Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfaX and yfaY are separated by 219 nucleotides yfaY and b2250 are separated by 99 nucleotides b2250 and yfaO are separated by 278 nucleotides
b2248: yfaX - predicted DNA-binding transcriptional regulator (NCBI), at 2,359,451 to 2,360,233
yfaX
b2249: yfaY - competence damage-inducible protein A (NCBI), at 2,360,453 to 2,361,655
yfaY
b2250: b2250 - orf, hypothetical protein (VIMSS), at 2,361,755 to 2,362,297
b2250
b2251: yfaO - predicted NUDIX hydrolase (NCBI), at 2,362,576 to 2,363,001
yfaO
Position (kb)
2360
2361
2362 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2359.482 kb on - strand at 2359.514 kb on + strand at 2359.514 kb on + strand at 2359.686 kb on - strand, within yfaX at 2359.778 kb on + strand, within yfaX at 2359.778 kb on + strand, within yfaX at 2359.907 kb on + strand, within yfaX at 2360.320 kb on - strand at 2360.320 kb on - strand at 2360.432 kb on - strand at 2360.440 kb on - strand at 2360.442 kb on + strand at 2360.465 kb on + strand at 2360.490 kb on - strand at 2360.490 kb on - strand at 2360.571 kb on + strand at 2360.576 kb on + strand, within yfaY at 2360.706 kb on + strand, within yfaY at 2360.855 kb on - strand, within yfaY at 2360.855 kb on - strand, within yfaY at 2360.896 kb on + strand, within yfaY at 2361.049 kb on + strand, within yfaY at 2361.057 kb on - strand, within yfaY at 2361.100 kb on - strand, within yfaY at 2361.100 kb on - strand, within yfaY at 2361.200 kb on + strand, within yfaY at 2361.257 kb on + strand, within yfaY at 2361.301 kb on + strand, within yfaY at 2361.512 kb on + strand, within yfaY at 2361.512 kb on + strand, within yfaY at 2361.518 kb on - strand, within yfaY at 2361.518 kb on - strand, within yfaY at 2361.596 kb on - strand at 2361.712 kb on + strand at 2361.815 kb on + strand, within b2250 at 2361.860 kb on + strand, within b2250 at 2361.869 kb on - strand, within b2250 at 2361.869 kb on - strand, within b2250 at 2361.926 kb on - strand, within b2250 at 2362.040 kb on + strand, within b2250 at 2362.209 kb on + strand, within b2250 at 2362.214 kb on + strand, within b2250 at 2362.221 kb on + strand, within b2250 at 2362.239 kb on - strand, within b2250 at 2362.244 kb on - strand at 2362.244 kb on - strand at 2362.246 kb on + strand at 2362.307 kb on + strand at 2362.307 kb on + strand at 2362.316 kb on - strand at 2362.345 kb on + strand at 2362.345 kb on + strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.351 kb on - strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.353 kb on + strand at 2362.357 kb on + strand at 2362.361 kb on + strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.361 kb on - strand at 2362.365 kb on - strand at 2362.366 kb on + strand at 2362.366 kb on + strand at 2362.399 kb on + strand at 2362.475 kb on + strand at 2362.486 kb on - strand at 2362.488 kb on - strand at 2362.488 kb on - strand at 2362.528 kb on + strand at 2362.531 kb on - strand at 2362.533 kb on + strand at 2362.534 kb on + strand at 2362.534 kb on + strand at 2362.541 kb on - strand at 2362.586 kb on + strand at 2362.590 kb on - strand at 2362.590 kb on - strand at 2362.591 kb on + strand at 2362.599 kb on - strand at 2362.599 kb on - strand at 2362.600 kb on - strand at 2362.600 kb on - strand at 2362.601 kb on - strand at 2362.601 kb on - strand at 2362.634 kb on + strand, within yfaO
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 2,359,482 - +2.2 2,359,514 + +0.9 2,359,514 + -1.7 2,359,686 - yfaX b2248 0.30 +3.7 2,359,778 + yfaX b2248 0.42 -0.1 2,359,778 + yfaX b2248 0.42 -0.1 2,359,907 + yfaX b2248 0.58 +1.1 2,360,320 - +0.9 2,360,320 - +1.2 2,360,432 - -0.8 2,360,440 - +1.1 2,360,442 + -0.1 2,360,465 + -2.1 2,360,490 - -1.7 2,360,490 - -0.1 2,360,571 + -1.4 2,360,576 + yfaY b2249 0.10 -0.9 2,360,706 + yfaY b2249 0.21 -2.1 2,360,855 - yfaY b2249 0.33 -0.1 2,360,855 - yfaY b2249 0.33 -1.1 2,360,896 + yfaY b2249 0.37 -0.1 2,361,049 + yfaY b2249 0.50 -0.1 2,361,057 - yfaY b2249 0.50 -0.3 2,361,100 - yfaY b2249 0.54 -2.2 2,361,100 - yfaY b2249 0.54 -0.6 2,361,200 + yfaY b2249 0.62 +0.2 2,361,257 + yfaY b2249 0.67 -0.1 2,361,301 + yfaY b2249 0.70 -0.5 2,361,512 + yfaY b2249 0.88 +1.5 2,361,512 + yfaY b2249 0.88 -0.1 2,361,518 - yfaY b2249 0.89 -0.4 2,361,518 - yfaY b2249 0.89 -0.5 2,361,596 - -1.5 2,361,712 + +0.3 2,361,815 + b2250 b2250 0.11 +1.9 2,361,860 + b2250 b2250 0.19 -0.5 2,361,869 - b2250 b2250 0.21 -0.1 2,361,869 - b2250 b2250 0.21 -1.1 2,361,926 - b2250 b2250 0.31 -0.3 2,362,040 + b2250 b2250 0.52 +0.6 2,362,209 + b2250 b2250 0.84 +1.0 2,362,214 + b2250 b2250 0.85 +0.7 2,362,221 + b2250 b2250 0.86 -1.1 2,362,239 - b2250 b2250 0.89 -0.3 2,362,244 - +2.1 2,362,244 - -3.2 2,362,246 + -0.7 2,362,307 + +0.7 2,362,307 + +0.7 2,362,316 - -0.3 2,362,345 + +0.1 2,362,345 + -0.1 2,362,351 - +0.6 2,362,351 - +0.2 2,362,351 - +0.4 2,362,351 - -1.0 2,362,351 - -0.4 2,362,351 - +0.5 2,362,351 - +1.9 2,362,351 - -1.4 2,362,351 - -0.3 2,362,351 - -0.8 2,362,353 + +0.6 2,362,353 + +0.0 2,362,353 + -1.1 2,362,353 + +2.1 2,362,353 + +0.2 2,362,353 + +0.1 2,362,357 + -0.7 2,362,361 + -0.5 2,362,361 - -0.7 2,362,361 - +1.1 2,362,361 - +0.9 2,362,361 - -2.4 2,362,361 - +0.2 2,362,361 - -0.3 2,362,361 - +0.9 2,362,365 - -0.5 2,362,366 + -0.1 2,362,366 + -1.1 2,362,399 + +0.2 2,362,475 + +1.3 2,362,486 - -0.5 2,362,488 - +1.5 2,362,488 - +1.5 2,362,528 + +0.8 2,362,531 - +0.8 2,362,533 + -1.4 2,362,534 + -2.1 2,362,534 + +1.2 2,362,541 - -1.7 2,362,586 + +0.7 2,362,590 - +0.5 2,362,590 - -0.3 2,362,591 + -0.1 2,362,599 - -0.1 2,362,599 - -1.7 2,362,600 - +1.2 2,362,600 - +0.5 2,362,601 - +0.7 2,362,601 - -0.9 2,362,634 + yfaO b2251 0.14 +1.4
Or see this region's nucleotide sequence