.888 kb on + strand, within caiC at 36.924 kb on + strand, within caiC at 37.130 kb on + strand, within caiC at 37.155 kb on + strand, within caiC
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.04 mg/ml remove 34,386 + caiF b0034 0.22 +0.4 34,386 + caiF b0034 0.22 +0.1 34,405 + caiF b0034 0.27 -0.0 34,405 + caiF b0034 0.27 -1.0 34,413 + caiF b0034 0.29 -2.0 34,413 + caiF b0034 0.29 -0.3 34,413 + caiF b0034 0.29 -0.0 34,413 + caiF b0034 0.29 +1.3 34,413 + caiF b0034 0.29 -0.8 34,454 + caiF b0034 0.39 +1.0 34,454 + caiF b0034 0.39 +0.2 34,461 + caiF b0034 0.41 +1.0 34,474 + caiF b0034 0.44 +1.6 34,482 - caiF b0034 0.46 +0.8 34,487 + caiF b0034 0.47 -0.0 34,487 + caiF b0034 0.47 +1.0 34,492 - caiF b0034 0.48 +1.0 34,493 - caiF b0034 0.49 -2.8 34,495 - caiF b0034 0.49 +1.0 34,495 - caiF b0034 0.49 -0.2 34,501 - caiF b0034 0.51 +0.6 34,501 - caiF b0034 0.51 +2.3 34,507 + caiF b0034 0.52 -1.0 34,515 + caiF b0034 0.54 -1.0 34,517 - caiF b0034 0.55 +1.7 34,517 - caiF b0034 0.55 +0.2 34,517 - caiF b0034 0.55 +0.6 34,522 - caiF b0034 0.56 +0.5 34,527 + caiF b0034 0.57 -0.5 34,527 + caiF b0034 0.57 -0.0 34,527 + caiF b0034 0.57 -0.0 34,534 - caiF b0034 0.59 -0.0 34,535 - caiF b0034 0.59 -0.0 34,535 - caiF b0034 0.59 -0.4 34,535 - caiF b0034 0.59 +0.4 34,535 - caiF b0034 0.59 +0.4 34,541 + caiF b0034 0.61 +0.5 34,545 - caiF b0034 0.62 -0.6 34,549 - caiF b0034 0.63 -0.0 34,562 - caiF b0034 0.66 -0.3 34,562 - caiF b0034 0.66 -0.5 34,564 + caiF b0034 0.67 +1.0 34,598 + caiF b0034 0.75 -0.7 34,598 + caiF b0034 0.75 -0.2 34,600 + caiF b0034 0.76 -1.6 34,606 - caiF b0034 0.77 +2.0 34,606 - caiF b0034 0.77 +0.7 34,607 + caiF b0034 0.78 +1.0 34,607 + caiF b0034 0.78 -0.0 34,619 + caiF b0034 0.81 -0.0 34,622 + caiF b0034 0.81 +1.6 34,627 - caiF b0034 0.83 -0.2 34,639 + caiF b0034 0.86 -0.6 34,647 - caiF b0034 0.88 -0.0 34,647 - caiF b0034 0.88 -0.0 34,651 + caiF b0034 0.89 -0.9 34,651 + caiF b0034 0.89 -0.0 34,656 + -1.0 34,659 - +0.3 34,659 - -1.9 34,662 + -0.0 34,662 + +1.0 34,672 + +0.1 34,672 + -1.8 34,672 + +1.0 34,680 + -0.7 34,681 + +0.4 34,686 - +0.6 34,686 - -0.0 34,715 + +1.3 34,732 + +0.9 34,732 + -0.0 34,737 + -0.6 34,739 + -1.6 34,741 + +0.5 34,763 - -2.3 34,764 + -0.2 34,811 + -1.0 34,900 - caiE b0035 0.20 +1.2 34,942 - caiE b0035 0.27 -0.0 35,002 - caiE b0035 0.37 -1.0 35,028 + caiE b0035 0.42 +1.1 35,028 + +2.6 35,028 + caiE b0035 0.42 -0.2 35,034 - caiE b0035 0.43 -1.0 35,089 - caiE b0035 0.52 +0.7 35,108 + caiE b0035 0.55 -0.0 35,108 + caiE b0035 0.55 +0.5 35,108 + caiE b0035 0.55 +0.2 35,166 - caiE b0035 0.65 +0.6 35,171 - caiE b0035 0.66 -0.0 35,201 - caiE b0035 0.71 +1.0 35,243 - caiE b0035 0.78 +0.2 35,272 - caiE b0035 0.83 -1.3 35,312 - caiE b0035 0.90 +0.3 35,312 - caiE b0035 0.90 -0.3 35,312 - caiE b0035 0.90 -0.0 35,349 - -0.3 35,428 + +1.0 35,467 - caiD b0036 0.11 -0.8 35,501 + caiD b0036 0.16 -0.6 35,618 - caiD b0036 0.31 -1.2 35,640 - caiD b0036 0.33 -0.0 35,731 + caiD b0036 0.45 -0.0 35,743 + caiD b0036 0.47 +1.7 35,857 + caiD b0036 0.61 +0.6 35,907 - caiD b0036 0.67 -1.0 35,924 - caiD b0036 0.70 -2.0 35,924 - caiD b0036 0.70 +0.6 35,935 - caiD b0036 0.71 -1.0 35,937 + caiD b0036 0.71 -0.0 35,937 + caiD b0036 0.71 -0.5 35,940 + caiD b0036 0.72 -0.7 35,941 - caiD b0036 0.72 +0.2 35,963 + caiD b0036 0.75 -1.0 35,989 + caiD b0036 0.78 -0.6 35,989 - caiD b0036 0.78 +0.5 35,989 - caiD b0036 0.78 -0.7 35,994 + caiD b0036 0.78 -1.6 35,994 - caiD b0036 0.78 +0.3 36,004 + caiD b0036 0.80 -0.0 36,014 - caiD b0036 0.81 -0.2 36,046 + caiD b0036 0.85 -0.7 36,052 + caiD b0036 0.86 -1.0 36,089 - +0.2 36,104 - -1.0 36,139 + -0.0 36,139 + +0.2 36,147 - -0.3 36,150 + +1.6 36,158 - +0.2 36,191 + -0.3 36,191 + +0.6 36,203 - -0.0 36,203 - -0.3 36,249 + +1.0 36,254 + -0.0 36,257 - +1.0 36,326 - -1.0 36,330 + +0.6 36,339 - -1.2 36,339 - -1.0 36,344 - +0.4 36,405 - -0.4 36,405 - -0.5 36,405 - +0.2 36,406 - +1.3 36,410 + +0.6 36,418 - +0.4 36,418 - -0.3 36,423 - +0.6 36,428 - caiC b0037 0.10 +0.1 36,431 + caiC b0037 0.10 +1.7 36,441 + caiC b0037 0.11 +0.2 36,453 - caiC b0037 0.12 +1.2 36,456 + caiC b0037 0.12 -0.0 36,460 - caiC b0037 0.12 +0.1 36,471 + caiC b0037 0.13 +0.6 36,471 + caiC b0037 0.13 -0.0 36,522 - caiC b0037 0.16 -1.0 36,522 - caiC b0037 0.16 -1.8 36,530 - caiC b0037 0.17 -1.0 36,530 - caiC b0037 0.17 -0.7 36,532 - caiC b0037 0.17 +0.1 36,532 - caiC b0037 0.17 -1.2 36,542 - caiC b0037 0.17 +1.0 36,542 - caiC b0037 0.17 -0.0 36,555 - caiC b0037 0.18 -0.0 36,565 - caiC b0037 0.19 +1.7 36,565 - caiC b0037 0.19 -0.3 36,576 - caiC b0037 0.20 +0.4 36,576 - caiC b0037 0.20 -0.8 36,609 - caiC b0037 0.22 +1.6 36,612 - caiC b0037 0.22 -0.9 36,612 - caiC b0037 0.22 +0.1 36,620 + caiC b0037 0.22 +0.3 36,628 - caiC b0037 0.23 -1.1 36,628 - caiC b0037 0.23 -0.6 36,666 - caiC b0037 0.25 -0.5 36,673 - caiC b0037 0.26 +0.1 36,759 - caiC b0037 0.31 -0.8 36,759 - caiC b0037 0.31 -0.2 36,888 + caiC b0037 0.40 -1.0 36,888 + caiC b0037 0.40 +1.0 36,924 + caiC b0037 0.42 +1.0 37,130 + caiC b0037 0.55 -1.2 37,155 + caiC b0037 0.57 -1.0
Or see this region's nucleotide sequence