Strain Fitness in Escherichia coli BW25113 around b3998

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemE and nfi are separated by 9 nucleotidesnfi and yjaG are separated by 42 nucleotidesyjaG and hupA are separated by 186 nucleotides b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803 hemE b3998: nfi - endonuclease V (deoxyinosine 3'endoduclease) (VIMSS), at 4,196,813 to 4,197,484 nfi b3999: yjaG - hypothetical protein (NCBI), at 4,197,527 to 4,198,117 yjaG b4000: hupA - HU, DNA-binding transcriptional regulator, alpha subunit (NCBI), at 4,198,304 to 4,198,576 hupA Position (kb) 4196 4197 4198Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4196.940 kb on - strand, within nfiat 4196.973 kb on - strand, within nfiat 4197.077 kb on + strand, within nfiat 4197.155 kb on - strand, within nfiat 4197.274 kb on - strand, within nfiat 4197.274 kb on - strand, within nfiat 4197.291 kb on + strand, within nfiat 4197.291 kb on + strand, within nfiat 4197.316 kb on + strand, within nfiat 4197.331 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.353 kb on + strand, within nfiat 4197.361 kb on - strand, within nfiat 4197.361 kb on - strand, within nfiat 4197.383 kb on - strand, within nfiat 4197.383 kb on - strand, within nfiat 4197.436 kb on - strandat 4197.436 kb on - strandat 4197.471 kb on - strandat 4197.593 kb on - strand, within yjaGat 4197.608 kb on + strand, within yjaGat 4197.618 kb on - strand, within yjaGat 4197.618 kb on - strand, within yjaGat 4197.733 kb on - strand, within yjaGat 4197.855 kb on - strand, within yjaGat 4197.855 kb on - strand, within yjaGat 4197.871 kb on + strand, within yjaGat 4197.879 kb on - strand, within yjaGat 4197.911 kb on + strand, within yjaGat 4197.911 kb on + strand, within yjaGat 4197.983 kb on - strand, within yjaGat 4198.028 kb on + strand, within yjaGat 4198.028 kb on + strand, within yjaGat 4198.070 kb on - strandat 4198.162 kb on + strandat 4198.235 kb on - strandat 4198.245 kb on - strandat 4198.250 kb on - strandat 4198.266 kb on + strandat 4198.287 kb on - strandat 4198.287 kb on - strandat 4198.359 kb on - strand, within hupAat 4198.359 kb on - strand, within hupAat 4198.380 kb on + strand, within hupAat 4198.380 kb on + strand, within hupAat 4198.388 kb on - strand, within hupAat 4198.409 kb on + strand, within hupAat 4198.409 kb on + strand, within hupAat 4198.425 kb on + strand, within hupAat 4198.443 kb on - strand, within hupAat 4198.457 kb on - strand, within hupA

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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4,196,940 - nfi b3998 0.19 -3.4
4,196,973 - nfi b3998 0.24 +0.2
4,197,077 + nfi b3998 0.39 -0.6
4,197,155 - nfi b3998 0.51 -0.4
4,197,274 - nfi b3998 0.69 -0.8
4,197,274 - nfi b3998 0.69 -2.0
4,197,291 + nfi b3998 0.71 -0.4
4,197,291 + nfi b3998 0.71 -1.0
4,197,316 + nfi b3998 0.75 -2.3
4,197,331 + nfi b3998 0.77 +0.7
4,197,353 + nfi b3998 0.80 -1.8
4,197,353 + nfi b3998 0.80 -0.3
4,197,353 + nfi b3998 0.80 +0.7
4,197,361 - nfi b3998 0.82 -1.7
4,197,361 - nfi b3998 0.82 -0.4
4,197,383 - nfi b3998 0.85 -1.5
4,197,383 - nfi b3998 0.85 -1.4
4,197,436 - +0.0
4,197,436 - -1.4
4,197,471 - +0.4
4,197,593 - yjaG b3999 0.11 +0.1
4,197,608 + yjaG b3999 0.14 +0.4
4,197,618 - yjaG b3999 0.15 +1.2
4,197,618 - yjaG b3999 0.15 +0.6
4,197,733 - yjaG b3999 0.35 -0.8
4,197,855 - yjaG b3999 0.55 -0.4
4,197,855 - yjaG b3999 0.55 +0.0
4,197,871 + yjaG b3999 0.58 -1.4
4,197,879 - yjaG b3999 0.60 +0.3
4,197,911 + yjaG b3999 0.65 +2.1
4,197,911 + yjaG b3999 0.65 -0.2
4,197,983 - yjaG b3999 0.77 -1.4
4,198,028 + yjaG b3999 0.85 -0.4
4,198,028 + yjaG b3999 0.85 +1.6
4,198,070 - -0.1
4,198,162 + +1.9
4,198,235 - +0.0
4,198,245 - +0.4
4,198,250 - +0.3
4,198,266 + +0.5
4,198,287 - +2.6
4,198,287 - +1.4
4,198,359 - hupA b4000 0.20 +0.3
4,198,359 - hupA b4000 0.20 +2.6
4,198,380 + hupA b4000 0.28 -0.4
4,198,380 + hupA b4000 0.28 +1.3
4,198,388 - hupA b4000 0.31 -0.4
4,198,409 + hupA b4000 0.38 +4.2
4,198,409 + hupA b4000 0.38 +0.8
4,198,425 + hupA b4000 0.44 -0.9
4,198,443 - hupA b4000 0.51 +1.6
4,198,457 - hupA b4000 0.56 +1.0

Or see this region's nucleotide sequence