Strain Fitness in Escherichia coli BW25113 around b4208

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfklB and cycA are separated by 308 nucleotidescycA and ytfE are separated by 44 nucleotidesytfE and ytfF are separated by 107 nucleotides b4207: fklB - FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS), at 4,426,958 to 4,427,578 fklB b4208: cycA - D-alanine/D-serine/glycine transporter (NCBI), at 4,427,887 to 4,429,299 cycA b4209: ytfE - predicted regulator of cell morphogenesis and cell wall metabolism (NCBI), at 4,429,344 to 4,430,006 ytfE b4210: ytfF - predicted inner membrane protein (RefSeq), at 4,430,114 to 4,431,079 ytfF Position (kb) 4427 4428 4429 4430Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 4426.911 kb on + strandat 4426.911 kb on + strandat 4426.912 kb on + strandat 4426.922 kb on - strandat 4426.922 kb on - strandat 4426.932 kb on + strandat 4426.932 kb on + strandat 4426.933 kb on + strandat 4426.965 kb on - strandat 4427.007 kb on - strandat 4427.007 kb on - strandat 4427.009 kb on + strandat 4427.022 kb on + strand, within fklBat 4427.114 kb on + strand, within fklBat 4427.132 kb on - strand, within fklBat 4427.161 kb on + strand, within fklBat 4427.215 kb on - strand, within fklBat 4427.243 kb on + strand, within fklBat 4427.251 kb on - strand, within fklBat 4427.251 kb on - strand, within fklBat 4427.251 kb on - strand, within fklBat 4427.298 kb on - strandat 4427.298 kb on - strand, within fklBat 4427.345 kb on + strand, within fklBat 4427.371 kb on - strand, within fklBat 4427.371 kb on - strand, within fklBat 4427.371 kb on - strand, within fklBat 4427.381 kb on - strand, within fklBat 4427.394 kb on - strand, within fklBat 4427.394 kb on - strand, within fklBat 4427.415 kb on + strand, within fklBat 4427.415 kb on + strand, within fklBat 4427.541 kb on - strandat 4427.541 kb on - strandat 4427.563 kb on + strandat 4427.563 kb on + strandat 4427.580 kb on - strandat 4427.624 kb on - strandat 4427.710 kb on + strandat 4427.783 kb on + strandat 4427.783 kb on + strandat 4427.788 kb on + strandat 4427.806 kb on - strandat 4427.830 kb on - strandat 4427.830 kb on - strandat 4427.840 kb on + strandat 4427.840 kb on + strandat 4427.840 kb on + strandat 4427.850 kb on + strandat 4427.852 kb on - strandat 4427.852 kb on - strandat 4427.906 kb on - strandat 4427.906 kb on - strandat 4427.906 kb on - strandat 4427.961 kb on + strandat 4427.961 kb on + strandat 4427.990 kb on + strandat 4428.012 kb on - strandat 4428.038 kb on + strand, within cycAat 4428.110 kb on + strand, within cycAat 4428.171 kb on - strand, within cycAat 4428.256 kb on + strand, within cycAat 4428.256 kb on + strand, within cycAat 4428.257 kb on + strand, within cycAat 4428.265 kb on - strand, within cycAat 4428.270 kb on - strand, within cycAat 4428.275 kb on - strand, within cycAat 4428.283 kb on - strand, within cycAat 4428.293 kb on + strand, within cycAat 4428.293 kb on + strand, within cycAat 4428.314 kb on - strand, within cycAat 4428.337 kb on - strand, within cycAat 4428.375 kb on - strand, within cycAat 4428.398 kb on + strand, within cycAat 4428.422 kb on + strand, within cycAat 4428.427 kb on + strand, within cycAat 4428.454 kb on - strand, within cycAat 4428.589 kb on - strand, within cycAat 4428.690 kb on - strand, within cycAat 4428.692 kb on - strand, within cycAat 4428.742 kb on - strand, within cycAat 4428.752 kb on + strand, within cycAat 4428.752 kb on + strand, within cycAat 4428.765 kb on - strand, within cycAat 4428.765 kb on - strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.793 kb on + strand, within cycAat 4428.850 kb on - strand, within cycAat 4428.880 kb on - strand, within cycAat 4428.980 kb on + strand, within cycAat 4428.980 kb on + strand, within cycAat 4428.985 kb on + strand, within cycAat 4429.009 kb on + strand, within cycAat 4429.087 kb on - strand, within cycAat 4429.088 kb on + strand, within cycAat 4429.111 kb on + strand, within cycAat 4429.156 kb on - strand, within cycAat 4429.159 kb on - strandat 4429.176 kb on - strandat 4429.185 kb on - strandat 4429.195 kb on + strandat 4429.221 kb on - strandat 4429.221 kb on - strandat 4429.254 kb on - strandat 4429.280 kb on + strandat 4429.315 kb on + strandat 4429.361 kb on + strandat 4429.373 kb on + strandat 4429.453 kb on + strand, within ytfEat 4429.453 kb on + strand, within ytfEat 4429.463 kb on - strand, within ytfEat 4429.463 kb on - strand, within ytfEat 4429.466 kb on - strand, within ytfEat 4429.501 kb on - strand, within ytfEat 4429.559 kb on + strand, within ytfEat 4429.559 kb on + strand, within ytfEat 4429.631 kb on - strand, within ytfEat 4429.631 kb on - strand, within ytfEat 4429.798 kb on + strand, within ytfEat 4429.821 kb on + strand, within ytfEat 4429.857 kb on - strand, within ytfEat 4429.907 kb on - strand, within ytfEat 4429.935 kb on - strand, within ytfEat 4429.935 kb on - strand, within ytfEat 4430.003 kb on + strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.189 kb on - strandat 4430.196 kb on + strandat 4430.199 kb on - strandat 4430.209 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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4,426,911 + -1.5
4,426,911 + -1.1
4,426,912 + +0.0
4,426,922 - +4.9
4,426,922 - +4.5
4,426,932 + +0.5
4,426,932 + +0.5
4,426,933 + -1.0
4,426,965 - +4.0
4,427,007 - +2.7
4,427,007 - +2.9
4,427,009 + -0.3
4,427,022 + fklB b4207 0.10 -0.5
4,427,114 + fklB b4207 0.25 -1.1
4,427,132 - fklB b4207 0.28 +5.0
4,427,161 + fklB b4207 0.33 +0.7
4,427,215 - fklB b4207 0.41 +2.8
4,427,243 + fklB b4207 0.46 -1.2
4,427,251 - fklB b4207 0.47 +0.5
4,427,251 - fklB b4207 0.47 +3.0
4,427,251 - fklB b4207 0.47 +1.3
4,427,298 - -1.1
4,427,298 - fklB b4207 0.55 -2.1
4,427,345 + fklB b4207 0.62 -1.1
4,427,371 - fklB b4207 0.67 +3.3
4,427,371 - fklB b4207 0.67 -0.5
4,427,371 - fklB b4207 0.67 +0.8
4,427,381 - fklB b4207 0.68 +1.5
4,427,394 - fklB b4207 0.70 +2.2
4,427,394 - fklB b4207 0.70 +0.2
4,427,415 + fklB b4207 0.74 +0.7
4,427,415 + fklB b4207 0.74 +0.2
4,427,541 - -0.1
4,427,541 - +1.0
4,427,563 + -1.1
4,427,563 + +0.5
4,427,580 - +0.5
4,427,624 - -1.1
4,427,710 + +1.9
4,427,783 + +1.9
4,427,783 + +1.5
4,427,788 + +1.9
4,427,806 - -0.9
4,427,830 - +1.5
4,427,830 - -0.8
4,427,840 + +1.9
4,427,840 + +1.1
4,427,840 + +3.0
4,427,850 + +1.3
4,427,852 - +0.5
4,427,852 - +0.6
4,427,906 - +1.5
4,427,906 - -1.1
4,427,906 - -2.5
4,427,961 + -1.5
4,427,961 + -1.5
4,427,990 + +0.5
4,428,012 - -0.1
4,428,038 + cycA b4208 0.11 -2.3
4,428,110 + cycA b4208 0.16 -1.1
4,428,171 - cycA b4208 0.20 -3.2
4,428,256 + cycA b4208 0.26 -0.5
4,428,256 + cycA b4208 0.26 -1.9
4,428,257 + cycA b4208 0.26 -1.8
4,428,265 - cycA b4208 0.27 -0.2
4,428,270 - cycA b4208 0.27 -1.9
4,428,275 - cycA b4208 0.27 -2.3
4,428,283 - cycA b4208 0.28 +0.5
4,428,293 + cycA b4208 0.29 -1.5
4,428,293 + cycA b4208 0.29 +0.5
4,428,314 - cycA b4208 0.30 +1.5
4,428,337 - cycA b4208 0.32 -2.1
4,428,375 - cycA b4208 0.35 -2.3
4,428,398 + cycA b4208 0.36 -2.1
4,428,422 + cycA b4208 0.38 -0.3
4,428,427 + cycA b4208 0.38 -1.9
4,428,454 - cycA b4208 0.40 -1.5
4,428,589 - cycA b4208 0.50 -1.1
4,428,690 - cycA b4208 0.57 -1.3
4,428,692 - cycA b4208 0.57 +1.8
4,428,742 - cycA b4208 0.61 +0.5
4,428,752 + cycA b4208 0.61 -1.1
4,428,752 + cycA b4208 0.61 +0.5
4,428,765 - cycA b4208 0.62 -2.3
4,428,765 - cycA b4208 0.62 +0.0
4,428,793 + cycA b4208 0.64 -1.1
4,428,793 + cycA b4208 0.64 -0.5
4,428,793 + cycA b4208 0.64 -1.5
4,428,793 + cycA b4208 0.64 -1.1
4,428,793 + cycA b4208 0.64 +0.5
4,428,793 + cycA b4208 0.64 -1.9
4,428,850 - cycA b4208 0.68 -2.1
4,428,880 - cycA b4208 0.70 -0.8
4,428,980 + cycA b4208 0.77 -1.5
4,428,980 + cycA b4208 0.77 -0.5
4,428,985 + cycA b4208 0.78 -0.5
4,429,009 + cycA b4208 0.79 +0.5
4,429,087 - cycA b4208 0.85 -0.3
4,429,088 + cycA b4208 0.85 -0.9
4,429,111 + cycA b4208 0.87 -0.5
4,429,156 - cycA b4208 0.90 -0.7
4,429,159 - -0.5
4,429,176 - -1.5
4,429,185 - -1.1
4,429,195 + -1.1
4,429,221 - -0.5
4,429,221 - -2.1
4,429,254 - -1.1
4,429,280 + -0.1
4,429,315 + -1.5
4,429,361 + -0.9
4,429,373 + +0.6
4,429,453 + ytfE b4209 0.16 +2.3
4,429,453 + ytfE b4209 0.16 -1.9
4,429,463 - ytfE b4209 0.18 -1.1
4,429,463 - ytfE b4209 0.18 -2.5
4,429,466 - ytfE b4209 0.18 -0.5
4,429,501 - ytfE b4209 0.24 -1.0
4,429,559 + ytfE b4209 0.32 +0.5
4,429,559 + ytfE b4209 0.32 +0.5
4,429,631 - ytfE b4209 0.43 -0.1
4,429,631 - ytfE b4209 0.43 +2.5
4,429,798 + ytfE b4209 0.68 +0.7
4,429,821 + ytfE b4209 0.72 +0.5
4,429,857 - ytfE b4209 0.77 +0.5
4,429,907 - ytfE b4209 0.85 -0.1
4,429,935 - ytfE b4209 0.89 +0.9
4,429,935 - ytfE b4209 0.89 -1.1
4,430,003 + +0.7
4,430,189 - +1.0
4,430,189 - +5.8
4,430,189 - +0.5
4,430,189 - -1.5
4,430,189 - -1.5
4,430,196 + -0.7
4,430,199 - -1.8
4,430,209 - +0.0

Or see this region's nucleotide sequence