Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xdhA and xdhB are separated by 10 nucleotides xdhB and xdhC overlap by 4 nucleotides xdhC and ygeV are separated by 39 nucleotides
b2866: xdhA - xanthine dehydrogenase, molybdenum binding subunit (NCBI), at 2,998,367 to 3,000,625
xdhA
b2867: xdhB - xanthine dehydrogenase, FAD-binding subunit (NCBI), at 3,000,636 to 3,001,514
xdhB
b2868: xdhC - xanthine dehydrogenase, Fe-S binding subunit (NCBI), at 3,001,511 to 3,001,990
xdhC
b2869: ygeV - predicted DNA-binding transcriptional regulator (NCBI), at 3,002,030 to 3,003,808
ygeV
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2999.700 kb on - strand, within xdhA at 2999.765 kb on + strand, within xdhA at 2999.878 kb on - strand, within xdhA at 2999.898 kb on + strand, within xdhA at 2999.898 kb on + strand, within xdhA at 2999.939 kb on + strand, within xdhA at 2999.950 kb on - strand, within xdhA at 2999.950 kb on - strand, within xdhA at 3000.004 kb on + strand, within xdhA at 3000.004 kb on + strand, within xdhA at 3000.080 kb on - strand, within xdhA at 3000.115 kb on - strand, within xdhA at 3000.115 kb on - strand, within xdhA at 3000.119 kb on + strand, within xdhA at 3000.128 kb on + strand, within xdhA at 3000.136 kb on - strand, within xdhA at 3000.228 kb on - strand, within xdhA at 3000.270 kb on + strand, within xdhA at 3000.384 kb on - strand, within xdhA at 3000.421 kb on - strand at 3000.526 kb on + strand at 3000.526 kb on + strand at 3000.536 kb on + strand at 3000.561 kb on + strand at 3000.604 kb on + strand at 3000.656 kb on - strand at 3000.664 kb on + strand at 3000.669 kb on + strand at 3000.730 kb on + strand, within xdhB at 3000.747 kb on - strand, within xdhB at 3000.862 kb on + strand, within xdhB at 3000.862 kb on + strand, within xdhB at 3000.862 kb on + strand, within xdhB at 3000.896 kb on + strand, within xdhB at 3001.048 kb on + strand, within xdhB at 3001.048 kb on + strand, within xdhB at 3001.229 kb on - strand, within xdhB at 3001.290 kb on - strand, within xdhB at 3001.329 kb on - strand, within xdhB at 3001.329 kb on - strand, within xdhB at 3001.468 kb on - strand at 3001.567 kb on + strand, within xdhC at 3001.567 kb on + strand, within xdhC at 3001.616 kb on + strand, within xdhC at 3001.616 kb on + strand, within xdhC at 3001.665 kb on + strand, within xdhC at 3001.678 kb on - strand, within xdhC at 3001.691 kb on - strand, within xdhC at 3001.745 kb on + strand, within xdhC at 3001.756 kb on + strand, within xdhC at 3001.776 kb on + strand, within xdhC at 3001.859 kb on - strand, within xdhC at 3001.940 kb on + strand, within xdhC at 3001.948 kb on - strand at 3002.000 kb on - strand at 3002.034 kb on + strand at 3002.074 kb on + strand at 3002.223 kb on - strand, within ygeV at 3002.224 kb on - strand, within ygeV at 3002.224 kb on - strand, within ygeV at 3002.249 kb on - strand, within ygeV at 3002.254 kb on - strand, within ygeV at 3002.276 kb on + strand, within ygeV at 3002.276 kb on + strand, within ygeV at 3002.293 kb on + strand, within ygeV at 3002.379 kb on + strand, within ygeV at 3002.407 kb on + strand, within ygeV at 3002.436 kb on - strand, within ygeV at 3002.490 kb on - strand, within ygeV at 3002.490 kb on - strand, within ygeV at 3002.490 kb on - strand, within ygeV at 3002.501 kb on + strand, within ygeV at 3002.505 kb on - strand, within ygeV
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 2,999,700 - xdhA b2866 0.59 +0.3 2,999,765 + xdhA b2866 0.62 +0.9 2,999,878 - xdhA b2866 0.67 +0.0 2,999,898 + xdhA b2866 0.68 -1.3 2,999,898 + xdhA b2866 0.68 -0.7 2,999,939 + xdhA b2866 0.70 +1.5 2,999,950 - xdhA b2866 0.70 +1.6 2,999,950 - xdhA b2866 0.70 +0.3 3,000,004 + xdhA b2866 0.72 +1.1 3,000,004 + xdhA b2866 0.72 -2.3 3,000,080 - xdhA b2866 0.76 -0.0 3,000,115 - xdhA b2866 0.77 -0.7 3,000,115 - xdhA b2866 0.77 -1.7 3,000,119 + xdhA b2866 0.78 +0.3 3,000,128 + xdhA b2866 0.78 +0.3 3,000,136 - xdhA b2866 0.78 -1.5 3,000,228 - xdhA b2866 0.82 +0.3 3,000,270 + xdhA b2866 0.84 +0.3 3,000,384 - xdhA b2866 0.89 -0.2 3,000,421 - +1.0 3,000,526 + +0.9 3,000,526 + -1.7 3,000,536 + +1.0 3,000,561 + +0.5 3,000,604 + +0.3 3,000,656 - -2.2 3,000,664 + +1.3 3,000,669 + -1.0 3,000,730 + xdhB b2867 0.11 -2.5 3,000,747 - xdhB b2867 0.13 +0.9 3,000,862 + xdhB b2867 0.26 -0.6 3,000,862 + xdhB b2867 0.26 -0.9 3,000,862 + xdhB b2867 0.26 -0.5 3,000,896 + xdhB b2867 0.30 +1.3 3,001,048 + xdhB b2867 0.47 -2.5 3,001,048 + xdhB b2867 0.47 +0.9 3,001,229 - xdhB b2867 0.67 -0.5 3,001,290 - xdhB b2867 0.74 +0.3 3,001,329 - xdhB b2867 0.79 -3.6 3,001,329 - xdhB b2867 0.79 -1.0 3,001,468 - -0.5 3,001,567 + xdhC b2868 0.12 -0.3 3,001,567 + xdhC b2868 0.12 -0.3 3,001,616 + xdhC b2868 0.22 +0.3 3,001,616 + xdhC b2868 0.22 -0.7 3,001,665 + xdhC b2868 0.32 +1.4 3,001,678 - xdhC b2868 0.35 -0.1 3,001,691 - xdhC b2868 0.38 -0.9 3,001,745 + xdhC b2868 0.49 +1.9 3,001,756 + xdhC b2868 0.51 -0.2 3,001,776 + xdhC b2868 0.55 +0.6 3,001,859 - xdhC b2868 0.72 +0.2 3,001,940 + xdhC b2868 0.89 -0.3 3,001,948 - +0.3 3,002,000 - +0.3 3,002,034 + -0.2 3,002,074 + -0.2 3,002,223 - ygeV b2869 0.11 -1.3 3,002,224 - ygeV b2869 0.11 -0.1 3,002,224 - ygeV b2869 0.11 -1.1 3,002,249 - ygeV b2869 0.12 -0.7 3,002,254 - ygeV b2869 0.13 -1.3 3,002,276 + ygeV b2869 0.14 -0.1 3,002,276 + ygeV b2869 0.14 +1.4 3,002,293 + ygeV b2869 0.15 -1.5 3,002,379 + ygeV b2869 0.20 -0.6 3,002,407 + ygeV b2869 0.21 +0.1 3,002,436 - ygeV b2869 0.23 +0.3 3,002,490 - ygeV b2869 0.26 -0.4 3,002,490 - ygeV b2869 0.26 -0.6 3,002,490 - ygeV b2869 0.26 -0.0 3,002,501 + ygeV b2869 0.26 +0.3 3,002,505 - ygeV b2869 0.27 +0.3
Or see this region's nucleotide sequence