Strain Fitness in Escherichia coli BW25113 around b2206

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnapH and napG overlap by 14 nucleotidesnapG and napA are separated by 6 nucleotidesnapA and napD overlap by 4 nucleotidesnapD and napF overlap by 11 nucleotidesnapF and yojO are separated by 108 nucleotides b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600 napH b2205: napG - quinol dehydrogenase periplasmic component (NCBI), at 2,297,587 to 2,298,282 napG b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775 napA b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035 napD b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519 napF b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792 yojO Position (kb) 2298 2299 2300 2301Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2297.341 kb on + strand, within napHat 2297.444 kb on - strand, within napHat 2297.464 kb on + strand, within napHat 2297.476 kb on - strand, within napHat 2297.476 kb on - strand, within napHat 2297.517 kb on + strandat 2297.535 kb on - strandat 2297.535 kb on - strandat 2297.585 kb on - strandat 2297.585 kb on - strandat 2297.615 kb on - strandat 2297.676 kb on - strand, within napGat 2297.791 kb on + strand, within napGat 2297.798 kb on + strand, within napGat 2297.949 kb on + strand, within napGat 2297.953 kb on - strand, within napGat 2298.068 kb on + strand, within napGat 2298.073 kb on + strand, within napGat 2298.161 kb on - strand, within napGat 2298.190 kb on + strand, within napGat 2298.194 kb on + strand, within napGat 2298.340 kb on - strandat 2298.340 kb on - strandat 2298.469 kb on + strandat 2298.562 kb on + strand, within napAat 2298.565 kb on + strand, within napAat 2298.570 kb on - strand, within napAat 2298.572 kb on + strand, within napAat 2298.578 kb on - strand, within napAat 2298.582 kb on + strand, within napAat 2298.582 kb on + strand, within napAat 2298.604 kb on - strand, within napAat 2298.604 kb on - strand, within napAat 2298.790 kb on + strand, within napAat 2298.882 kb on - strand, within napAat 2298.981 kb on + strand, within napAat 2298.981 kb on + strand, within napAat 2298.983 kb on + strand, within napAat 2298.983 kb on + strand, within napAat 2299.128 kb on + strand, within napAat 2299.128 kb on + strand, within napAat 2299.139 kb on + strand, within napAat 2299.195 kb on + strand, within napAat 2299.195 kb on + strand, within napAat 2299.267 kb on - strand, within napAat 2299.299 kb on + strand, within napAat 2299.299 kb on + strand, within napAat 2299.342 kb on - strand, within napAat 2299.381 kb on + strand, within napAat 2299.467 kb on + strand, within napAat 2299.503 kb on + strand, within napAat 2299.503 kb on + strand, within napAat 2299.519 kb on - strand, within napAat 2299.519 kb on - strand, within napAat 2299.679 kb on + strand, within napAat 2299.697 kb on + strand, within napAat 2299.697 kb on + strand, within napAat 2299.817 kb on - strand, within napAat 2299.859 kb on + strand, within napAat 2300.072 kb on + strand, within napAat 2300.072 kb on + strand, within napAat 2300.114 kb on + strand, within napAat 2300.239 kb on + strand, within napAat 2300.240 kb on - strand, within napAat 2300.245 kb on - strand, within napAat 2300.338 kb on + strand, within napAat 2300.365 kb on + strand, within napAat 2300.365 kb on + strand, within napAat 2300.421 kb on - strand, within napAat 2300.421 kb on - strand, within napAat 2300.605 kb on + strandat 2300.832 kb on + strand, within napDat 2300.841 kb on + strand, within napDat 2300.865 kb on - strand, within napDat 2300.965 kb on + strand, within napDat 2301.037 kb on + strandat 2301.037 kb on + strandat 2301.060 kb on + strandat 2301.060 kb on + strandat 2301.084 kb on + strand, within napFat 2301.183 kb on - strand, within napFat 2301.210 kb on - strand, within napFat 2301.210 kb on - strand, within napFat 2301.256 kb on - strand, within napFat 2301.257 kb on - strand, within napFat 2301.286 kb on + strand, within napFat 2301.415 kb on - strand, within napFat 2301.496 kb on - strandat 2301.620 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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2,297,341 + napH b2204 0.70 -0.1
2,297,444 - napH b2204 0.82 +1.4
2,297,464 + napH b2204 0.84 +0.2
2,297,476 - napH b2204 0.86 +0.9
2,297,476 - napH b2204 0.86 +1.1
2,297,517 + -2.7
2,297,535 - +2.5
2,297,535 - +3.7
2,297,585 - +1.9
2,297,585 - +0.6
2,297,615 - +2.2
2,297,676 - napG b2205 0.13 +2.1
2,297,791 + napG b2205 0.29 -0.1
2,297,798 + napG b2205 0.30 +1.1
2,297,949 + napG b2205 0.52 +1.5
2,297,953 - napG b2205 0.53 +2.4
2,298,068 + napG b2205 0.69 -0.7
2,298,073 + napG b2205 0.70 +0.3
2,298,161 - napG b2205 0.82 +1.7
2,298,190 + napG b2205 0.87 +0.9
2,298,194 + napG b2205 0.87 +1.5
2,298,340 - +0.5
2,298,340 - +0.2
2,298,469 + +0.1
2,298,562 + napA b2206 0.11 +0.6
2,298,565 + napA b2206 0.11 -0.1
2,298,570 - napA b2206 0.11 +0.4
2,298,572 + napA b2206 0.11 +0.9
2,298,578 - napA b2206 0.12 +1.8
2,298,582 + napA b2206 0.12 +0.7
2,298,582 + napA b2206 0.12 +0.2
2,298,604 - napA b2206 0.13 +0.1
2,298,604 - napA b2206 0.13 -0.9
2,298,790 + napA b2206 0.20 -2.1
2,298,882 - napA b2206 0.24 +0.8
2,298,981 + napA b2206 0.28 +0.3
2,298,981 + napA b2206 0.28 -0.3
2,298,983 + napA b2206 0.28 -0.4
2,298,983 + napA b2206 0.28 +1.5
2,299,128 + napA b2206 0.34 +1.7
2,299,128 + napA b2206 0.34 -2.4
2,299,139 + napA b2206 0.34 -0.1
2,299,195 + napA b2206 0.36 -0.6
2,299,195 + napA b2206 0.36 -0.8
2,299,267 - napA b2206 0.39 +1.1
2,299,299 + napA b2206 0.41 +1.3
2,299,299 + napA b2206 0.41 +1.2
2,299,342 - napA b2206 0.42 +0.5
2,299,381 + napA b2206 0.44 -0.4
2,299,467 + napA b2206 0.47 -0.4
2,299,503 + napA b2206 0.49 +0.7
2,299,503 + napA b2206 0.49 -0.4
2,299,519 - napA b2206 0.49 -0.7
2,299,519 - napA b2206 0.49 -0.8
2,299,679 + napA b2206 0.56 +0.9
2,299,697 + napA b2206 0.57 -2.1
2,299,697 + napA b2206 0.57 +0.5
2,299,817 - napA b2206 0.61 +0.9
2,299,859 + napA b2206 0.63 +0.9
2,300,072 + napA b2206 0.72 -0.5
2,300,072 + napA b2206 0.72 -0.1
2,300,114 + napA b2206 0.73 +0.4
2,300,239 + napA b2206 0.78 +0.2
2,300,240 - napA b2206 0.78 +0.1
2,300,245 - napA b2206 0.79 +0.5
2,300,338 + napA b2206 0.82 -0.5
2,300,365 + napA b2206 0.83 -1.1
2,300,365 + napA b2206 0.83 -0.1
2,300,421 - napA b2206 0.86 -1.7
2,300,421 - napA b2206 0.86 +1.2
2,300,605 + -2.4
2,300,832 + napD b2207 0.23 +0.3
2,300,841 + napD b2207 0.26 +0.2
2,300,865 - napD b2207 0.35 +1.4
2,300,965 + napD b2207 0.73 +0.3
2,301,037 + -0.5
2,301,037 + -1.1
2,301,060 + -0.4
2,301,060 + -0.1
2,301,084 + napF b2208 0.12 -0.4
2,301,183 - napF b2208 0.32 +0.7
2,301,210 - napF b2208 0.37 +2.2
2,301,210 - napF b2208 0.37 +0.9
2,301,256 - napF b2208 0.47 +0.7
2,301,257 - napF b2208 0.47 -0.4
2,301,286 + napF b2208 0.53 +0.9
2,301,415 - napF b2208 0.79 +1.4
2,301,496 - +1.0
2,301,620 + -0.1

Or see this region's nucleotide sequence