Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkA and mscL are separated by 129 nucleotides mscL and yhdL overlap by 4 nucleotides yhdL and zntR are separated by 55 nucleotides zntR and yhdN are separated by 10 nucleotides yhdN and rplQ are separated by 106 nucleotides
b3290: trkA - potassium transporter peripheral membrane component (NCBI), at 3,434,540 to 3,435,916
trkA
b3291: mscL - large-conductance mechanosensitive channel (NCBI), at 3,436,046 to 3,436,456
mscL
b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671
yhdL
b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152
zntR
b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531
yhdN
b3294: rplQ - 50S ribosomal protein L17 (NCBI), at 3,437,638 to 3,438,021
rplQ
Position (kb)
3436
3437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3435.474 kb on - strand, within trkA at 3435.476 kb on - strand, within trkA at 3435.613 kb on + strand, within trkA at 3435.699 kb on + strand, within trkA at 3435.809 kb on + strand at 3435.840 kb on + strand at 3435.840 kb on + strand at 3435.842 kb on + strand at 3435.877 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.889 kb on + strand at 3435.903 kb on + strand at 3436.020 kb on - strand at 3436.020 kb on - strand at 3436.020 kb on - strand at 3436.026 kb on + strand at 3436.027 kb on + strand at 3436.027 kb on + strand at 3436.037 kb on - strand at 3436.037 kb on - strand at 3436.045 kb on - strand at 3436.047 kb on - strand at 3436.061 kb on - strand at 3436.071 kb on + strand at 3436.071 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.081 kb on + strand at 3436.106 kb on - strand, within mscL at 3436.114 kb on + strand, within mscL at 3436.146 kb on + strand, within mscL at 3436.165 kb on + strand, within mscL at 3436.165 kb on + strand, within mscL at 3436.175 kb on + strand, within mscL at 3436.211 kb on - strand, within mscL at 3436.211 kb on - strand, within mscL at 3436.218 kb on + strand, within mscL at 3436.220 kb on + strand, within mscL at 3436.243 kb on - strand, within mscL at 3436.245 kb on - strand, within mscL at 3436.245 kb on - strand, within mscL at 3436.251 kb on - strand, within mscL at 3436.256 kb on + strand, within mscL at 3436.256 kb on - strand, within mscL at 3436.259 kb on - strand, within mscL at 3436.261 kb on - strand, within mscL at 3436.261 kb on - strand, within mscL at 3436.264 kb on + strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.264 kb on - strand, within mscL at 3436.329 kb on + strand, within mscL at 3436.377 kb on + strand, within mscL at 3436.377 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.380 kb on + strand, within mscL at 3436.398 kb on - strand, within mscL at 3436.429 kb on + strand at 3436.457 kb on - strand at 3436.458 kb on - strand at 3436.460 kb on - strand at 3436.476 kb on + strand, within yhdL at 3436.482 kb on + strand, within yhdL at 3436.482 kb on + strand, within yhdL at 3436.524 kb on - strand, within yhdL at 3436.524 kb on - strand, within yhdL at 3436.528 kb on - strand, within yhdL at 3436.528 kb on - strand, within yhdL at 3436.551 kb on + strand, within yhdL at 3436.551 kb on + strand, within yhdL at 3436.559 kb on - strand, within yhdL at 3436.617 kb on - strand, within yhdL at 3436.636 kb on - strand, within yhdL at 3436.642 kb on - strand, within yhdL at 3436.644 kb on + strand, within yhdL at 3436.659 kb on + strand at 3436.748 kb on + strand at 3436.757 kb on + strand at 3436.767 kb on + strand at 3436.796 kb on - strand, within zntR at 3436.803 kb on + strand, within zntR at 3436.811 kb on - strand, within zntR at 3436.811 kb on - strand, within zntR at 3436.840 kb on + strand, within zntR at 3436.840 kb on + strand, within zntR at 3436.848 kb on + strand, within zntR at 3436.889 kb on + strand, within zntR at 3436.889 kb on + strand, within zntR at 3436.927 kb on + strand, within zntR at 3436.935 kb on - strand, within zntR at 3436.935 kb on - strand, within zntR at 3436.987 kb on - strand, within zntR at 3436.994 kb on - strand, within zntR at 3436.994 kb on - strand, within zntR at 3437.070 kb on - strand, within zntR at 3437.111 kb on - strand at 3437.111 kb on - strand at 3437.158 kb on - strand at 3437.158 kb on - strand at 3437.166 kb on - strand at 3437.223 kb on + strand, within yhdN at 3437.226 kb on + strand, within yhdN at 3437.234 kb on - strand, within yhdN at 3437.276 kb on + strand, within yhdN at 3437.338 kb on + strand, within yhdN at 3437.425 kb on - strand, within yhdN at 3437.425 kb on - strand, within yhdN at 3437.492 kb on + strand, within yhdN at 3437.500 kb on - strand at 3437.500 kb on - strand at 3437.502 kb on - strand at 3437.504 kb on + strand at 3437.529 kb on + strand at 3437.534 kb on - strand at 3437.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 3,435,474 - trkA b3290 0.68 +1.3 3,435,476 - trkA b3290 0.68 +4.9 3,435,613 + trkA b3290 0.78 +3.5 3,435,699 + trkA b3290 0.84 +4.2 3,435,809 + +3.8 3,435,840 + +2.8 3,435,840 + +3.1 3,435,842 + +2.5 3,435,877 + +1.3 3,435,889 + +1.1 3,435,889 + +2.1 3,435,889 + +0.9 3,435,889 + +3.2 3,435,903 + +2.1 3,436,020 - +0.0 3,436,020 - +0.5 3,436,020 - -0.5 3,436,026 + +0.1 3,436,027 + -0.5 3,436,027 + +0.7 3,436,037 - -2.2 3,436,037 - -1.1 3,436,045 - -0.6 3,436,047 - +1.2 3,436,061 - +1.7 3,436,071 + -0.8 3,436,071 + -1.5 3,436,081 + -0.5 3,436,081 + -0.8 3,436,081 + -0.5 3,436,081 + +1.9 3,436,106 - mscL b3291 0.15 +1.2 3,436,114 + mscL b3291 0.17 +2.1 3,436,146 + mscL b3291 0.24 -0.2 3,436,165 + mscL b3291 0.29 -1.2 3,436,165 + mscL b3291 0.29 +1.1 3,436,175 + mscL b3291 0.31 +0.3 3,436,211 - mscL b3291 0.40 -0.2 3,436,211 - mscL b3291 0.40 +0.1 3,436,218 + mscL b3291 0.42 -2.1 3,436,220 + mscL b3291 0.42 -0.8 3,436,243 - mscL b3291 0.48 -1.1 3,436,245 - mscL b3291 0.48 +1.3 3,436,245 - mscL b3291 0.48 -1.8 3,436,251 - mscL b3291 0.50 +1.3 3,436,256 + mscL b3291 0.51 +0.1 3,436,256 - mscL b3291 0.51 +0.1 3,436,259 - mscL b3291 0.52 -0.8 3,436,261 - mscL b3291 0.52 +1.5 3,436,261 - mscL b3291 0.52 -2.5 3,436,264 + mscL b3291 0.53 +0.5 3,436,264 - mscL b3291 0.53 -0.5 3,436,264 - mscL b3291 0.53 +1.1 3,436,264 - mscL b3291 0.53 -0.1 3,436,329 + mscL b3291 0.69 -0.6 3,436,377 + mscL b3291 0.81 -0.5 3,436,377 + mscL b3291 0.81 +0.5 3,436,380 + mscL b3291 0.81 -1.3 3,436,380 + mscL b3291 0.81 +1.7 3,436,380 + mscL b3291 0.81 -0.1 3,436,380 + mscL b3291 0.81 +1.5 3,436,380 + mscL b3291 0.81 -0.8 3,436,398 - mscL b3291 0.86 -2.3 3,436,429 + -0.6 3,436,457 - -0.5 3,436,458 - -2.3 3,436,460 - +1.5 3,436,476 + yhdL b4550 0.11 -1.3 3,436,482 + yhdL b4550 0.13 -0.3 3,436,482 + yhdL b4550 0.13 -0.1 3,436,524 - yhdL b4550 0.32 +0.7 3,436,524 - yhdL b4550 0.32 -0.5 3,436,528 - yhdL b4550 0.34 +0.5 3,436,528 - yhdL b4550 0.34 -0.3 3,436,551 + yhdL b4550 0.45 -0.2 3,436,551 + yhdL b4550 0.45 +0.2 3,436,559 - yhdL b4550 0.48 +0.1 3,436,617 - yhdL b4550 0.75 +0.9 3,436,636 - yhdL b4550 0.84 +0.6 3,436,642 - yhdL b4550 0.86 -0.5 3,436,644 + yhdL b4550 0.87 -0.8 3,436,659 + +0.1 3,436,748 + +1.5 3,436,757 + +1.5 3,436,767 + -0.4 3,436,796 - zntR b3292 0.16 -1.5 3,436,803 + zntR b3292 0.18 -0.1 3,436,811 - zntR b3292 0.20 +0.5 3,436,811 - zntR b3292 0.20 +2.1 3,436,840 + zntR b3292 0.27 -1.3 3,436,840 + zntR b3292 0.27 -0.8 3,436,848 + zntR b3292 0.28 +0.8 3,436,889 + zntR b3292 0.38 +1.5 3,436,889 + zntR b3292 0.38 +0.5 3,436,927 + zntR b3292 0.47 -1.8 3,436,935 - zntR b3292 0.49 -0.2 3,436,935 - zntR b3292 0.49 -2.5 3,436,987 - zntR b3292 0.61 +1.5 3,436,994 - zntR b3292 0.63 -0.1 3,436,994 - zntR b3292 0.63 -0.7 3,437,070 - zntR b3292 0.81 +1.2 3,437,111 - -0.9 3,437,111 - +0.3 3,437,158 - +0.5 3,437,158 - -1.0 3,437,166 - -1.1 3,437,223 + yhdN b3293 0.16 -0.5 3,437,226 + yhdN b3293 0.17 -2.8 3,437,234 - yhdN b3293 0.19 -1.1 3,437,276 + yhdN b3293 0.31 -2.8 3,437,338 + yhdN b3293 0.47 +0.7 3,437,425 - yhdN b3293 0.71 -1.1 3,437,425 - yhdN b3293 0.71 -2.0 3,437,492 + yhdN b3293 0.89 +0.1 3,437,500 - +0.2 3,437,500 - -1.1 3,437,502 - +0.8 3,437,504 + -0.1 3,437,529 + -0.8 3,437,534 - -0.6 3,437,669 - -1.3
Or see this region's nucleotide sequence