Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeX and ybeY are separated by 89 nucleotides ybeY and ybeZ overlap by 4 nucleotides ybeZ and miaB are separated by 152 nucleotides
b0658: ybeX - predicteed ion transport (NCBI), at 690,129 to 691,007
ybeX
b0659: ybeY - hypothetical protein (NCBI), at 691,097 to 691,564
ybeY
b0660: ybeZ - predicted protein with nucleoside triphosphate hydrolase domain (RefSeq), at 691,561 to 692,601
ybeZ
b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178
miaB
Position (kb) 691
692
693 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 690.589 kb on - strand, within ybeX at 690.609 kb on - strand, within ybeX at 690.713 kb on - strand, within ybeX at 690.779 kb on - strand, within ybeX at 690.795 kb on - strand, within ybeX at 690.795 kb on - strand, within ybeX at 690.809 kb on + strand, within ybeX at 690.817 kb on - strand, within ybeX at 691.017 kb on - strand at 691.646 kb on - strand at 691.668 kb on - strand, within ybeZ at 691.757 kb on + strand, within ybeZ at 691.757 kb on + strand, within ybeZ at 691.766 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.842 kb on + strand, within ybeZ at 691.849 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.576 kb on - strand at 692.576 kb on - strand at 692.598 kb on - strand at 692.598 kb on - strand at 692.774 kb on + strand at 692.914 kb on + strand, within miaB at 693.086 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.141 kb on + strand, within miaB at 693.175 kb on + strand, within miaB at 693.175 kb on - strand, within miaB at 693.175 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.236 kb on - strand, within miaB at 693.302 kb on + strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.337 kb on - strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.595 kb on - strand, within miaB
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 690,589 - ybeX b0658 0.52 +2.3 690,609 - ybeX b0658 0.55 -0.4 690,713 - ybeX b0658 0.66 +1.1 690,779 - ybeX b0658 0.74 -0.0 690,795 - ybeX b0658 0.76 +3.0 690,795 - ybeX b0658 0.76 +0.7 690,809 + ybeX b0658 0.77 +1.2 690,817 - ybeX b0658 0.78 +0.9 691,017 - -0.0 691,646 - -1.0 691,668 - ybeZ b0660 0.10 -1.8 691,757 + ybeZ b0660 0.19 -1.0 691,757 + ybeZ b0660 0.19 -0.0 691,766 - ybeZ b0660 0.20 -1.4 691,793 - ybeZ b0660 0.22 -1.0 691,793 - ybeZ b0660 0.22 -1.6 691,842 + ybeZ b0660 0.27 -0.0 691,849 - ybeZ b0660 0.28 -1.6 692,300 - ybeZ b0660 0.71 -2.0 692,300 - ybeZ b0660 0.71 -0.7 692,407 - ybeZ b0660 0.81 +0.4 692,407 - ybeZ b0660 0.81 +0.4 692,576 - -0.4 692,576 - -0.9 692,598 - -1.3 692,598 - -0.6 692,774 + +0.1 692,914 + miaB b0661 0.11 +1.6 693,086 + miaB b0661 0.23 -2.0 693,088 + miaB b0661 0.23 -1.6 693,088 + miaB b0661 0.23 +1.0 693,141 + miaB b0661 0.27 -0.6 693,175 + miaB b0661 0.30 -0.9 693,175 - miaB b0661 0.30 -0.0 693,175 - miaB b0661 0.30 +0.6 693,183 - miaB b0661 0.30 +0.5 693,183 - miaB b0661 0.30 +2.6 693,236 - miaB b0661 0.34 +1.3 693,302 + miaB b0661 0.38 +1.1 693,310 - miaB b0661 0.39 -1.0 693,310 - miaB b0661 0.39 +1.6 693,310 - miaB b0661 0.39 +1.3 693,310 - miaB b0661 0.39 -0.2 693,337 - miaB b0661 0.41 +0.5 693,380 + miaB b0661 0.44 +1.0 693,380 + miaB b0661 0.44 -2.0 693,380 + miaB b0661 0.44 -0.3 693,595 - miaB b0661 0.59 -0.0
Or see this region's nucleotide sequence