Experiment: m.b. Lomefloxacin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_1740 and Echvi_1741 are separated by 18 nucleotides Echvi_1741 and Echvi_1742 overlap by 4 nucleotides Echvi_1742 and Echvi_1743 overlap by 4 nucleotides
Echvi_1740: Echvi_1740 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 2,050,246 to 2,051,475
_1740
Echvi_1741: Echvi_1741 - DNA polymerase III, gamma/tau subunits, at 2,051,494 to 2,052,636
_1741
Echvi_1742: Echvi_1742 - Uncharacterized protein conserved in archaea, at 2,052,633 to 2,053,076
_1742
Echvi_1743: Echvi_1743 - alpha-L-glutamate ligases, RimK family, at 2,053,073 to 2,053,981
_1743
Position (kb)
2051
2052
2053 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2051.473 kb on + strand at 2052.611 kb on + strand at 2052.989 kb on - strand, within Echvi_1742 at 2053.022 kb on - strand, within Echvi_1742 at 2053.031 kb on - strand, within Echvi_1742 at 2053.031 kb on - strand, within Echvi_1742 at 2053.067 kb on + strand at 2053.067 kb on + strand at 2053.086 kb on + strand at 2053.109 kb on + strand at 2053.220 kb on + strand, within Echvi_1743 at 2053.286 kb on + strand, within Echvi_1743 at 2053.286 kb on + strand, within Echvi_1743 at 2053.500 kb on + strand, within Echvi_1743 at 2053.597 kb on + strand, within Echvi_1743
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Lomefloxacin 0.0125 mM remove 2,051,473 + +0.8 2,052,611 + +1.1 2,052,989 - Echvi_1742 0.80 -2.3 2,053,022 - Echvi_1742 0.88 -0.2 2,053,031 - Echvi_1742 0.90 -0.4 2,053,031 - Echvi_1742 0.90 -0.7 2,053,067 + +0.8 2,053,067 + +1.8 2,053,086 + +0.3 2,053,109 + +0.6 2,053,220 + Echvi_1743 0.16 +1.4 2,053,286 + Echvi_1743 0.23 +1.0 2,053,286 + Echvi_1743 0.23 +1.1 2,053,500 + Echvi_1743 0.47 +1.1 2,053,597 + Echvi_1743 0.58 +1.3
Or see this region's nucleotide sequence