Strain Fitness in Escherichia coli BW25113 around b2398

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfeA and alaX are separated by 208 nucleotidesalaX and alaW are separated by 39 nucleotidesalaW and yfeC are separated by 235 nucleotidesyfeC and yfeD are separated by 1 nucleotidesyfeD and gltX are separated by 51 nucleotides b2395: yfeA - orf, hypothetical protein (VIMSS), at 2,513,665 to 2,515,854 yfeA b2396: alaX - tRNA-Ala (NCBI), at 2,516,063 to 2,516,138 alaX b2397: alaW - tRNA-Ala (NCBI), at 2,516,178 to 2,516,253 alaW b2398: yfeC - orf, hypothetical protein (VIMSS), at 2,516,489 to 2,516,833 yfeC b2399: yfeD - predicted DNA-binding transcriptional regulator (RefSeq), at 2,516,835 to 2,517,227 yfeD b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694 gltX Position (kb) 2516 2517Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2515.561 kb on + strand, within yfeAat 2515.744 kb on + strandat 2515.857 kb on + strandat 2515.859 kb on + strandat 2515.859 kb on + strandat 2515.867 kb on - strandat 2515.977 kb on - strandat 2515.986 kb on - strandat 2516.197 kb on - strand, within alaWat 2516.434 kb on - strandat 2516.439 kb on + strandat 2516.439 kb on + strandat 2516.560 kb on - strand, within yfeCat 2516.560 kb on - strand, within yfeCat 2516.568 kb on - strand, within yfeCat 2516.620 kb on - strand, within yfeCat 2516.787 kb on + strand, within yfeCat 2516.808 kb on + strandat 2516.808 kb on + strandat 2516.873 kb on + strandat 2516.873 kb on + strandat 2516.890 kb on - strand, within yfeDat 2516.890 kb on - strand, within yfeDat 2516.963 kb on - strand, within yfeDat 2516.976 kb on - strand, within yfeDat 2517.017 kb on - strand, within yfeDat 2517.027 kb on + strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.035 kb on - strand, within yfeDat 2517.037 kb on - strand, within yfeDat 2517.044 kb on + strand, within yfeDat 2517.044 kb on + strand, within yfeDat 2517.047 kb on + strand, within yfeDat 2517.047 kb on + strand, within yfeDat 2517.203 kb on + strandat 2517.213 kb on + strandat 2517.213 kb on + strandat 2517.281 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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2,515,561 + yfeA b2395 0.87 -0.0
2,515,744 + -1.0
2,515,857 + +0.3
2,515,859 + -0.0
2,515,859 + -2.8
2,515,867 - -0.0
2,515,977 - -0.6
2,515,986 - +0.3
2,516,197 - alaW b2397 0.25 -1.0
2,516,434 - -2.2
2,516,439 + +0.4
2,516,439 + -0.4
2,516,560 - yfeC b2398 0.21 -1.6
2,516,560 - yfeC b2398 0.21 +1.2
2,516,568 - yfeC b2398 0.23 +0.4
2,516,620 - yfeC b2398 0.38 -1.6
2,516,787 + yfeC b2398 0.86 +2.2
2,516,808 + +1.0
2,516,808 + -0.0
2,516,873 + -0.0
2,516,873 + -1.6
2,516,890 - yfeD b2399 0.14 -0.0
2,516,890 - yfeD b2399 0.14 +0.6
2,516,963 - yfeD b2399 0.33 +0.5
2,516,976 - yfeD b2399 0.36 -0.7
2,517,017 - yfeD b2399 0.46 +0.5
2,517,027 + yfeD b2399 0.49 +0.3
2,517,035 - yfeD b2399 0.51 -0.2
2,517,035 - yfeD b2399 0.51 -0.6
2,517,035 - yfeD b2399 0.51 -0.0
2,517,037 - yfeD b2399 0.51 -1.6
2,517,044 + yfeD b2399 0.53 +0.7
2,517,044 + yfeD b2399 0.53 +0.4
2,517,047 + yfeD b2399 0.54 -0.7
2,517,047 + yfeD b2399 0.54 +0.4
2,517,203 + +1.9
2,517,213 + +1.4
2,517,213 + -0.7
2,517,281 - -2.0

Or see this region's nucleotide sequence