Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaA and hyaB overlap by 4 nucleotides hyaB and hyaC are separated by 18 nucleotides hyaC and hyaD overlap by 4 nucleotides
b0972: hyaA - hydrogenase 1, small subunit (NCBI), at 1,031,362 to 1,032,480
hyaA
b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270
hyaB
b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996
hyaC
b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580
hyaD
Position (kb)
1032
1033
1034
1035 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1031.478 kb on + strand, within hyaA at 1031.511 kb on + strand, within hyaA at 1031.552 kb on + strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1032.036 kb on + strand, within hyaA at 1032.165 kb on + strand, within hyaA at 1032.232 kb on + strand, within hyaA at 1032.241 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.409 kb on - strand at 1032.409 kb on - strand at 1032.419 kb on + strand at 1032.419 kb on + strand at 1032.474 kb on + strand at 1032.474 kb on + strand at 1032.527 kb on + strand at 1032.527 kb on + strand at 1032.721 kb on + strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.884 kb on - strand, within hyaB at 1032.940 kb on - strand, within hyaB at 1032.972 kb on - strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.187 kb on + strand, within hyaB at 1033.214 kb on - strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.392 kb on - strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.517 kb on - strand, within hyaB at 1033.529 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.742 kb on + strand, within hyaB at 1033.875 kb on - strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1033.960 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.059 kb on + strand, within hyaB at 1034.191 kb on + strand at 1034.236 kb on - strand at 1034.252 kb on - strand at 1034.276 kb on + strand at 1034.276 kb on + strand at 1034.319 kb on + strand at 1034.319 kb on + strand at 1034.408 kb on - strand, within hyaC at 1034.577 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.720 kb on - strand, within hyaC at 1034.890 kb on - strand, within hyaC at 1034.895 kb on - strand, within hyaC at 1034.940 kb on + strand at 1034.940 kb on + strand at 1034.945 kb on + strand at 1034.947 kb on - strand at 1035.102 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD at 1035.201 kb on + strand, within hyaD
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 1,031,478 + hyaA b0972 0.10 -0.1 1,031,511 + hyaA b0972 0.13 -0.7 1,031,552 + hyaA b0972 0.17 -0.8 1,031,702 - hyaA b0972 0.30 -0.4 1,031,702 - hyaA b0972 0.30 +0.9 1,031,853 - hyaA b0972 0.44 +0.2 1,031,853 - hyaA b0972 0.44 -0.4 1,032,036 + hyaA b0972 0.60 +0.4 1,032,165 + hyaA b0972 0.72 +0.4 1,032,232 + hyaA b0972 0.78 -2.1 1,032,241 + hyaA b0972 0.79 -1.2 1,032,283 + hyaA b0972 0.82 -1.7 1,032,283 + hyaA b0972 0.82 -0.9 1,032,409 - +1.7 1,032,409 - +0.4 1,032,419 + -0.1 1,032,419 + -0.1 1,032,474 + +0.7 1,032,474 + +0.4 1,032,527 + -0.1 1,032,527 + -0.7 1,032,721 + hyaB b0973 0.14 +1.1 1,032,822 - hyaB b0973 0.19 -0.7 1,032,822 - hyaB b0973 0.19 -0.1 1,032,884 - hyaB b0973 0.23 +1.0 1,032,940 - hyaB b0973 0.26 +1.9 1,032,972 - hyaB b0973 0.28 +0.3 1,033,010 + hyaB b0973 0.30 -1.1 1,033,010 + hyaB b0973 0.30 -0.1 1,033,093 + hyaB b0973 0.34 -0.1 1,033,093 + hyaB b0973 0.34 -1.4 1,033,093 + hyaB b0973 0.34 -1.7 1,033,187 + hyaB b0973 0.40 +2.5 1,033,214 - hyaB b0973 0.41 -0.3 1,033,249 + hyaB b0973 0.43 +0.9 1,033,249 + hyaB b0973 0.43 +0.5 1,033,268 + hyaB b0973 0.44 -0.1 1,033,268 + hyaB b0973 0.44 +2.0 1,033,392 - hyaB b0973 0.51 -1.5 1,033,419 + hyaB b0973 0.53 +0.3 1,033,419 + hyaB b0973 0.53 +0.2 1,033,517 - hyaB b0973 0.58 -1.1 1,033,517 - hyaB b0973 0.58 -0.1 1,033,529 + hyaB b0973 0.59 -0.6 1,033,742 + hyaB b0973 0.71 +4.2 1,033,742 + hyaB b0973 0.71 +5.5 1,033,875 - hyaB b0973 0.78 +0.9 1,033,960 + hyaB b0973 0.83 -0.1 1,033,960 + hyaB b0973 0.83 -0.1 1,034,059 + hyaB b0973 0.88 +0.5 1,034,059 + hyaB b0973 0.88 -0.1 1,034,191 + -0.5 1,034,236 - +0.4 1,034,252 - -1.0 1,034,276 + +3.2 1,034,276 + -2.1 1,034,319 + -1.1 1,034,319 + -3.3 1,034,408 - hyaC b0974 0.17 -0.1 1,034,577 - hyaC b0974 0.41 -0.8 1,034,720 - hyaC b0974 0.61 -0.1 1,034,720 - hyaC b0974 0.61 -1.1 1,034,890 - hyaC b0974 0.85 +1.9 1,034,895 - hyaC b0974 0.86 +0.5 1,034,940 + +0.5 1,034,940 + +0.6 1,034,945 + +0.6 1,034,947 - -0.5 1,035,102 + hyaD b0975 0.19 -0.8 1,035,201 + hyaD b0975 0.35 -0.8 1,035,201 + hyaD b0975 0.35 +2.0
Or see this region's nucleotide sequence