Strain Fitness in Escherichia coli BW25113 around b2305

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfolX and yfcH are separated by 20 nucleotidesyfcH and yfcI are separated by 47 nucleotidesyfcI and hisP are separated by 196 nucleotideshisP and hisM are separated by 7 nucleotides b2303: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI), at 2,419,347 to 2,419,709 folX b2304: yfcH - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,419,730 to 2,420,623 yfcH b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561 yfcI b2306: hisP - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,421,758 to 2,422,531 hisP b2307: hisM - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,422,539 to 2,423,255 hisM Position (kb) 2420 2421 2422Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2419.683 kb on + strandat 2419.689 kb on + strandat 2419.689 kb on + strandat 2419.697 kb on - strandat 2419.720 kb on - strandat 2419.720 kb on - strandat 2419.725 kb on - strandat 2419.726 kb on + strandat 2419.728 kb on + strandat 2419.736 kb on - strandat 2419.736 kb on - strandat 2419.809 kb on + strandat 2419.816 kb on + strandat 2419.816 kb on + strandat 2419.821 kb on + strand, within yfcHat 2419.821 kb on + strand, within yfcHat 2419.865 kb on + strand, within yfcHat 2419.874 kb on - strand, within yfcHat 2420.059 kb on - strand, within yfcHat 2420.077 kb on - strand, within yfcHat 2420.077 kb on - strand, within yfcHat 2420.089 kb on - strand, within yfcHat 2420.225 kb on - strand, within yfcHat 2420.225 kb on - strand, within yfcHat 2420.228 kb on - strand, within yfcHat 2420.228 kb on - strand, within yfcHat 2420.267 kb on + strand, within yfcHat 2420.267 kb on + strand, within yfcHat 2420.303 kb on + strand, within yfcHat 2420.346 kb on + strand, within yfcHat 2420.373 kb on - strand, within yfcHat 2420.373 kb on - strand, within yfcHat 2420.404 kb on - strand, within yfcHat 2420.404 kb on - strand, within yfcHat 2420.450 kb on - strand, within yfcHat 2420.450 kb on - strand, within yfcHat 2420.487 kb on + strand, within yfcHat 2420.487 kb on + strand, within yfcHat 2420.495 kb on - strand, within yfcHat 2420.521 kb on + strand, within yfcHat 2420.526 kb on + strand, within yfcHat 2420.632 kb on + strandat 2420.654 kb on + strandat 2420.677 kb on - strandat 2420.680 kb on - strandat 2420.758 kb on - strandat 2420.758 kb on - strandat 2420.772 kb on + strand, within yfcIat 2420.775 kb on - strand, within yfcIat 2420.775 kb on - strand, within yfcIat 2420.784 kb on - strand, within yfcIat 2420.801 kb on - strand, within yfcIat 2420.932 kb on + strand, within yfcIat 2420.932 kb on + strand, within yfcIat 2420.953 kb on - strand, within yfcIat 2420.966 kb on - strand, within yfcIat 2420.987 kb on + strand, within yfcIat 2420.990 kb on + strand, within yfcIat 2420.990 kb on + strandat 2420.990 kb on + strand, within yfcIat 2420.998 kb on - strand, within yfcIat 2421.000 kb on - strand, within yfcIat 2421.008 kb on - strand, within yfcIat 2421.052 kb on + strand, within yfcIat 2421.052 kb on - strand, within yfcIat 2421.054 kb on + strand, within yfcIat 2421.054 kb on + strand, within yfcIat 2421.055 kb on - strand, within yfcIat 2421.057 kb on + strand, within yfcIat 2421.060 kb on - strand, within yfcIat 2421.060 kb on - strand, within yfcIat 2421.063 kb on - strand, within yfcIat 2421.063 kb on - strand, within yfcIat 2421.075 kb on + strand, within yfcIat 2421.075 kb on + strand, within yfcIat 2421.089 kb on - strand, within yfcIat 2421.089 kb on - strand, within yfcIat 2421.129 kb on + strand, within yfcIat 2421.134 kb on - strand, within yfcIat 2421.146 kb on - strand, within yfcIat 2421.178 kb on - strand, within yfcIat 2421.179 kb on + strand, within yfcIat 2421.179 kb on + strand, within yfcIat 2421.210 kb on + strand, within yfcIat 2421.225 kb on + strand, within yfcIat 2421.225 kb on + strand, within yfcIat 2421.226 kb on + strand, within yfcIat 2421.227 kb on + strand, within yfcIat 2421.227 kb on + strand, within yfcIat 2421.237 kb on + strand, within yfcIat 2421.335 kb on - strand, within yfcIat 2421.345 kb on - strand, within yfcIat 2421.370 kb on - strand, within yfcIat 2421.370 kb on - strand, within yfcIat 2421.385 kb on - strand, within yfcIat 2421.425 kb on - strand, within yfcIat 2421.425 kb on - strand, within yfcIat 2421.425 kb on - strand, within yfcIat 2421.431 kb on - strand, within yfcIat 2421.437 kb on + strand, within yfcIat 2421.439 kb on + strand, within yfcIat 2421.445 kb on + strand, within yfcIat 2421.457 kb on - strand, within yfcIat 2421.457 kb on - strand, within yfcIat 2421.494 kb on + strandat 2421.494 kb on + strandat 2421.502 kb on - strandat 2421.503 kb on - strandat 2421.504 kb on - strandat 2421.535 kb on - strandat 2421.535 kb on - strandat 2421.694 kb on + strandat 2421.771 kb on + strandat 2421.771 kb on + strandat 2421.782 kb on - strandat 2421.790 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.793 kb on - strandat 2421.806 kb on - strandat 2421.860 kb on - strand, within hisPat 2421.874 kb on - strand, within hisPat 2421.918 kb on - strand, within hisPat 2421.921 kb on + strand, within hisPat 2421.928 kb on - strand, within hisPat 2421.928 kb on - strand, within hisPat 2421.963 kb on + strand, within hisPat 2421.963 kb on + strand, within hisPat 2421.971 kb on - strand, within hisPat 2421.973 kb on + strand, within hisPat 2421.981 kb on - strand, within hisPat 2421.981 kb on - strand, within hisPat 2422.005 kb on - strand, within hisPat 2422.199 kb on + strand, within hisPat 2422.199 kb on + strand, within hisPat 2422.218 kb on - strand, within hisPat 2422.232 kb on + strand, within hisPat 2422.232 kb on + strand, within hisPat 2422.255 kb on - strand, within hisPat 2422.255 kb on - strand, within hisPat 2422.375 kb on + strand, within hisPat 2422.486 kb on - strandat 2422.538 kb on - strandat 2422.538 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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2,419,683 + +0.9
2,419,689 + +1.2
2,419,689 + -0.1
2,419,697 - +0.1
2,419,720 - +0.7
2,419,720 - +0.2
2,419,725 - +0.4
2,419,726 + +0.9
2,419,728 + -0.8
2,419,736 - -1.1
2,419,736 - +0.2
2,419,809 + -0.2
2,419,816 + -0.3
2,419,816 + -1.5
2,419,821 + yfcH b2304 0.10 +0.1
2,419,821 + yfcH b2304 0.10 +0.9
2,419,865 + yfcH b2304 0.15 -0.8
2,419,874 - yfcH b2304 0.16 -0.7
2,420,059 - yfcH b2304 0.37 -1.1
2,420,077 - yfcH b2304 0.39 -2.4
2,420,077 - yfcH b2304 0.39 -2.7
2,420,089 - yfcH b2304 0.40 -1.5
2,420,225 - yfcH b2304 0.55 +2.1
2,420,225 - yfcH b2304 0.55 -0.7
2,420,228 - yfcH b2304 0.56 +0.5
2,420,228 - yfcH b2304 0.56 +1.3
2,420,267 + yfcH b2304 0.60 +0.8
2,420,267 + yfcH b2304 0.60 -0.4
2,420,303 + yfcH b2304 0.64 -0.1
2,420,346 + yfcH b2304 0.69 -0.3
2,420,373 - yfcH b2304 0.72 -0.1
2,420,373 - yfcH b2304 0.72 +1.0
2,420,404 - yfcH b2304 0.75 +1.8
2,420,404 - yfcH b2304 0.75 -0.1
2,420,450 - yfcH b2304 0.81 +0.1
2,420,450 - yfcH b2304 0.81 -0.2
2,420,487 + yfcH b2304 0.85 -1.9
2,420,487 + yfcH b2304 0.85 -0.3
2,420,495 - yfcH b2304 0.86 +0.1
2,420,521 + yfcH b2304 0.88 -0.1
2,420,526 + yfcH b2304 0.89 -2.1
2,420,632 + -0.4
2,420,654 + +0.3
2,420,677 - -0.7
2,420,680 - +1.2
2,420,758 - +1.3
2,420,758 - -0.7
2,420,772 + yfcI b2305 0.11 -1.7
2,420,775 - yfcI b2305 0.12 +0.3
2,420,775 - yfcI b2305 0.12 -0.1
2,420,784 - yfcI b2305 0.13 -1.2
2,420,801 - yfcI b2305 0.15 +0.2
2,420,932 + yfcI b2305 0.29 +1.4
2,420,932 + yfcI b2305 0.29 -2.4
2,420,953 - yfcI b2305 0.32 -0.2
2,420,966 - yfcI b2305 0.33 +0.8
2,420,987 + yfcI b2305 0.35 +0.9
2,420,990 + yfcI b2305 0.36 +0.7
2,420,990 + -0.1
2,420,990 + yfcI b2305 0.36 -1.1
2,420,998 - yfcI b2305 0.37 +0.3
2,421,000 - yfcI b2305 0.37 +0.2
2,421,008 - yfcI b2305 0.38 +0.5
2,421,052 + yfcI b2305 0.43 -0.7
2,421,052 - yfcI b2305 0.43 +0.9
2,421,054 + yfcI b2305 0.43 -0.8
2,421,054 + yfcI b2305 0.43 +0.2
2,421,055 - yfcI b2305 0.43 +1.6
2,421,057 + yfcI b2305 0.43 +0.4
2,421,060 - yfcI b2305 0.44 +0.7
2,421,060 - yfcI b2305 0.44 +0.4
2,421,063 - yfcI b2305 0.44 +1.7
2,421,063 - yfcI b2305 0.44 +1.8
2,421,075 + yfcI b2305 0.45 -0.9
2,421,075 + yfcI b2305 0.45 -0.3
2,421,089 - yfcI b2305 0.47 +2.2
2,421,089 - yfcI b2305 0.47 +0.3
2,421,129 + yfcI b2305 0.51 +0.7
2,421,134 - yfcI b2305 0.52 +0.3
2,421,146 - yfcI b2305 0.53 +2.5
2,421,178 - yfcI b2305 0.57 +1.1
2,421,179 + yfcI b2305 0.57 -1.7
2,421,179 + yfcI b2305 0.57 +0.6
2,421,210 + yfcI b2305 0.60 -0.7
2,421,225 + yfcI b2305 0.62 -0.4
2,421,225 + yfcI b2305 0.62 +0.7
2,421,226 + yfcI b2305 0.62 +0.8
2,421,227 + yfcI b2305 0.62 +0.3
2,421,227 + yfcI b2305 0.62 -2.4
2,421,237 + yfcI b2305 0.64 -0.1
2,421,335 - yfcI b2305 0.75 -0.9
2,421,345 - yfcI b2305 0.76 -0.7
2,421,370 - yfcI b2305 0.78 -1.9
2,421,370 - yfcI b2305 0.78 +0.2
2,421,385 - yfcI b2305 0.80 +0.6
2,421,425 - yfcI b2305 0.85 +0.4
2,421,425 - yfcI b2305 0.85 -0.3
2,421,425 - yfcI b2305 0.85 +0.0
2,421,431 - yfcI b2305 0.85 +0.3
2,421,437 + yfcI b2305 0.86 -0.1
2,421,439 + yfcI b2305 0.86 +1.8
2,421,445 + yfcI b2305 0.87 +0.7
2,421,457 - yfcI b2305 0.88 +2.7
2,421,457 - yfcI b2305 0.88 +1.5
2,421,494 + +0.9
2,421,494 + -1.7
2,421,502 - +0.9
2,421,503 - -0.6
2,421,504 - +1.5
2,421,535 - -1.7
2,421,535 - +2.2
2,421,694 + -0.1
2,421,771 + +0.9
2,421,771 + +1.2
2,421,782 - -3.5
2,421,790 - -0.6
2,421,793 - -0.1
2,421,793 - -0.1
2,421,793 - -1.7
2,421,793 - +0.9
2,421,806 - -1.4
2,421,860 - hisP b2306 0.13 -0.6
2,421,874 - hisP b2306 0.15 -0.6
2,421,918 - hisP b2306 0.21 +0.4
2,421,921 + hisP b2306 0.21 +0.3
2,421,928 - hisP b2306 0.22 +1.4
2,421,928 - hisP b2306 0.22 +0.1
2,421,963 + hisP b2306 0.26 +0.2
2,421,963 + hisP b2306 0.26 -0.1
2,421,971 - hisP b2306 0.28 -1.7
2,421,973 + hisP b2306 0.28 +1.9
2,421,981 - hisP b2306 0.29 -0.1
2,421,981 - hisP b2306 0.29 -0.7
2,422,005 - hisP b2306 0.32 -2.4
2,422,199 + hisP b2306 0.57 -0.1
2,422,199 + hisP b2306 0.57 -0.1
2,422,218 - hisP b2306 0.59 +0.8
2,422,232 + hisP b2306 0.61 -1.1
2,422,232 + hisP b2306 0.61 -1.1
2,422,255 - hisP b2306 0.64 -0.1
2,422,255 - hisP b2306 0.64 -1.1
2,422,375 + hisP b2306 0.80 -0.1
2,422,486 - -1.1
2,422,538 - +1.1
2,422,538 - -1.1

Or see this region's nucleotide sequence