Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheB and cheR are separated by 2 nucleotides cheR and tap are separated by 18 nucleotides tap and tar are separated by 45 nucleotides
b1883: cheB - chemotaxis-specific methylesterase (NCBI), at 1,965,476 to 1,966,525
cheB
b1884: cheR - chemotaxis regulator, protein-glutamate methyltransferase (NCBI), at 1,966,528 to 1,967,388
cheR
b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008
tap
b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715
tar
Position (kb)
1967
1968
1969
1970 Strain fitness (log2 ratio)
-2
-1
0
1 at 1966.514 kb on + strand at 1966.553 kb on - strand at 1966.628 kb on + strand, within cheR at 1966.628 kb on + strand, within cheR at 1966.690 kb on - strand, within cheR at 1966.733 kb on + strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.890 kb on - strand, within cheR at 1967.084 kb on - strand, within cheR at 1967.202 kb on + strand, within cheR at 1967.413 kb on - strand at 1967.413 kb on - strand at 1967.576 kb on - strand, within tap at 1967.731 kb on + strand, within tap at 1967.997 kb on - strand, within tap at 1968.203 kb on + strand, within tap at 1968.218 kb on + strand, within tap at 1968.238 kb on - strand, within tap at 1968.316 kb on + strand, within tap at 1968.329 kb on - strand, within tap at 1968.571 kb on - strand, within tap at 1968.651 kb on + strand, within tap at 1968.651 kb on + strand, within tap at 1968.660 kb on + strand, within tap at 1968.786 kb on - strand, within tap at 1968.786 kb on - strand, within tap at 1968.828 kb on - strand, within tap at 1968.852 kb on + strand at 1969.099 kb on + strand at 1969.099 kb on + strand at 1969.425 kb on - strand, within tar at 1969.705 kb on + strand, within tar at 1969.705 kb on + strand, within tar at 1969.713 kb on - strand, within tar at 1969.833 kb on + strand, within tar at 1969.925 kb on - strand, within tar at 1969.957 kb on + strand, within tar
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 1,966,514 + +0.1 1,966,553 - +1.3 1,966,628 + cheR b1884 0.12 -1.8 1,966,628 + cheR b1884 0.12 +0.3 1,966,690 - cheR b1884 0.19 -1.3 1,966,733 + cheR b1884 0.24 +0.5 1,966,883 - cheR b1884 0.41 +0.3 1,966,883 - cheR b1884 0.41 -2.1 1,966,890 - cheR b1884 0.42 +0.0 1,967,084 - cheR b1884 0.65 +0.7 1,967,202 + cheR b1884 0.78 -1.1 1,967,413 - -0.3 1,967,413 - -1.3 1,967,576 - tap b1885 0.11 -1.3 1,967,731 + tap b1885 0.20 -0.7 1,967,997 - tap b1885 0.37 +1.7 1,968,203 + tap b1885 0.50 -2.6 1,968,218 + tap b1885 0.51 +0.1 1,968,238 - tap b1885 0.52 -2.6 1,968,316 + tap b1885 0.57 -0.3 1,968,329 - tap b1885 0.58 -1.2 1,968,571 - tap b1885 0.73 -1.3 1,968,651 + tap b1885 0.78 +0.1 1,968,651 + tap b1885 0.78 +0.1 1,968,660 + tap b1885 0.78 -0.6 1,968,786 - tap b1885 0.86 -0.0 1,968,786 - tap b1885 0.86 -0.8 1,968,828 - tap b1885 0.89 -1.0 1,968,852 + -0.5 1,969,099 + -2.3 1,969,099 + +0.5 1,969,425 - tar b1886 0.22 -0.4 1,969,705 + tar b1886 0.39 +0.1 1,969,705 + tar b1886 0.39 -0.4 1,969,713 - tar b1886 0.40 +1.7 1,969,833 + tar b1886 0.47 -2.5 1,969,925 - tar b1886 0.52 +0.1 1,969,957 + tar b1886 0.54 +1.4
Or see this region's nucleotide sequence