Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcM and yfcA overlap by 1 nucleotides yfcA and mepA overlap by 1 nucleotides mepA and aroC are separated by 3 nucleotides
b2326: yfcM - hypothetical protein (NCBI), at 2,442,225 to 2,442,773
yfcM
b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582
yfcA
b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406
mepA
b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495
aroC
Position (kb)
2443
2444
2445 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2442.583 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.647 kb on - strand, within yfcM at 2442.696 kb on - strand, within yfcM at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.758 kb on + strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.766 kb on - strand at 2442.777 kb on - strand at 2442.807 kb on - strand at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.887 kb on + strand, within yfcA at 2442.898 kb on + strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.906 kb on - strand, within yfcA at 2442.948 kb on - strand, within yfcA at 2442.976 kb on + strand, within yfcA at 2442.985 kb on + strand, within yfcA at 2443.071 kb on - strand, within yfcA at 2443.161 kb on + strand, within yfcA at 2443.293 kb on - strand, within yfcA at 2443.330 kb on + strand, within yfcA at 2443.700 kb on - strand, within mepA at 2443.700 kb on - strand, within mepA at 2443.705 kb on + strand, within mepA at 2443.770 kb on + strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.778 kb on - strand, within mepA at 2443.782 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.797 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.817 kb on - strand, within mepA at 2443.897 kb on + strand, within mepA at 2444.082 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.245 kb on - strand, within mepA at 2444.256 kb on + strand, within mepA at 2444.552 kb on - strand, within aroC at 2444.844 kb on + strand, within aroC at 2444.866 kb on - strand, within aroC at 2444.923 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.180 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.244 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.337 kb on - strand, within aroC
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 2,442,583 - yfcM b2326 0.65 +0.1 2,442,647 - yfcM b2326 0.77 -0.5 2,442,647 - yfcM b2326 0.77 +0.2 2,442,696 - yfcM b2326 0.86 -0.6 2,442,758 + +0.4 2,442,758 + -0.6 2,442,758 + +0.4 2,442,766 - -0.0 2,442,766 - -0.7 2,442,766 - +0.7 2,442,766 - -0.6 2,442,777 - -0.0 2,442,807 - +0.6 2,442,887 + yfcA b2327 0.14 -2.0 2,442,887 + yfcA b2327 0.14 -1.4 2,442,887 + yfcA b2327 0.14 -0.3 2,442,887 + yfcA b2327 0.14 +0.6 2,442,898 + yfcA b2327 0.15 +1.0 2,442,906 - yfcA b2327 0.16 -0.0 2,442,906 - yfcA b2327 0.16 -1.6 2,442,948 - yfcA b2327 0.22 +1.1 2,442,976 + yfcA b2327 0.25 -0.6 2,442,985 + yfcA b2327 0.26 -1.0 2,443,071 - yfcA b2327 0.37 -0.0 2,443,161 + yfcA b2327 0.48 +2.0 2,443,293 - yfcA b2327 0.64 -0.4 2,443,330 + yfcA b2327 0.69 -2.4 2,443,700 - mepA b2328 0.14 -1.4 2,443,700 - mepA b2328 0.14 +1.0 2,443,705 + mepA b2328 0.15 -1.0 2,443,770 + mepA b2328 0.23 -0.8 2,443,778 - mepA b2328 0.24 -0.8 2,443,778 - mepA b2328 0.24 -1.6 2,443,782 - mepA b2328 0.24 -1.4 2,443,797 - mepA b2328 0.26 +1.0 2,443,797 - mepA b2328 0.26 -0.3 2,443,817 - mepA b2328 0.28 -3.2 2,443,817 - mepA b2328 0.28 -1.9 2,443,897 + mepA b2328 0.38 -0.3 2,444,082 - mepA b2328 0.61 +0.3 2,444,245 - mepA b2328 0.80 -2.0 2,444,245 - mepA b2328 0.80 +0.4 2,444,256 + mepA b2328 0.82 +0.4 2,444,552 - aroC b2329 0.13 +1.6 2,444,844 + aroC b2329 0.40 +1.3 2,444,866 - aroC b2329 0.42 +2.4 2,444,923 - aroC b2329 0.47 -0.0 2,444,997 - aroC b2329 0.54 +0.7 2,444,997 - aroC b2329 0.54 +2.0 2,445,075 + aroC b2329 0.61 +1.0 2,445,075 + aroC b2329 0.61 +1.0 2,445,180 + aroC b2329 0.71 +1.2 2,445,213 + aroC b2329 0.74 -0.0 2,445,213 + aroC b2329 0.74 -0.6 2,445,219 - aroC b2329 0.74 +1.4 2,445,219 - aroC b2329 0.74 -0.0 2,445,244 + aroC b2329 0.77 +0.5 2,445,326 + aroC b2329 0.84 +1.0 2,445,326 + aroC b2329 0.84 +0.7 2,445,337 - aroC b2329 0.85 -0.0 2,445,337 - aroC b2329 0.85 +2.0
Or see this region's nucleotide sequence