Strain Fitness in Escherichia coli BW25113 around b2073

Experiment: Cephalothin 0.01 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegJ and yegK are separated by 64 nucleotidesyegK and yegL overlap by 4 nucleotidesyegL and ryeC are separated by 180 nucleotidesryeC and ibsA overlap by 103 nucleotidesibsA and ryeD are separated by 235 nucleotidesryeD and ibsB overlap by 99 nucleotidesibsB and b2074 are separated by 278 nucleotides b2071: yegJ - hypothetical protein (NCBI), at 2,149,209 to 2,149,670 yegJ b2072: yegK - hypothetical protein (NCBI), at 2,149,735 to 2,150,496 yegK b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152 yegL b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475 ryeC b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432 ibsA b4437: ryeD - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 2,151,668 to 2,151,803 ryeD b4668: ibsB - toxic membrane protein (RefSeq), at 2,151,705 to 2,151,761 ibsB b2074: b2074 - putative membrane protein (VIMSS), at 2,152,040 to 2,153,287 b2074 Position (kb) 2150 2151 2152Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2149.575 kb on - strand, within yegJat 2149.632 kb on - strandat 2149.660 kb on - strandat 2149.660 kb on - strandat 2149.757 kb on - strandat 2149.757 kb on - strandat 2149.776 kb on - strandat 2149.776 kb on - strandat 2149.901 kb on + strand, within yegKat 2149.909 kb on - strand, within yegKat 2149.909 kb on - strand, within yegKat 2149.973 kb on + strand, within yegKat 2149.981 kb on - strand, within yegKat 2150.081 kb on - strand, within yegKat 2150.081 kb on - strand, within yegKat 2150.299 kb on - strand, within yegKat 2150.330 kb on + strand, within yegKat 2150.330 kb on + strand, within yegKat 2150.338 kb on - strand, within yegKat 2150.633 kb on + strand, within yegLat 2150.650 kb on + strand, within yegLat 2150.753 kb on + strand, within yegLat 2150.819 kb on - strand, within yegLat 2150.819 kb on - strand, within yegLat 2150.836 kb on + strand, within yegLat 2150.836 kb on + strand, within yegLat 2150.846 kb on - strand, within yegLat 2150.846 kb on - strand, within yegLat 2150.856 kb on + strand, within yegLat 2150.863 kb on + strand, within yegLat 2150.865 kb on + strand, within yegLat 2150.891 kb on - strand, within yegLat 2150.919 kb on + strand, within yegLat 2150.919 kb on + strand, within yegLat 2150.943 kb on - strand, within yegLat 2150.943 kb on - strand, within yegLat 2150.962 kb on - strand, within yegLat 2151.035 kb on + strand, within yegLat 2151.046 kb on - strand, within yegLat 2151.104 kb on - strandat 2151.104 kb on - strandat 2151.195 kb on - strandat 2151.277 kb on + strandat 2151.299 kb on - strandat 2151.311 kb on - strandat 2151.339 kb on + strandat 2151.339 kb on - strandat 2151.354 kb on + strand, within ryeCat 2151.354 kb on + strand, within ryeCat 2151.388 kb on - strandat 2151.411 kb on + strandat 2151.411 kb on + strandat 2151.432 kb on - strandat 2151.512 kb on - strandat 2151.671 kb on + strandat 2151.747 kb on + strandat 2151.747 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml
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2,149,575 - yegJ b2071 0.79 +0.8
2,149,632 - +0.7
2,149,660 - -1.2
2,149,660 - -1.2
2,149,757 - +0.1
2,149,757 - -1.2
2,149,776 - -1.2
2,149,776 - +0.3
2,149,901 + yegK b2072 0.22 +0.5
2,149,909 - yegK b2072 0.23 +0.1
2,149,909 - yegK b2072 0.23 +1.3
2,149,973 + yegK b2072 0.31 +1.0
2,149,981 - yegK b2072 0.32 +1.2
2,150,081 - yegK b2072 0.45 +1.3
2,150,081 - yegK b2072 0.45 -0.0
2,150,299 - yegK b2072 0.74 +0.6
2,150,330 + yegK b2072 0.78 -1.7
2,150,330 + yegK b2072 0.78 -1.2
2,150,338 - yegK b2072 0.79 -1.3
2,150,633 + yegL b2073 0.21 -1.5
2,150,650 + yegL b2073 0.24 -2.5
2,150,753 + yegL b2073 0.39 -0.4
2,150,819 - yegL b2073 0.49 -0.1
2,150,819 - yegL b2073 0.49 -0.7
2,150,836 + yegL b2073 0.52 -0.8
2,150,836 + yegL b2073 0.52 +0.8
2,150,846 - yegL b2073 0.53 -0.3
2,150,846 - yegL b2073 0.53 -0.2
2,150,856 + yegL b2073 0.55 -0.2
2,150,863 + yegL b2073 0.56 -1.2
2,150,865 + yegL b2073 0.56 +1.4
2,150,891 - yegL b2073 0.60 -0.7
2,150,919 + yegL b2073 0.65 -0.2
2,150,919 + yegL b2073 0.65 -1.2
2,150,943 - yegL b2073 0.68 +0.7
2,150,943 - yegL b2073 0.68 -0.4
2,150,962 - yegL b2073 0.71 -2.0
2,151,035 + yegL b2073 0.82 -0.2
2,151,046 - yegL b2073 0.84 -2.0
2,151,104 - -3.0
2,151,104 - -0.7
2,151,195 - -0.7
2,151,277 + -0.6
2,151,299 - +1.4
2,151,311 - +2.4
2,151,339 + -0.2
2,151,339 - +2.2
2,151,354 + ryeC b4436 0.15 +1.3
2,151,354 + ryeC b4436 0.15 +0.4
2,151,388 - +0.8
2,151,411 + -0.7
2,151,411 + +1.6
2,151,432 - +2.2
2,151,512 - +1.4
2,151,671 + -0.9
2,151,747 + -1.0
2,151,747 + +0.7

Or see this region's nucleotide sequence