Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxD and rsxG are separated by 3 nucleotides rsxG and rsxE are separated by 3 nucleotides rsxE and nth overlap by 1 nucleotides
b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224
rsxD
b1631: rsxG - electron transport complex protein RnfG (NCBI), at 1,708,228 to 1,708,848
rsxG
b1632: rsxE - NADH-ubiquinone oxidoreductase (NCBI), at 1,708,852 to 1,709,547
rsxE
b1633: nth - DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI), at 1,709,547 to 1,710,182
nth
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1707.895 kb on + strand, within rsxD at 1707.914 kb on - strand, within rsxD at 1707.924 kb on + strand, within rsxD at 1708.021 kb on + strand, within rsxD at 1708.161 kb on + strand at 1708.161 kb on + strand at 1708.187 kb on - strand at 1708.227 kb on - strand at 1708.253 kb on + strand at 1708.253 kb on + strand at 1708.996 kb on - strand, within rsxE at 1709.004 kb on + strand, within rsxE at 1709.034 kb on - strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.106 kb on - strand, within rsxE at 1709.123 kb on + strand, within rsxE at 1709.128 kb on + strand, within rsxE at 1709.182 kb on + strand, within rsxE at 1709.254 kb on + strand, within rsxE at 1709.303 kb on - strand, within rsxE at 1709.320 kb on + strand, within rsxE at 1709.330 kb on - strand, within rsxE at 1709.330 kb on - strand, within rsxE at 1709.393 kb on + strand, within rsxE at 1709.445 kb on + strand, within rsxE at 1709.464 kb on - strand, within rsxE at 1709.517 kb on - strand at 1709.581 kb on + strand at 1709.581 kb on + strand at 1709.691 kb on - strand, within nth at 1709.760 kb on - strand, within nth at 1709.843 kb on + strand, within nth at 1709.859 kb on + strand, within nth at 1709.859 kb on + strand, within nth at 1709.876 kb on + strand, within nth at 1709.876 kb on + strand, within nth at 1710.159 kb on + strand at 1710.274 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 1,707,895 + rsxD b1630 0.69 -0.5 1,707,914 - rsxD b1630 0.71 +2.3 1,707,924 + rsxD b1630 0.72 +3.0 1,708,021 + rsxD b1630 0.81 +1.5 1,708,161 + +1.8 1,708,161 + +3.7 1,708,187 - +1.1 1,708,227 - +1.0 1,708,253 + +0.5 1,708,253 + +0.0 1,708,996 - rsxE b1632 0.21 +1.5 1,709,004 + rsxE b1632 0.22 +3.2 1,709,034 - rsxE b1632 0.26 +1.5 1,709,096 + rsxE b1632 0.35 +1.5 1,709,096 + rsxE b1632 0.35 +0.6 1,709,106 - rsxE b1632 0.36 +0.7 1,709,123 + rsxE b1632 0.39 +1.0 1,709,128 + rsxE b1632 0.40 +0.5 1,709,182 + rsxE b1632 0.47 +2.0 1,709,254 + rsxE b1632 0.58 +2.4 1,709,303 - rsxE b1632 0.65 +0.5 1,709,320 + rsxE b1632 0.67 +0.9 1,709,330 - rsxE b1632 0.69 +2.0 1,709,330 - rsxE b1632 0.69 +1.2 1,709,393 + rsxE b1632 0.78 +2.9 1,709,445 + rsxE b1632 0.85 +1.9 1,709,464 - rsxE b1632 0.88 -1.1 1,709,517 - +0.9 1,709,581 + +0.0 1,709,581 + +0.5 1,709,691 - nth b1633 0.23 +0.5 1,709,760 - nth b1633 0.33 -2.8 1,709,843 + nth b1633 0.47 +1.5 1,709,859 + nth b1633 0.49 +1.2 1,709,859 + nth b1633 0.49 +0.0 1,709,876 + nth b1633 0.52 -1.5 1,709,876 + nth b1633 0.52 -0.9 1,710,159 + +0.1 1,710,274 - +0.7
Or see this region's nucleotide sequence