Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proY and malZ are separated by 158 nucleotides malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides
b0402: proY - predicted cryptic proline transporter (NCBI), at 420,210 to 421,583
proY
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
Position (kb) 421
422
423
424 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 420.934 kb on + strand, within proY at 420.944 kb on + strand, within proY at 420.984 kb on - strand, within proY at 421.003 kb on - strand, within proY at 421.036 kb on + strand, within proY at 421.037 kb on - strand, within proY at 421.126 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.152 kb on - strand, within proY at 421.152 kb on - strand, within proY at 421.157 kb on - strand, within proY at 421.197 kb on + strand, within proY at 421.261 kb on - strand, within proY at 421.387 kb on - strand, within proY at 421.456 kb on - strand at 421.468 kb on + strand at 421.479 kb on + strand at 421.507 kb on - strand at 421.592 kb on - strand at 421.592 kb on - strand at 421.611 kb on + strand at 421.668 kb on - strand at 421.668 kb on - strand at 421.752 kb on - strand at 421.752 kb on - strand at 421.809 kb on - strand at 421.862 kb on - strand at 421.890 kb on + strand at 421.951 kb on + strand, within malZ at 421.964 kb on + strand, within malZ at 422.178 kb on - strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.271 kb on - strand, within malZ at 422.291 kb on - strand, within malZ at 422.374 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.494 kb on - strand, within malZ at 422.505 kb on + strand, within malZ at 422.531 kb on - strand, within malZ at 422.539 kb on + strand, within malZ at 422.690 kb on - strand, within malZ at 422.699 kb on + strand, within malZ at 422.745 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.795 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.905 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.968 kb on + strand, within malZ at 423.015 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.071 kb on - strand, within malZ at 423.162 kb on + strand, within malZ at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 420,934 + proY b0402 0.53 -2.2 420,944 + proY b0402 0.53 -0.9 420,984 - proY b0402 0.56 -0.0 421,003 - proY b0402 0.58 -0.9 421,036 + proY b0402 0.60 -0.4 421,037 - proY b0402 0.60 -0.9 421,126 + proY b0402 0.67 +0.7 421,149 + proY b0402 0.68 -2.2 421,149 + proY b0402 0.68 -1.2 421,152 - proY b0402 0.69 -0.4 421,152 - proY b0402 0.69 +0.1 421,157 - proY b0402 0.69 +0.0 421,197 + proY b0402 0.72 -0.1 421,261 - proY b0402 0.76 +1.3 421,387 - proY b0402 0.86 -0.4 421,456 - +0.2 421,468 + -0.6 421,479 + -1.1 421,507 - +1.1 421,592 - +0.4 421,592 - -2.7 421,611 + -1.9 421,668 - -1.5 421,668 - +0.7 421,752 - +0.1 421,752 - -0.5 421,809 - -1.1 421,862 - +0.1 421,890 + -0.5 421,951 + malZ b0403 0.12 +0.3 421,964 + malZ b0403 0.12 -0.2 422,178 - malZ b0403 0.24 +0.2 422,220 + malZ b0403 0.26 +1.1 422,220 + malZ b0403 0.26 +0.9 422,220 + malZ b0403 0.26 +0.1 422,271 - malZ b0403 0.29 +0.1 422,291 - malZ b0403 0.30 -3.0 422,374 - malZ b0403 0.35 -0.9 422,379 - malZ b0403 0.35 +2.9 422,379 - malZ b0403 0.35 +1.7 422,494 - malZ b0403 0.41 -1.1 422,505 + malZ b0403 0.42 -0.3 422,531 - malZ b0403 0.43 +0.6 422,539 + malZ b0403 0.44 -2.7 422,690 - malZ b0403 0.52 +0.8 422,699 + malZ b0403 0.53 -0.1 422,745 + malZ b0403 0.55 -0.5 422,753 + malZ b0403 0.56 -1.7 422,753 + malZ b0403 0.56 +0.1 422,795 - malZ b0403 0.58 +2.1 422,892 - malZ b0403 0.63 -0.3 422,892 - malZ b0403 0.63 +0.4 422,905 + malZ b0403 0.64 +0.1 422,909 + malZ b0403 0.64 -1.9 422,909 + malZ b0403 0.64 +0.7 422,968 + malZ b0403 0.68 -0.1 423,015 - malZ b0403 0.70 +0.5 423,025 - malZ b0403 0.71 +0.1 423,025 - malZ b0403 0.71 +0.7 423,071 - malZ b0403 0.73 -0.5 423,162 + malZ b0403 0.78 -0.5 423,283 - malZ b0403 0.85 +0.1 423,401 - -0.6 423,404 - +1.3 423,404 - -0.7 423,445 - +0.1 423,512 + +2.9 423,548 - -1.2 423,548 - -0.2 423,711 + yajB b0404 0.26 -0.3 423,711 + yajB b0404 0.26 -1.5 423,909 + yajB b0404 0.60 +2.7 423,909 + -0.5 423,923 + yajB b0404 0.62 +0.3 423,996 + yajB b0404 0.75 +0.3 424,011 - yajB b0404 0.77 -1.5 424,112 + +1.1 424,121 - -1.3 424,121 - +0.1 424,232 + +0.8 424,232 + -1.5 424,280 + -0.9 424,280 + +1.5 424,484 - queA b0405 0.23 -0.1 424,484 - queA b0405 0.23 +1.0
Or see this region's nucleotide sequence