Experiment: m.b. Dimethyl Sulfoxide 8 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0315 and Echvi_0316 overlap by 4 nucleotides Echvi_0316 and Echvi_0317 are separated by 2 nucleotides Echvi_0317 and Echvi_0318 overlap by 4 nucleotides Echvi_0318 and Echvi_0319 are separated by 455 nucleotides
Echvi_0315: Echvi_0315 - Cell division protein FtsI/penicillin-binding protein 2, at 328,155 to 330,266
_0315
Echvi_0316: Echvi_0316 - hypothetical protein, at 330,263 to 330,664
_0316
Echvi_0317: Echvi_0317 - S-adenosyl-methyltransferase MraW, at 330,667 to 331,575
_0317
Echvi_0318: Echvi_0318 - mraZ protein, at 331,572 to 332,024
_0318
Echvi_0319: Echvi_0319 - Geranylgeranyl pyrophosphate synthase, at 332,480 to 333,454
_0319
Position (kb) 330
331
332 Strain fitness (log2 ratio) -2
-1
0
1 at 330.276 kb on - strand at 330.276 kb on - strand at 330.671 kb on - strand at 331.644 kb on - strand, within Echvi_0318 at 331.649 kb on - strand, within Echvi_0318 at 331.693 kb on - strand, within Echvi_0318 at 331.758 kb on - strand, within Echvi_0318 at 331.771 kb on - strand, within Echvi_0318 at 331.803 kb on - strand, within Echvi_0318 at 331.848 kb on - strand, within Echvi_0318 at 332.003 kb on - strand at 332.003 kb on - strand at 332.028 kb on - strand at 332.052 kb on - strand at 332.165 kb on + strand at 332.278 kb on + strand at 332.328 kb on + strand at 332.423 kb on + strand at 332.460 kb on - strand at 332.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 8 vol% remove 330,276 - +0.4 330,276 - +0.1 330,671 - +0.8 331,644 - Echvi_0318 0.16 -1.2 331,649 - Echvi_0318 0.17 -1.0 331,693 - Echvi_0318 0.27 -1.9 331,758 - Echvi_0318 0.41 -1.6 331,771 - Echvi_0318 0.44 -0.4 331,803 - Echvi_0318 0.51 -1.0 331,848 - Echvi_0318 0.61 +0.6 332,003 - -1.3 332,003 - -0.6 332,028 - +0.1 332,052 - -2.0 332,165 + +1.7 332,278 + -0.7 332,328 + -1.2 332,423 + -0.3 332,460 - +0.3 332,465 - -0.0
Or see this region's nucleotide sequence