Experiment: Malonic (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_00527 and PFR28_00528 are separated by 163 nucleotides PFR28_00528 and PFR28_00529 are separated by 109 nucleotides PFR28_00529 and PFR28_00530 are separated by 73 nucleotides
PFR28_00527: PFR28_00527 - N-(2-amino-2-carboxyethyl)-L-glutamate synthase, at 561,539 to 562,633
_00527
PFR28_00528: PFR28_00528 - hypothetical protein, at 562,797 to 564,410
_00528
PFR28_00529: PFR28_00529 - hypothetical protein, at 564,520 to 565,197
_00529
PFR28_00530: PFR28_00530 - hypothetical protein, at 565,271 to 565,870
_00530
Position (kb) 562
563
564
565 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 561.809 kb on + strand, within PFR28_00527 at 561.809 kb on + strand, within PFR28_00527 at 561.809 kb on + strand, within PFR28_00527 at 561.809 kb on + strand, within PFR28_00527 at 561.893 kb on + strand, within PFR28_00527 at 561.893 kb on + strand, within PFR28_00527 at 561.893 kb on + strand, within PFR28_00527 at 561.925 kb on + strand, within PFR28_00527 at 561.925 kb on + strand, within PFR28_00527 at 561.926 kb on - strand, within PFR28_00527 at 561.926 kb on - strand, within PFR28_00527 at 561.926 kb on - strand, within PFR28_00527 at 561.935 kb on + strand, within PFR28_00527 at 561.935 kb on + strand, within PFR28_00527 at 561.936 kb on - strand, within PFR28_00527 at 561.936 kb on - strand, within PFR28_00527 at 561.995 kb on + strand, within PFR28_00527 at 561.995 kb on + strand, within PFR28_00527 at 562.027 kb on + strand, within PFR28_00527 at 562.028 kb on - strand, within PFR28_00527 at 562.140 kb on - strand, within PFR28_00527 at 562.140 kb on - strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.143 kb on + strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.144 kb on - strand, within PFR28_00527 at 562.358 kb on + strand, within PFR28_00527 at 562.358 kb on + strand, within PFR28_00527 at 562.359 kb on - strand, within PFR28_00527 at 562.386 kb on + strand, within PFR28_00527 at 562.387 kb on - strand, within PFR28_00527 at 562.499 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.511 kb on + strand, within PFR28_00527 at 562.512 kb on - strand, within PFR28_00527 at 562.512 kb on - strand, within PFR28_00527 at 562.512 kb on - strand, within PFR28_00527 at 562.512 kb on - strand, within PFR28_00527 at 562.512 kb on - strand, within PFR28_00527 at 562.514 kb on + strand, within PFR28_00527 at 562.514 kb on + strand, within PFR28_00527 at 562.515 kb on - strand, within PFR28_00527 at 562.515 kb on - strand, within PFR28_00527 at 562.515 kb on - strand, within PFR28_00527 at 562.544 kb on + strand at 562.627 kb on + strand at 562.628 kb on - strand at 562.739 kb on - strand at 562.745 kb on + strand at 562.745 kb on + strand at 562.745 kb on + strand at 562.745 kb on + strand at 562.745 kb on + strand at 562.746 kb on - strand at 562.746 kb on - strand at 562.746 kb on - strand at 562.970 kb on + strand, within PFR28_00528 at 562.970 kb on + strand, within PFR28_00528 at 562.993 kb on - strand, within PFR28_00528 at 563.116 kb on + strand, within PFR28_00528 at 563.116 kb on + strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.117 kb on - strand, within PFR28_00528 at 563.194 kb on + strand, within PFR28_00528 at 563.194 kb on + strand, within PFR28_00528 at 563.194 kb on + strand, within PFR28_00528 at 563.195 kb on - strand, within PFR28_00528 at 563.195 kb on - strand, within PFR28_00528 at 563.195 kb on - strand, within PFR28_00528 at 563.195 kb on - strand, within PFR28_00528 at 563.272 kb on + strand, within PFR28_00528 at 563.273 kb on - strand, within PFR28_00528 at 563.419 kb on + strand, within PFR28_00528 at 563.419 kb on + strand, within PFR28_00528 at 563.419 kb on + strand, within PFR28_00528 at 563.419 kb on + strand, within PFR28_00528 at 563.420 kb on - strand, within PFR28_00528 at 563.420 kb on - strand, within PFR28_00528 at 563.420 kb on - strand, within PFR28_00528 at 563.458 kb on + strand, within PFR28_00528 at 563.459 kb on - strand, within PFR28_00528 at 563.459 kb on - strand, within PFR28_00528 at 563.459 kb on - strand, within PFR28_00528 at 563.491 kb on + strand, within PFR28_00528 at 563.492 kb on - strand, within PFR28_00528 at 563.492 kb on - strand, within PFR28_00528 at 563.492 kb on - strand, within PFR28_00528 at 563.492 kb on - strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.572 kb on + strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.573 kb on - strand, within PFR28_00528 at 563.574 kb on + strand, within PFR28_00528 at 563.574 kb on + strand, within PFR28_00528 at 563.574 kb on + strand, within PFR28_00528 at 563.574 kb on + strand, within PFR28_00528 at 563.575 kb on - strand, within PFR28_00528 at 563.575 kb on - strand, within PFR28_00528 at 563.575 kb on - strand, within PFR28_00528 at 563.615 kb on + strand, within PFR28_00528 at 563.616 kb on - strand, within PFR28_00528 at 563.616 kb on - strand, within PFR28_00528 at 563.616 kb on - strand, within PFR28_00528 at 563.616 kb on - strand, within PFR28_00528 at 563.616 kb on - strand, within PFR28_00528 at 563.651 kb on + strand, within PFR28_00528 at 563.651 kb on + strand, within PFR28_00528 at 563.651 kb on + strand, within PFR28_00528 at 563.651 kb on + strand, within PFR28_00528 at 563.651 kb on + strand, within PFR28_00528 at 563.652 kb on - strand, within PFR28_00528 at 563.652 kb on - strand, within PFR28_00528 at 563.652 kb on - strand, within PFR28_00528 at 563.652 kb on - strand, within PFR28_00528 at 563.818 kb on + strand, within PFR28_00528 at 563.818 kb on + strand, within PFR28_00528 at 563.819 kb on - strand, within PFR28_00528 at 563.902 kb on + strand, within PFR28_00528 at 564.154 kb on + strand, within PFR28_00528 at 564.154 kb on + strand, within PFR28_00528 at 564.154 kb on + strand, within PFR28_00528 at 564.154 kb on + strand, within PFR28_00528 at 564.155 kb on - strand, within PFR28_00528 at 564.155 kb on - strand, within PFR28_00528 at 564.244 kb on + strand, within PFR28_00528 at 564.244 kb on + strand, within PFR28_00528 at 564.244 kb on + strand, within PFR28_00528 at 564.245 kb on - strand, within PFR28_00528 at 564.245 kb on - strand, within PFR28_00528 at 564.245 kb on - strand, within PFR28_00528 at 564.283 kb on + strand at 564.283 kb on + strand at 564.284 kb on - strand at 564.284 kb on - strand at 564.284 kb on - strand at 564.284 kb on - strand at 564.405 kb on + strand at 564.469 kb on + strand at 564.469 kb on + strand at 564.470 kb on - strand at 564.470 kb on - strand at 564.471 kb on + strand at 564.471 kb on + strand at 564.471 kb on + strand at 564.471 kb on + strand at 564.472 kb on - strand at 564.499 kb on + strand at 564.500 kb on - strand at 564.500 kb on - strand at 564.546 kb on - strand at 564.546 kb on - strand at 564.716 kb on + strand, within PFR28_00529 at 564.716 kb on + strand, within PFR28_00529 at 564.716 kb on + strand, within PFR28_00529 at 564.716 kb on + strand, within PFR28_00529 at 564.870 kb on - strand, within PFR28_00529 at 565.254 kb on + strand at 565.254 kb on + strand at 565.254 kb on + strand at 565.277 kb on + strand at 565.277 kb on + strand at 565.277 kb on + strand at 565.277 kb on + strand at 565.278 kb on - strand at 565.278 kb on - strand at 565.278 kb on - strand at 565.278 kb on - strand at 565.323 kb on + strand at 565.324 kb on - strand at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.380 kb on + strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530 at 565.381 kb on - strand, within PFR28_00530
Per-strain Table
Position Strand Gene LocusTag Fraction Malonic (C) 10 mM remove 561,809 + PFR28_00527 0.25 +1.4 561,809 + PFR28_00527 0.25 +0.3 561,809 + PFR28_00527 0.25 -0.5 561,809 + PFR28_00527 0.25 +0.1 561,893 + PFR28_00527 0.32 -0.2 561,893 + PFR28_00527 0.32 -0.5 561,893 + PFR28_00527 0.32 +0.3 561,925 + PFR28_00527 0.35 +1.1 561,925 + PFR28_00527 0.35 +0.5 561,926 - PFR28_00527 0.35 +1.2 561,926 - PFR28_00527 0.35 -0.1 561,926 - PFR28_00527 0.35 -0.3 561,935 + PFR28_00527 0.36 +0.1 561,935 + PFR28_00527 0.36 +0.0 561,936 - PFR28_00527 0.36 -0.8 561,936 - PFR28_00527 0.36 +0.1 561,995 + PFR28_00527 0.42 +0.8 561,995 + PFR28_00527 0.42 -0.6 562,027 + PFR28_00527 0.45 +0.7 562,028 - PFR28_00527 0.45 +2.4 562,140 - PFR28_00527 0.55 -0.6 562,140 - PFR28_00527 0.55 +0.5 562,143 + PFR28_00527 0.55 +0.4 562,143 + PFR28_00527 0.55 +0.3 562,143 + PFR28_00527 0.55 +0.2 562,143 + PFR28_00527 0.55 -0.4 562,143 + PFR28_00527 0.55 +1.0 562,143 + PFR28_00527 0.55 +0.5 562,143 + PFR28_00527 0.55 +1.5 562,143 + PFR28_00527 0.55 +0.6 562,143 + PFR28_00527 0.55 +0.1 562,143 + PFR28_00527 0.55 -0.9 562,143 + PFR28_00527 0.55 +1.3 562,144 - PFR28_00527 0.55 +1.6 562,144 - PFR28_00527 0.55 +0.4 562,144 - PFR28_00527 0.55 -0.7 562,144 - PFR28_00527 0.55 -1.6 562,144 - PFR28_00527 0.55 +0.8 562,144 - PFR28_00527 0.55 -1.5 562,144 - PFR28_00527 0.55 -1.3 562,144 - PFR28_00527 0.55 +1.0 562,144 - PFR28_00527 0.55 +0.2 562,144 - PFR28_00527 0.55 +0.8 562,144 - PFR28_00527 0.55 +1.0 562,144 - PFR28_00527 0.55 +1.8 562,144 - PFR28_00527 0.55 +0.4 562,358 + PFR28_00527 0.75 +0.5 562,358 + PFR28_00527 0.75 +0.5 562,359 - PFR28_00527 0.75 -1.0 562,386 + PFR28_00527 0.77 -0.0 562,387 - PFR28_00527 0.77 +0.8 562,499 + PFR28_00527 0.88 -0.4 562,511 + PFR28_00527 0.89 +0.6 562,511 + PFR28_00527 0.89 -3.7 562,511 + PFR28_00527 0.89 -1.2 562,511 + PFR28_00527 0.89 +1.7 562,511 + PFR28_00527 0.89 +0.1 562,511 + PFR28_00527 0.89 -0.6 562,512 - PFR28_00527 0.89 -1.0 562,512 - PFR28_00527 0.89 +0.9 562,512 - PFR28_00527 0.89 +0.3 562,512 - PFR28_00527 0.89 +0.2 562,512 - PFR28_00527 0.89 -0.9 562,514 + PFR28_00527 0.89 -3.2 562,514 + PFR28_00527 0.89 -0.3 562,515 - PFR28_00527 0.89 +1.0 562,515 - PFR28_00527 0.89 -1.9 562,515 - PFR28_00527 0.89 -0.1 562,544 + +0.5 562,627 + -0.1 562,628 - -0.0 562,739 - -1.9 562,745 + +0.7 562,745 + +0.5 562,745 + +0.7 562,745 + +1.9 562,745 + +1.5 562,746 - +0.1 562,746 - -0.2 562,746 - +3.1 562,970 + PFR28_00528 0.11 +0.2 562,970 + PFR28_00528 0.11 +0.4 562,993 - PFR28_00528 0.12 -0.3 563,116 + PFR28_00528 0.20 +1.2 563,116 + PFR28_00528 0.20 +2.0 563,117 - PFR28_00528 0.20 -0.0 563,117 - PFR28_00528 0.20 -0.4 563,117 - PFR28_00528 0.20 -1.4 563,117 - PFR28_00528 0.20 +1.6 563,117 - PFR28_00528 0.20 -0.4 563,117 - PFR28_00528 0.20 -0.5 563,194 + PFR28_00528 0.25 +0.0 563,194 + PFR28_00528 0.25 +0.5 563,194 + PFR28_00528 0.25 +0.2 563,195 - PFR28_00528 0.25 +1.0 563,195 - PFR28_00528 0.25 -0.7 563,195 - PFR28_00528 0.25 +0.1 563,195 - PFR28_00528 0.25 +1.1 563,272 + PFR28_00528 0.29 +0.3 563,273 - PFR28_00528 0.29 -1.4 563,419 + PFR28_00528 0.39 +1.4 563,419 + PFR28_00528 0.39 +0.8 563,419 + PFR28_00528 0.39 +1.0 563,419 + PFR28_00528 0.39 +0.5 563,420 - PFR28_00528 0.39 -2.1 563,420 - PFR28_00528 0.39 -0.5 563,420 - PFR28_00528 0.39 +0.9 563,458 + PFR28_00528 0.41 +0.1 563,459 - PFR28_00528 0.41 +1.4 563,459 - PFR28_00528 0.41 +1.9 563,459 - PFR28_00528 0.41 +0.6 563,491 + PFR28_00528 0.43 -0.8 563,492 - PFR28_00528 0.43 +1.2 563,492 - PFR28_00528 0.43 +0.3 563,492 - PFR28_00528 0.43 +0.8 563,492 - PFR28_00528 0.43 +0.4 563,572 + PFR28_00528 0.48 +1.0 563,572 + PFR28_00528 0.48 +1.0 563,572 + PFR28_00528 0.48 +0.9 563,572 + PFR28_00528 0.48 +0.2 563,572 + PFR28_00528 0.48 +0.3 563,572 + PFR28_00528 0.48 +0.8 563,572 + PFR28_00528 0.48 +0.7 563,572 + PFR28_00528 0.48 -0.3 563,572 + PFR28_00528 0.48 -0.3 563,572 + PFR28_00528 0.48 -0.9 563,572 + PFR28_00528 0.48 -2.2 563,572 + PFR28_00528 0.48 +0.4 563,572 + PFR28_00528 0.48 -0.4 563,572 + PFR28_00528 0.48 +1.4 563,572 + PFR28_00528 0.48 +0.8 563,573 - PFR28_00528 0.48 +0.8 563,573 - PFR28_00528 0.48 +0.3 563,573 - PFR28_00528 0.48 -0.0 563,573 - PFR28_00528 0.48 +1.0 563,573 - PFR28_00528 0.48 +0.9 563,573 - PFR28_00528 0.48 -2.4 563,573 - PFR28_00528 0.48 -0.5 563,573 - PFR28_00528 0.48 -1.3 563,573 - PFR28_00528 0.48 -0.2 563,573 - PFR28_00528 0.48 +1.6 563,573 - PFR28_00528 0.48 -0.5 563,574 + PFR28_00528 0.48 -1.1 563,574 + PFR28_00528 0.48 +0.8 563,574 + PFR28_00528 0.48 -0.2 563,574 + PFR28_00528 0.48 -0.3 563,575 - PFR28_00528 0.48 -0.2 563,575 - PFR28_00528 0.48 -0.8 563,575 - PFR28_00528 0.48 -0.1 563,615 + PFR28_00528 0.51 +0.8 563,616 - PFR28_00528 0.51 -0.2 563,616 - PFR28_00528 0.51 -0.5 563,616 - PFR28_00528 0.51 -0.1 563,616 - PFR28_00528 0.51 -0.5 563,616 - PFR28_00528 0.51 +0.1 563,651 + PFR28_00528 0.53 +0.9 563,651 + PFR28_00528 0.53 -0.2 563,651 + PFR28_00528 0.53 +1.3 563,651 + PFR28_00528 0.53 +0.1 563,651 + PFR28_00528 0.53 +1.4 563,652 - PFR28_00528 0.53 -0.5 563,652 - PFR28_00528 0.53 +0.6 563,652 - PFR28_00528 0.53 +0.0 563,652 - PFR28_00528 0.53 +0.1 563,818 + PFR28_00528 0.63 -0.3 563,818 + PFR28_00528 0.63 +1.8 563,819 - PFR28_00528 0.63 -2.1 563,902 + PFR28_00528 0.68 -1.4 564,154 + PFR28_00528 0.84 +0.6 564,154 + PFR28_00528 0.84 -2.1 564,154 + PFR28_00528 0.84 +1.4 564,154 + PFR28_00528 0.84 -0.2 564,155 - PFR28_00528 0.84 +0.4 564,155 - PFR28_00528 0.84 +2.0 564,244 + PFR28_00528 0.90 +1.0 564,244 + PFR28_00528 0.90 +0.7 564,244 + PFR28_00528 0.90 +0.3 564,245 - PFR28_00528 0.90 -0.1 564,245 - PFR28_00528 0.90 +1.0 564,245 - PFR28_00528 0.90 -2.5 564,283 + +0.3 564,283 + +1.1 564,284 - -0.5 564,284 - -2.2 564,284 - +0.3 564,284 - +0.2 564,405 + -2.1 564,469 + -0.1 564,469 + +1.3 564,470 - +0.3 564,470 - -0.2 564,471 + -0.4 564,471 + +0.2 564,471 + +0.2 564,471 + +0.6 564,472 - -1.7 564,499 + -1.1 564,500 - +0.9 564,500 - +0.6 564,546 - -0.8 564,546 - -0.7 564,716 + PFR28_00529 0.29 -0.6 564,716 + PFR28_00529 0.29 -0.5 564,716 + PFR28_00529 0.29 +0.0 564,716 + PFR28_00529 0.29 +0.0 564,870 - PFR28_00529 0.52 -0.8 565,254 + +0.3 565,254 + +1.2 565,254 + +0.2 565,277 + -0.5 565,277 + -1.1 565,277 + +0.7 565,277 + -0.8 565,278 - -1.0 565,278 - +0.1 565,278 - -0.2 565,278 - -0.5 565,323 + -1.0 565,324 - +1.4 565,380 + PFR28_00530 0.18 +0.9 565,380 + PFR28_00530 0.18 +0.1 565,380 + PFR28_00530 0.18 -0.4 565,380 + PFR28_00530 0.18 -0.1 565,380 + PFR28_00530 0.18 +0.8 565,380 + PFR28_00530 0.18 +0.6 565,380 + PFR28_00530 0.18 +1.2 565,380 + PFR28_00530 0.18 +1.8 565,380 + PFR28_00530 0.18 -1.0 565,380 + PFR28_00530 0.18 -0.5 565,380 + PFR28_00530 0.18 +0.1 565,380 + PFR28_00530 0.18 -1.3 565,381 - PFR28_00530 0.18 -1.0 565,381 - PFR28_00530 0.18 +1.6 565,381 - PFR28_00530 0.18 -0.7 565,381 - PFR28_00530 0.18 -0.1 565,381 - PFR28_00530 0.18 -0.4 565,381 - PFR28_00530 0.18 -1.8 565,381 - PFR28_00530 0.18 +0.0 565,381 - PFR28_00530 0.18 -0.1 565,381 - PFR28_00530 0.18 +0.1 565,381 - PFR28_00530 0.18 -1.5
Or see this region's nucleotide sequence