Strain Fitness in Escherichia coli BW25113 around b2054

Experiment: Chloride 750 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgmd and wcaF are separated by 25 nucleotideswcaF and wcaE are separated by 15 nucleotideswcaE and wcaD are separated by 10 nucleotides b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338 gmd b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912 wcaF b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674 wcaE b2056: wcaD - predicted colanic acid polymerase (NCBI), at 2,127,685 to 2,128,902 wcaD Position (kb) 2126 2127Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2125.556 kb on + strand, within gmdat 2125.566 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.570 kb on + strand, within gmdat 2125.643 kb on + strand, within gmdat 2125.706 kb on + strand, within gmdat 2125.707 kb on - strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.797 kb on + strand, within gmdat 2125.828 kb on + strand, within gmdat 2125.887 kb on + strand, within gmdat 2125.941 kb on - strand, within gmdat 2126.048 kb on + strand, within gmdat 2126.090 kb on - strand, within gmdat 2126.093 kb on - strand, within gmdat 2126.151 kb on + strand, within gmdat 2126.181 kb on - strand, within gmdat 2126.225 kb on - strand, within gmdat 2126.225 kb on - strand, within gmdat 2126.247 kb on - strandat 2126.247 kb on - strandat 2126.247 kb on - strandat 2126.267 kb on + strandat 2126.388 kb on + strandat 2126.398 kb on - strandat 2126.451 kb on - strandat 2126.451 kb on - strand, within wcaFat 2126.456 kb on - strand, within wcaFat 2126.456 kb on - strand, within wcaFat 2126.498 kb on + strand, within wcaFat 2126.515 kb on + strand, within wcaFat 2126.550 kb on - strand, within wcaFat 2126.550 kb on - strand, within wcaFat 2126.562 kb on + strand, within wcaFat 2126.562 kb on - strand, within wcaFat 2126.562 kb on - strand, within wcaFat 2126.577 kb on + strand, within wcaFat 2126.577 kb on + strand, within wcaFat 2126.584 kb on - strand, within wcaFat 2126.587 kb on + strand, within wcaFat 2126.614 kb on + strand, within wcaFat 2126.616 kb on + strand, within wcaFat 2126.621 kb on + strand, within wcaFat 2126.629 kb on + strand, within wcaFat 2126.660 kb on - strand, within wcaFat 2126.681 kb on + strand, within wcaFat 2126.681 kb on + strand, within wcaFat 2126.914 kb on + strandat 2127.243 kb on - strand, within wcaEat 2127.467 kb on - strand, within wcaEat 2127.583 kb on + strand, within wcaEat 2127.584 kb on + strand, within wcaEat 2127.617 kb on + strandat 2127.653 kb on + strandat 2127.663 kb on + strandat 2127.663 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 750 mM
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2,125,556 + gmd b2053 0.30 -0.2
2,125,566 + gmd b2053 0.31 -0.4
2,125,570 + gmd b2053 0.31 -1.0
2,125,570 + gmd b2053 0.31 +0.6
2,125,643 + gmd b2053 0.38 -0.1
2,125,706 + gmd b2053 0.44 -0.4
2,125,707 - gmd b2053 0.44 -2.0
2,125,797 + gmd b2053 0.52 -2.4
2,125,797 + gmd b2053 0.52 +1.6
2,125,828 + gmd b2053 0.54 +0.4
2,125,887 + gmd b2053 0.60 -0.4
2,125,941 - gmd b2053 0.65 -3.4
2,126,048 + gmd b2053 0.74 +0.1
2,126,090 - gmd b2053 0.78 -0.4
2,126,093 - gmd b2053 0.78 -2.0
2,126,151 + gmd b2053 0.83 -1.2
2,126,181 - gmd b2053 0.86 -2.2
2,126,225 - gmd b2053 0.90 -2.0
2,126,225 - gmd b2053 0.90 -2.4
2,126,247 - -1.4
2,126,247 - -2.2
2,126,247 - -2.0
2,126,267 + -0.0
2,126,388 + -1.0
2,126,398 - -2.0
2,126,451 - -0.4
2,126,451 - wcaF b2054 0.16 -3.0
2,126,456 - wcaF b2054 0.17 -1.4
2,126,456 - wcaF b2054 0.17 -0.4
2,126,498 + wcaF b2054 0.24 -0.8
2,126,515 + wcaF b2054 0.28 +0.0
2,126,550 - wcaF b2054 0.34 -3.2
2,126,550 - wcaF b2054 0.34 -2.7
2,126,562 + wcaF b2054 0.36 -0.4
2,126,562 - wcaF b2054 0.36 -3.2
2,126,562 - wcaF b2054 0.36 -2.0
2,126,577 + wcaF b2054 0.39 -1.0
2,126,577 + wcaF b2054 0.39 -0.4
2,126,584 - wcaF b2054 0.40 -2.0
2,126,587 + wcaF b2054 0.41 -1.4
2,126,614 + wcaF b2054 0.46 -1.4
2,126,616 + wcaF b2054 0.46 +0.2
2,126,621 + wcaF b2054 0.47 +0.4
2,126,629 + wcaF b2054 0.48 -0.4
2,126,660 - wcaF b2054 0.54 -3.2
2,126,681 + wcaF b2054 0.58 -1.0
2,126,681 + wcaF b2054 0.58 -2.0
2,126,914 + +1.6
2,127,243 - wcaE b2055 0.42 -4.1
2,127,467 - wcaE b2055 0.72 -3.0
2,127,583 + wcaE b2055 0.88 -0.4
2,127,584 + wcaE b2055 0.88 -1.5
2,127,617 + -1.7
2,127,653 + -1.0
2,127,663 + -3.0
2,127,663 + -2.5

Or see this region's nucleotide sequence