Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pauD and envY are separated by 45 nucleotides envY and ybcH are separated by 182 nucleotides ybcH and nfrA are separated by 0 nucleotides
b4635: pauD - tRNA-OTHER (RefSeq), at 585,280 to 585,324
pauD
b0566: envY - DNA-binding transcriptional activator of porin biosynthesis (NCBI), at 585,370 to 586,131
envY
b0567: ybcH - hypothetical protein (NCBI), at 586,314 to 587,204
ybcH
b0568: nfrA - bacteriophage N4 receptor, outer membrane subunit (NCBI), at 587,205 to 590,177
nfrA
Position (kb) 586
587
588 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 585.335 kb on + strand at 585.346 kb on + strand at 585.346 kb on + strand at 585.353 kb on - strand at 585.368 kb on + strand at 585.368 kb on + strand at 585.373 kb on - strand at 585.527 kb on + strand, within envY at 585.527 kb on - strand, within envY at 585.527 kb on - strand, within envY at 585.533 kb on + strand, within envY at 585.533 kb on + strand, within envY at 585.541 kb on - strand, within envY at 585.542 kb on - strand, within envY at 585.566 kb on - strand, within envY at 585.566 kb on - strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.600 kb on + strand, within envY at 585.608 kb on - strand, within envY at 585.608 kb on - strand, within envY at 585.614 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.649 kb on - strand, within envY at 585.685 kb on + strand, within envY at 585.685 kb on + strand, within envY at 585.703 kb on - strand, within envY at 585.715 kb on - strand, within envY at 585.833 kb on + strand, within envY at 585.857 kb on - strand, within envY at 585.963 kb on - strand, within envY at 585.963 kb on - strand, within envY at 585.972 kb on - strand, within envY at 586.074 kb on + strand at 586.123 kb on - strand at 586.150 kb on + strand at 586.305 kb on - strand at 586.312 kb on + strand at 586.312 kb on + strand at 586.528 kb on - strand, within ybcH at 586.541 kb on + strand, within ybcH at 586.541 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.574 kb on + strand, within ybcH at 586.627 kb on - strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.834 kb on - strand, within ybcH at 586.834 kb on - strand, within ybcH at 587.002 kb on - strand, within ybcH at 587.062 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.081 kb on - strand, within ybcH at 587.114 kb on + strand, within ybcH at 587.214 kb on - strand at 587.441 kb on + strand at 587.759 kb on + strand, within nfrA at 587.796 kb on - strand, within nfrA at 587.825 kb on - strand, within nfrA at 587.844 kb on + strand, within nfrA at 587.871 kb on + strand, within nfrA at 587.953 kb on + strand, within nfrA at 588.029 kb on - strand, within nfrA at 588.029 kb on - strand, within nfrA at 588.152 kb on - strand, within nfrA at 588.199 kb on + strand, within nfrA at 588.199 kb on + strand, within nfrA at 588.201 kb on - strand, within nfrA at 588.202 kb on + strand, within nfrA at 588.202 kb on + strand, within nfrA
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 585,335 + +0.0 585,346 + +0.8 585,346 + +1.1 585,353 - +0.8 585,368 + +0.0 585,368 + -0.4 585,373 - -0.1 585,527 + envY b0566 0.21 -1.8 585,527 - envY b0566 0.21 +1.0 585,527 - envY b0566 0.21 -1.3 585,533 + envY b0566 0.21 +0.3 585,533 + envY b0566 0.21 -2.3 585,541 - envY b0566 0.22 +1.0 585,542 - envY b0566 0.23 +0.0 585,566 - envY b0566 0.26 +0.6 585,566 - envY b0566 0.26 -3.1 585,600 + envY b0566 0.30 +0.6 585,600 + envY b0566 0.30 -1.5 585,600 + envY b0566 0.30 +0.2 585,600 + envY b0566 0.30 +2.0 585,608 - envY b0566 0.31 -1.0 585,608 - envY b0566 0.31 -0.3 585,614 - envY b0566 0.32 -0.7 585,649 - envY b0566 0.37 +0.5 585,649 - envY b0566 0.37 +0.0 585,649 - envY b0566 0.37 -0.2 585,685 + envY b0566 0.41 +0.5 585,685 + envY b0566 0.41 +2.0 585,703 - envY b0566 0.44 -1.0 585,715 - envY b0566 0.45 -1.8 585,833 + envY b0566 0.61 +0.0 585,857 - envY b0566 0.64 +0.0 585,963 - envY b0566 0.78 +0.4 585,963 - envY b0566 0.78 -1.2 585,972 - envY b0566 0.79 +2.4 586,074 + -1.0 586,123 - +0.5 586,150 + +0.4 586,305 - +0.1 586,312 + -0.2 586,312 + -0.7 586,528 - ybcH b0567 0.24 -0.4 586,541 + ybcH b0567 0.25 +0.4 586,541 - ybcH b0567 0.25 -0.3 586,544 - ybcH b0567 0.26 -0.1 586,544 - ybcH b0567 0.26 -0.2 586,574 + ybcH b0567 0.29 -1.4 586,627 - ybcH b0567 0.35 -1.0 586,794 + ybcH b0567 0.54 -1.5 586,794 + ybcH b0567 0.54 -0.2 586,834 - ybcH b0567 0.58 +1.6 586,834 - ybcH b0567 0.58 -1.4 587,002 - ybcH b0567 0.77 -0.5 587,062 + ybcH b0567 0.84 -0.3 587,073 + ybcH b0567 0.85 +0.0 587,073 + ybcH b0567 0.85 +0.0 587,081 - ybcH b0567 0.86 -0.2 587,114 + ybcH b0567 0.90 -1.2 587,214 - +0.7 587,441 + +0.5 587,759 + nfrA b0568 0.19 +0.0 587,796 - nfrA b0568 0.20 +0.0 587,825 - nfrA b0568 0.21 +0.9 587,844 + nfrA b0568 0.21 -0.5 587,871 + nfrA b0568 0.22 -0.8 587,953 + nfrA b0568 0.25 -0.8 588,029 - nfrA b0568 0.28 -0.4 588,029 - nfrA b0568 0.28 +1.6 588,152 - nfrA b0568 0.32 -0.5 588,199 + nfrA b0568 0.33 +1.2 588,199 + nfrA b0568 0.33 -1.3 588,201 - nfrA b0568 0.34 +1.4 588,202 + nfrA b0568 0.34 +0.0 588,202 + nfrA b0568 0.34 -0.8
Or see this region's nucleotide sequence