Strain Fitness in Escherichia coli BW25113 around b2451

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyffS and eutA are separated by 148 nucleotideseutA and eutH overlap by 4 nucleotides b2450: yffS - CPZ-55 prophage; predicted protein (RefSeq), at 2,562,545 to 2,563,354 yffS b2451: eutA - reactivating factor for ethanolamine ammonia lyase (NCBI), at 2,563,503 to 2,564,906 eutA b2452: eutH - predicted inner membrane protein (NCBI), at 2,564,903 to 2,566,129 eutH Position (kb) 2563 2564 2565Strain fitness (log2 ratio) -2 -1 0 1 2at 2562.607 kb on - strandat 2562.611 kb on + strandat 2562.611 kb on + strandat 2562.693 kb on - strand, within yffSat 2562.777 kb on + strand, within yffSat 2562.777 kb on + strand, within yffSat 2563.370 kb on - strandat 2563.429 kb on - strandat 2563.754 kb on - strand, within eutAat 2563.754 kb on - strand, within eutAat 2563.862 kb on - strand, within eutAat 2563.862 kb on - strand, within eutAat 2563.913 kb on - strand, within eutAat 2563.949 kb on - strand, within eutAat 2564.001 kb on + strand, within eutAat 2564.037 kb on - strand, within eutAat 2564.285 kb on + strand, within eutAat 2564.377 kb on - strand, within eutAat 2564.443 kb on - strand, within eutAat 2564.443 kb on - strand, within eutAat 2564.498 kb on - strand, within eutAat 2564.498 kb on - strand, within eutAat 2564.577 kb on - strand, within eutAat 2564.599 kb on + strand, within eutAat 2564.626 kb on + strand, within eutAat 2564.636 kb on - strand, within eutAat 2564.636 kb on - strand, within eutAat 2564.741 kb on + strand, within eutAat 2564.743 kb on + strand, within eutAat 2564.753 kb on - strand, within eutAat 2564.865 kb on + strandat 2565.050 kb on + strand, within eutHat 2565.126 kb on + strand, within eutHat 2565.171 kb on - strand, within eutHat 2565.210 kb on - strand, within eutHat 2565.259 kb on - strand, within eutHat 2565.275 kb on + strand, within eutHat 2565.275 kb on + strand, within eutHat 2565.331 kb on + strand, within eutHat 2565.353 kb on - strand, within eutHat 2565.416 kb on + strand, within eutHat 2565.454 kb on - strand, within eutHat 2565.454 kb on - strand, within eutHat 2565.593 kb on + strand, within eutHat 2565.593 kb on + strand, within eutHat 2565.770 kb on - strand, within eutHat 2565.772 kb on - strand, within eutHat 2565.783 kb on - strand, within eutHat 2565.838 kb on - strand, within eutHat 2565.840 kb on + strand, within eutH

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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2,562,607 - +1.0
2,562,611 + -0.7
2,562,611 + -1.4
2,562,693 - yffS b2450 0.18 -0.2
2,562,777 + yffS b2450 0.29 -2.3
2,562,777 + yffS b2450 0.29 +1.2
2,563,370 - +0.0
2,563,429 - -0.3
2,563,754 - eutA b2451 0.18 +0.0
2,563,754 - eutA b2451 0.18 -2.1
2,563,862 - eutA b2451 0.26 +1.0
2,563,862 - eutA b2451 0.26 +0.6
2,563,913 - eutA b2451 0.29 +0.0
2,563,949 - eutA b2451 0.32 -0.5
2,564,001 + eutA b2451 0.35 -0.1
2,564,037 - eutA b2451 0.38 +1.0
2,564,285 + eutA b2451 0.56 +0.0
2,564,377 - eutA b2451 0.62 -0.2
2,564,443 - eutA b2451 0.67 -2.0
2,564,443 - eutA b2451 0.67 +0.0
2,564,498 - eutA b2451 0.71 -0.4
2,564,498 - eutA b2451 0.71 -1.0
2,564,577 - eutA b2451 0.76 +0.8
2,564,599 + eutA b2451 0.78 -1.2
2,564,626 + eutA b2451 0.80 -0.2
2,564,636 - eutA b2451 0.81 -0.8
2,564,636 - eutA b2451 0.81 -1.6
2,564,741 + eutA b2451 0.88 -1.0
2,564,743 + eutA b2451 0.88 -0.7
2,564,753 - eutA b2451 0.89 +0.4
2,564,865 + -0.3
2,565,050 + eutH b2452 0.12 -1.3
2,565,126 + eutH b2452 0.18 +0.4
2,565,171 - eutH b2452 0.22 +0.8
2,565,210 - eutH b2452 0.25 +0.6
2,565,259 - eutH b2452 0.29 -1.1
2,565,275 + eutH b2452 0.30 +0.0
2,565,275 + eutH b2452 0.30 +0.0
2,565,331 + eutH b2452 0.35 -0.4
2,565,353 - eutH b2452 0.37 +0.5
2,565,416 + eutH b2452 0.42 -0.6
2,565,454 - eutH b2452 0.45 +0.2
2,565,454 - eutH b2452 0.45 -0.2
2,565,593 + eutH b2452 0.56 +2.0
2,565,593 + eutH b2452 0.56 -0.3
2,565,770 - eutH b2452 0.71 -0.8
2,565,772 - eutH b2452 0.71 +1.4
2,565,783 - eutH b2452 0.72 +0.8
2,565,838 - eutH b2452 0.76 +1.4
2,565,840 + eutH b2452 0.76 +0.5

Or see this region's nucleotide sequence