Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhtA and ompX are separated by 352 nucleotides ompX and ybiP are separated by 48 nucleotides ybiP and yliL are separated by 73 nucleotides yliL and rybA are separated by 11 nucleotides rybA and mntR are separated by 142 nucleotides
b0813: rhtA - threonine and homoserine efflux system (NCBI), at 848,433 to 849,320
rhtA
b0814: ompX - outer membrane protein X (NCBI), at 849,673 to 850,188
ompX
b0815: ybiP - predicted hydrolase, inner membrane (NCBI), at 850,237 to 851,820
ybiP
b0816: yliL - hypothetical protein (NCBI), at 851,894 to 852,163
yliL
b4416: rybA - unknown RNA (NCBI), at 852,175 to 852,263
rybA
b0817: mntR - DNA-binding transcriptional regulator of mntH (NCBI), at 852,406 to 852,873
mntR
Position (kb) 850
851
852 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 849.248 kb on + strand at 849.272 kb on + strand at 849.284 kb on - strand at 849.319 kb on - strand at 849.324 kb on - strand at 849.371 kb on - strand at 849.371 kb on - strand at 849.374 kb on + strand at 849.698 kb on - strand at 849.739 kb on - strand, within ompX at 849.809 kb on + strand, within ompX at 849.850 kb on - strand, within ompX at 849.893 kb on + strand, within ompX at 849.902 kb on + strand, within ompX at 849.902 kb on + strand, within ompX at 849.965 kb on + strand, within ompX at 849.990 kb on - strand, within ompX at 850.018 kb on + strand, within ompX at 850.019 kb on + strand, within ompX at 850.027 kb on - strand, within ompX at 850.273 kb on + strand at 850.281 kb on - strand at 850.342 kb on - strand at 850.384 kb on - strand at 850.412 kb on + strand, within ybiP at 850.448 kb on - strand, within ybiP at 850.544 kb on - strand, within ybiP at 850.544 kb on - strand, within ybiP at 850.557 kb on - strand, within ybiP at 850.594 kb on + strand, within ybiP at 850.594 kb on + strand, within ybiP at 850.600 kb on + strand, within ybiP at 850.706 kb on + strand, within ybiP at 850.755 kb on + strand, within ybiP at 850.755 kb on + strand, within ybiP at 851.041 kb on - strand, within ybiP at 851.041 kb on - strand, within ybiP at 851.157 kb on + strand, within ybiP at 851.157 kb on + strand, within ybiP at 851.374 kb on + strand, within ybiP at 851.385 kb on + strand, within ybiP at 851.385 kb on + strand, within ybiP at 851.395 kb on + strand, within ybiP at 851.458 kb on - strand, within ybiP at 851.537 kb on - strand, within ybiP at 851.537 kb on - strand, within ybiP at 851.573 kb on + strand, within ybiP at 851.579 kb on - strand, within ybiP at 851.624 kb on - strand, within ybiP at 851.644 kb on + strand, within ybiP at 851.644 kb on + strand, within ybiP at 851.647 kb on + strand, within ybiP at 851.655 kb on - strand, within ybiP at 851.662 kb on + strand at 851.693 kb on + strand at 851.702 kb on + strand at 851.718 kb on - strand at 851.718 kb on - strand at 851.755 kb on - strand at 851.871 kb on + strand at 851.874 kb on - strand at 851.875 kb on + strand at 851.875 kb on + strand at 851.969 kb on - strand, within yliL at 852.203 kb on + strand, within rybA at 852.479 kb on - strand, within mntR at 852.549 kb on + strand, within mntR at 852.549 kb on + strand, within mntR at 852.561 kb on + strand, within mntR at 852.575 kb on - strand, within mntR at 852.602 kb on - strand, within mntR
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 849,248 + +1.5 849,272 + +1.0 849,284 - -2.0 849,319 - -2.0 849,324 - -1.0 849,371 - -0.3 849,371 - -1.0 849,374 + +0.8 849,698 - -1.8 849,739 - ompX b0814 0.13 -0.0 849,809 + ompX b0814 0.26 -1.0 849,850 - ompX b0814 0.34 -2.0 849,893 + ompX b0814 0.43 -0.0 849,902 + ompX b0814 0.44 -0.0 849,902 + ompX b0814 0.44 -2.4 849,965 + ompX b0814 0.57 -1.6 849,990 - ompX b0814 0.61 -0.0 850,018 + ompX b0814 0.67 -1.0 850,019 + ompX b0814 0.67 +0.3 850,027 - ompX b0814 0.69 -0.5 850,273 + -0.8 850,281 - -0.2 850,342 - -0.0 850,384 - -0.0 850,412 + ybiP b0815 0.11 -0.9 850,448 - ybiP b0815 0.13 -0.8 850,544 - ybiP b0815 0.19 +1.3 850,544 - ybiP b0815 0.19 +2.8 850,557 - ybiP b0815 0.20 -0.0 850,594 + ybiP b0815 0.23 -1.0 850,594 + ybiP b0815 0.23 -1.6 850,600 + ybiP b0815 0.23 -1.0 850,706 + ybiP b0815 0.30 -1.0 850,755 + ybiP b0815 0.33 +1.5 850,755 + ybiP b0815 0.33 -0.0 851,041 - ybiP b0815 0.51 +0.4 851,041 - ybiP b0815 0.51 -0.6 851,157 + ybiP b0815 0.58 -1.0 851,157 + ybiP b0815 0.58 -0.8 851,374 + ybiP b0815 0.72 -1.8 851,385 + ybiP b0815 0.72 -1.6 851,385 + ybiP b0815 0.72 +0.4 851,395 + ybiP b0815 0.73 -0.0 851,458 - ybiP b0815 0.77 -0.6 851,537 - ybiP b0815 0.82 +1.5 851,537 - ybiP b0815 0.82 -0.0 851,573 + ybiP b0815 0.84 -2.0 851,579 - ybiP b0815 0.85 -0.5 851,624 - ybiP b0815 0.88 -0.6 851,644 + ybiP b0815 0.89 -1.5 851,644 + ybiP b0815 0.89 -0.8 851,647 + ybiP b0815 0.89 -0.7 851,655 - ybiP b0815 0.90 -1.2 851,662 + -0.8 851,693 + +0.3 851,702 + -2.4 851,718 - -0.0 851,718 - -0.0 851,755 - -0.5 851,871 + -0.9 851,874 - -0.9 851,875 + +0.1 851,875 + -0.0 851,969 - yliL b0816 0.28 +2.0 852,203 + rybA b4416 0.31 -2.4 852,479 - mntR b0817 0.16 -0.0 852,549 + mntR b0817 0.31 +0.7 852,549 + mntR b0817 0.31 -1.4 852,561 + mntR b0817 0.33 -0.5 852,575 - mntR b0817 0.36 -0.0 852,602 - mntR b0817 0.42 -0.5
Or see this region's nucleotide sequence