Experiment: Chlorite 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and mutL are separated by 9 nucleotides mutL and miaA overlap by 8 nucleotides
b4169: amiB - N-acetylmuramoyl-l-alanine amidase II (NCBI), at 4,394,088 to 4,395,425
amiB
b4170: mutL - DNA mismatch repair protein (NCBI), at 4,395,435 to 4,397,282
mutL
b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225
miaA
Position (kb)
4395
4396
4397
4398 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4394.454 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.642 kb on + strand, within amiB at 4394.650 kb on - strand, within amiB at 4394.755 kb on + strand, within amiB at 4394.798 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.850 kb on + strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.912 kb on + strand, within amiB at 4394.917 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.942 kb on + strand, within amiB at 4394.943 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.951 kb on - strand, within amiB at 4394.951 kb on - strand, within amiB at 4395.041 kb on + strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.116 kb on - strand, within amiB at 4395.214 kb on + strand, within amiB at 4395.217 kb on + strand, within amiB at 4395.246 kb on + strand, within amiB at 4395.286 kb on + strand, within amiB at 4395.307 kb on - strand at 4395.307 kb on - strand at 4395.348 kb on + strand at 4395.348 kb on + strand at 4395.414 kb on - strand at 4395.454 kb on + strand at 4395.596 kb on - strand at 4395.655 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.775 kb on + strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.853 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.861 kb on - strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.986 kb on - strand, within mutL at 4395.986 kb on - strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.079 kb on + strand, within mutL at 4396.148 kb on - strand, within mutL at 4396.248 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.326 kb on - strand, within mutL at 4396.329 kb on - strand, within mutL at 4396.407 kb on + strand, within mutL at 4396.415 kb on + strand, within mutL at 4396.415 kb on - strand, within mutL at 4396.432 kb on - strand, within mutL at 4396.435 kb on - strand, within mutL at 4396.435 kb on - strand, within mutL at 4396.466 kb on - strand, within mutL at 4396.546 kb on + strand, within mutL at 4396.551 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.778 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4397.012 kb on + strand, within mutL at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.170 kb on - strand at 4397.195 kb on - strand at 4397.195 kb on - strand at 4397.244 kb on + strand at 4397.244 kb on + strand at 4397.420 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.455 kb on + strand, within miaA at 4397.458 kb on - strand, within miaA at 4397.505 kb on + strand, within miaA at 4397.549 kb on - strand, within miaA at 4397.584 kb on + strand, within miaA at 4397.589 kb on - strand, within miaA at 4397.704 kb on - strand, within miaA at 4397.763 kb on - strand, within miaA at 4397.845 kb on - strand, within miaA at 4397.853 kb on + strand, within miaA at 4397.856 kb on + strand, within miaA at 4397.866 kb on + strand, within miaA at 4397.922 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4398.137 kb on + strand at 4398.137 kb on + strand at 4398.156 kb on + strand at 4398.233 kb on + strand at 4398.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1.25 mM remove 4,394,454 + amiB b4169 0.27 +0.6 4,394,553 + amiB b4169 0.35 +1.2 4,394,553 + amiB b4169 0.35 -0.0 4,394,603 - amiB b4169 0.38 -1.2 4,394,603 - amiB b4169 0.38 +2.9 4,394,606 - amiB b4169 0.39 -0.2 4,394,606 - amiB b4169 0.39 -0.7 4,394,642 + amiB b4169 0.41 +3.0 4,394,650 - amiB b4169 0.42 +0.6 4,394,755 + amiB b4169 0.50 +0.2 4,394,798 - amiB b4169 0.53 +0.9 4,394,832 - amiB b4169 0.56 +2.2 4,394,832 - amiB b4169 0.56 -0.2 4,394,850 + amiB b4169 0.57 +1.3 4,394,856 - amiB b4169 0.57 +3.2 4,394,856 - amiB b4169 0.57 +1.3 4,394,912 + amiB b4169 0.62 +3.2 4,394,917 - amiB b4169 0.62 -0.7 4,394,939 - amiB b4169 0.64 -0.0 4,394,939 - amiB b4169 0.64 -1.4 4,394,942 + amiB b4169 0.64 +0.6 4,394,943 + amiB b4169 0.64 +1.5 4,394,944 + amiB b4169 0.64 -0.2 4,394,944 + amiB b4169 0.64 -0.4 4,394,951 - amiB b4169 0.64 +0.6 4,394,951 - amiB b4169 0.64 -0.4 4,395,041 + amiB b4169 0.71 +1.0 4,395,080 - amiB b4169 0.74 -0.4 4,395,080 - amiB b4169 0.74 +1.6 4,395,112 + amiB b4169 0.77 -0.7 4,395,112 + amiB b4169 0.77 +0.6 4,395,116 - amiB b4169 0.77 -1.0 4,395,214 + amiB b4169 0.84 +1.3 4,395,217 + amiB b4169 0.84 +0.7 4,395,246 + amiB b4169 0.87 +0.9 4,395,286 + amiB b4169 0.90 +0.8 4,395,307 - -2.2 4,395,307 - -0.4 4,395,348 + -0.4 4,395,348 + +1.6 4,395,414 - +0.1 4,395,454 + +1.0 4,395,596 - +0.3 4,395,655 + mutL b4170 0.12 -0.2 4,395,679 + mutL b4170 0.13 +1.6 4,395,679 + mutL b4170 0.13 -1.0 4,395,775 + mutL b4170 0.18 -3.0 4,395,808 - mutL b4170 0.20 -0.6 4,395,808 - mutL b4170 0.20 -0.2 4,395,808 - mutL b4170 0.20 +1.6 4,395,853 + mutL b4170 0.23 -0.0 4,395,854 + mutL b4170 0.23 -1.0 4,395,854 + mutL b4170 0.23 +0.6 4,395,861 - mutL b4170 0.23 -0.4 4,395,865 + mutL b4170 0.23 +0.8 4,395,865 + mutL b4170 0.23 +0.1 4,395,872 - mutL b4170 0.24 +1.6 4,395,872 - mutL b4170 0.24 +0.6 4,395,873 - mutL b4170 0.24 +1.2 4,395,873 - mutL b4170 0.24 +1.3 4,395,920 + mutL b4170 0.26 +1.0 4,395,920 + mutL b4170 0.26 +0.6 4,395,986 - mutL b4170 0.30 -1.0 4,395,986 - mutL b4170 0.30 -0.6 4,396,002 + mutL b4170 0.31 +1.6 4,396,002 + mutL b4170 0.31 -0.1 4,396,079 + mutL b4170 0.35 +0.6 4,396,148 - mutL b4170 0.39 -0.6 4,396,248 + mutL b4170 0.44 +0.8 4,396,271 + mutL b4170 0.45 +1.2 4,396,271 + mutL b4170 0.45 +1.5 4,396,326 - mutL b4170 0.48 +1.2 4,396,329 - mutL b4170 0.48 -1.7 4,396,407 + mutL b4170 0.53 -1.2 4,396,415 + mutL b4170 0.53 +2.2 4,396,415 - mutL b4170 0.53 -0.3 4,396,432 - mutL b4170 0.54 +0.2 4,396,435 - mutL b4170 0.54 -0.4 4,396,435 - mutL b4170 0.54 -2.6 4,396,466 - mutL b4170 0.56 -2.9 4,396,546 + mutL b4170 0.60 +0.8 4,396,551 + mutL b4170 0.60 +1.3 4,396,556 + mutL b4170 0.61 +2.2 4,396,556 + mutL b4170 0.61 +0.6 4,396,556 + mutL b4170 0.61 +1.9 4,396,778 + mutL b4170 0.73 +2.6 4,396,991 + mutL b4170 0.84 +1.8 4,396,991 + mutL b4170 0.84 +2.0 4,396,991 + mutL b4170 0.84 +1.5 4,396,999 - mutL b4170 0.85 -1.0 4,396,999 - mutL b4170 0.85 +0.6 4,396,999 - mutL b4170 0.85 -0.7 4,396,999 - mutL b4170 0.85 +0.6 4,397,012 + mutL b4170 0.85 +1.5 4,397,162 + +2.2 4,397,162 + +2.5 4,397,162 + +2.5 4,397,162 + +1.9 4,397,170 - -2.9 4,397,195 - +0.5 4,397,195 - +1.4 4,397,244 + +1.9 4,397,244 + +3.6 4,397,420 + miaA b4171 0.15 -0.0 4,397,449 + miaA b4171 0.18 -0.4 4,397,449 + miaA b4171 0.18 +0.6 4,397,455 + miaA b4171 0.19 +2.0 4,397,458 - miaA b4171 0.19 -2.7 4,397,505 + miaA b4171 0.24 -2.9 4,397,549 - miaA b4171 0.29 +1.6 4,397,584 + miaA b4171 0.32 -0.2 4,397,589 - miaA b4171 0.33 -1.6 4,397,704 - miaA b4171 0.45 -1.7 4,397,763 - miaA b4171 0.51 +0.6 4,397,845 - miaA b4171 0.60 -0.4 4,397,853 + miaA b4171 0.61 -1.4 4,397,856 + miaA b4171 0.61 -1.4 4,397,866 + miaA b4171 0.62 -0.4 4,397,922 + miaA b4171 0.68 -0.7 4,397,935 + miaA b4171 0.69 -2.6 4,397,935 + miaA b4171 0.69 +1.9 4,398,137 + +1.6 4,398,137 + +2.6 4,398,156 + +4.4 4,398,233 + +3.5 4,398,233 + +2.4
Or see this region's nucleotide sequence