Experiment: Cephalothin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fixA and fixB are separated by 14 nucleotides fixB and fixC are separated by 50 nucleotides
b0041: fixA - probable flavoprotein subunit, carnitine metabolism (VIMSS), at 42,403 to 43,173
fixA
b0042: fixB - predicted electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like (NCBI), at 43,188 to 44,129
fixB
b0043: fixC - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 44,180 to 45,466
fixC
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 42.430 kb on - strand at 42.442 kb on - strand at 42.446 kb on + strand at 42.447 kb on - strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.456 kb on + strand at 42.464 kb on - strand at 42.464 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.470 kb on + strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.485 kb on + strand, within fixA at 42.488 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.534 kb on - strand, within fixA at 42.541 kb on + strand, within fixA at 42.550 kb on - strand, within fixA at 42.591 kb on - strand, within fixA at 42.601 kb on - strand, within fixA at 42.606 kb on - strand, within fixA at 42.682 kb on - strand, within fixA at 42.745 kb on + strand, within fixA at 42.873 kb on - strand, within fixA at 43.044 kb on + strand, within fixA at 43.058 kb on + strand, within fixA at 43.087 kb on - strand, within fixA at 43.236 kb on - strand at 43.236 kb on - strand at 43.237 kb on + strand at 43.237 kb on + strand at 43.270 kb on - strand at 43.318 kb on - strand, within fixB at 43.432 kb on + strand, within fixB at 43.506 kb on + strand, within fixB at 43.531 kb on + strand, within fixB at 43.539 kb on - strand, within fixB at 43.574 kb on - strand, within fixB at 43.610 kb on + strand, within fixB at 43.630 kb on - strand, within fixB at 43.630 kb on - strand, within fixB at 43.682 kb on - strand, within fixB at 43.692 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on - strand, within fixB at 43.838 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.871 kb on - strand, within fixB at 43.924 kb on + strand, within fixB at 43.952 kb on + strand, within fixB at 43.970 kb on - strand, within fixB at 44.076 kb on + strand at 44.158 kb on - strand at 44.176 kb on + strand at 44.214 kb on + strand at 44.214 kb on + strand at 44.214 kb on + strand at 44.222 kb on - strand at 44.243 kb on - strand at 44.326 kb on + strand, within fixC at 44.335 kb on - strand, within fixC at 44.335 kb on - strand, within fixC at 44.398 kb on - strand, within fixC at 44.581 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.622 kb on - strand, within fixC at 44.649 kb on + strand, within fixC at 44.675 kb on - strand, within fixC at 44.676 kb on - strand, within fixC at 44.765 kb on + strand, within fixC at 44.857 kb on - strand, within fixC at 44.862 kb on - strand, within fixC at 44.906 kb on - strand, within fixC at 45.034 kb on + strand, within fixC at 45.034 kb on + strand, within fixC at 45.044 kb on + strand, within fixC at 45.044 kb on + strand, within fixC
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.01 mg/ml remove 42,430 - -0.5 42,442 - +0.8 42,446 + +0.5 42,447 - -0.5 42,452 - -0.4 42,452 - +0.1 42,456 + -1.4 42,464 - +0.5 42,464 - +0.2 42,466 - -1.0 42,466 - -1.2 42,466 - -0.4 42,466 - -1.4 42,466 - +3.2 42,470 + +1.8 42,478 - -0.0 42,478 - -0.0 42,478 - -1.6 42,485 + fixA b0041 0.11 -0.1 42,488 - fixA b0041 0.11 -2.1 42,491 - fixA b0041 0.11 +1.5 42,491 - fixA b0041 0.11 +1.1 42,534 - fixA b0041 0.17 -0.0 42,541 + fixA b0041 0.18 -0.0 42,550 - fixA b0041 0.19 +0.2 42,591 - fixA b0041 0.24 +1.0 42,601 - fixA b0041 0.26 -0.7 42,606 - fixA b0041 0.26 -2.4 42,682 - fixA b0041 0.36 -0.9 42,745 + fixA b0041 0.44 -0.4 42,873 - fixA b0041 0.61 -0.0 43,044 + fixA b0041 0.83 -1.8 43,058 + fixA b0041 0.85 -1.1 43,087 - fixA b0041 0.89 +1.8 43,236 - +0.3 43,236 - +0.3 43,237 + +1.2 43,237 + -0.2 43,270 - +1.0 43,318 - fixB b0042 0.14 +0.2 43,432 + fixB b0042 0.26 -0.4 43,506 + fixB b0042 0.34 +0.2 43,531 + fixB b0042 0.36 -1.1 43,539 - fixB b0042 0.37 +0.2 43,574 - fixB b0042 0.41 -0.8 43,610 + fixB b0042 0.45 -0.8 43,630 - fixB b0042 0.47 +0.2 43,630 - fixB b0042 0.47 -1.4 43,682 - fixB b0042 0.52 +0.1 43,692 + fixB b0042 0.54 -3.2 43,740 + fixB b0042 0.59 +0.5 43,740 + fixB b0042 0.59 -0.8 43,835 + fixB b0042 0.69 -1.1 43,835 + fixB b0042 0.69 -0.5 43,835 - fixB b0042 0.69 -1.2 43,838 + fixB b0042 0.69 +0.8 43,867 + fixB b0042 0.72 -0.0 43,867 + fixB b0042 0.72 -0.2 43,871 - fixB b0042 0.73 -0.7 43,924 + fixB b0042 0.78 -0.1 43,952 + fixB b0042 0.81 -0.6 43,970 - fixB b0042 0.83 +0.2 44,076 + -3.6 44,158 - +0.2 44,176 + +0.4 44,214 + -2.4 44,214 + -0.8 44,214 + +0.6 44,222 - -0.1 44,243 - -1.8 44,326 + fixC b0043 0.11 -0.4 44,335 - fixC b0043 0.12 -0.6 44,335 - fixC b0043 0.12 -0.4 44,398 - fixC b0043 0.17 +1.2 44,581 - fixC b0043 0.31 +1.5 44,622 - fixC b0043 0.34 +0.8 44,622 - fixC b0043 0.34 -1.8 44,649 + fixC b0043 0.36 +0.5 44,675 - fixC b0043 0.38 -0.1 44,676 - fixC b0043 0.39 +0.2 44,765 + fixC b0043 0.45 -1.8 44,857 - fixC b0043 0.53 -0.3 44,862 - fixC b0043 0.53 +0.5 44,906 - fixC b0043 0.56 -1.4 45,034 + fixC b0043 0.66 +1.0 45,034 + fixC b0043 0.66 +0.2 45,044 + fixC b0043 0.67 -1.8 45,044 + fixC b0043 0.67 -1.6
Or see this region's nucleotide sequence