Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppD and oppF overlap by 4 nucleotides oppF and b1248 are separated by 52 nucleotides b1248 and cls are separated by 34 nucleotides
b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791
oppD
b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792
oppF
b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174
b1248
b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669
cls
Position (kb)
1303
1304
1305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1302.812 kb on + strand at 1302.812 kb on + strand at 1302.860 kb on + strand at 1302.933 kb on + strand, within oppD at 1303.045 kb on + strand, within oppD at 1303.071 kb on + strand, within oppD at 1303.132 kb on + strand, within oppD at 1303.142 kb on + strand, within oppD at 1303.155 kb on + strand, within oppD at 1303.165 kb on + strand, within oppD at 1303.201 kb on - strand, within oppD at 1303.342 kb on - strand, within oppD at 1303.359 kb on + strand, within oppD at 1303.403 kb on - strand, within oppD at 1303.405 kb on + strand, within oppD at 1303.434 kb on - strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.517 kb on + strand, within oppD at 1303.563 kb on + strand, within oppD at 1303.571 kb on - strand, within oppD at 1303.902 kb on + strand, within oppF at 1303.914 kb on - strand, within oppF at 1303.981 kb on + strand, within oppF at 1303.981 kb on + strand, within oppF at 1304.158 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.367 kb on + strand, within oppF at 1304.720 kb on + strand at 1304.780 kb on - strand at 1304.780 kb on - strand at 1305.078 kb on - strand, within b1248 at 1305.078 kb on - strand, within b1248 at 1305.176 kb on + strand at 1305.186 kb on + strand at 1305.539 kb on + strand, within cls at 1305.539 kb on + strand, within cls at 1305.576 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.625 kb on - strand, within cls at 1305.641 kb on + strand, within cls at 1305.641 kb on + strand, within cls
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 1,302,812 + -2.2 1,302,812 + -0.2 1,302,860 + +0.4 1,302,933 + oppD b1246 0.15 -1.2 1,303,045 + oppD b1246 0.26 -1.0 1,303,071 + oppD b1246 0.29 +0.6 1,303,132 + oppD b1246 0.35 -2.8 1,303,142 + oppD b1246 0.36 +0.8 1,303,155 + oppD b1246 0.37 -0.6 1,303,165 + oppD b1246 0.38 +1.1 1,303,201 - oppD b1246 0.42 -0.4 1,303,342 - oppD b1246 0.56 +0.4 1,303,359 + oppD b1246 0.57 +0.0 1,303,403 - oppD b1246 0.62 -0.8 1,303,405 + oppD b1246 0.62 -2.2 1,303,434 - oppD b1246 0.65 -0.7 1,303,439 + oppD b1246 0.65 -0.9 1,303,439 + oppD b1246 0.65 -2.2 1,303,517 + oppD b1246 0.73 -0.6 1,303,563 + oppD b1246 0.77 -1.5 1,303,571 - oppD b1246 0.78 -0.4 1,303,902 + oppF b1247 0.11 -2.1 1,303,914 - oppF b1247 0.13 -1.0 1,303,981 + oppF b1247 0.19 -1.4 1,303,981 + oppF b1247 0.19 +1.2 1,304,158 + oppF b1247 0.37 -0.2 1,304,294 + oppF b1247 0.50 -2.5 1,304,294 + oppF b1247 0.50 -1.5 1,304,367 + oppF b1247 0.58 -1.2 1,304,720 + +0.4 1,304,780 - -0.5 1,304,780 - -0.8 1,305,078 - b1248 b1248 0.71 -0.2 1,305,078 - b1248 b1248 0.71 -0.2 1,305,176 + +1.0 1,305,186 + -1.5 1,305,539 + cls b1249 0.23 -1.2 1,305,539 + cls b1249 0.23 -0.6 1,305,576 - cls b1249 0.25 +2.1 1,305,588 - cls b1249 0.26 +2.2 1,305,588 - cls b1249 0.26 -0.2 1,305,625 - cls b1249 0.28 +0.1 1,305,641 + cls b1249 0.30 -0.2 1,305,641 + cls b1249 0.30 +0.0
Or see this region's nucleotide sequence