Experiment: Chlorite 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt priA and rpmE are separated by 202 nucleotides rpmE and yiiX are separated by 60 nucleotides yiiX and metJ are separated by 183 nucleotides metJ and metB are separated by 276 nucleotides
b3935: priA - primosome assembly protein PriA (NCBI), at 4,122,635 to 4,124,833
priA
b3936: rpmE - 50S ribosomal subunit protein L31 (NCBI), at 4,125,036 to 4,125,248
rpmE
b3937: yiiX - predicted peptidoglycan peptidase (NCBI), at 4,125,309 to 4,125,917
yiiX
b3938: metJ - transcriptional repressor protein MetJ (NCBI), at 4,126,101 to 4,126,418
metJ
b3939: metB - cystathionine gamma-synthase (NCBI), at 4,126,695 to 4,127,855
metB
Position (kb) 4125
4126 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4124.669 kb on - strand at 4124.791 kb on - strand at 4124.791 kb on - strand at 4124.835 kb on + strand at 4124.839 kb on + strand at 4124.865 kb on + strand at 4124.878 kb on + strand at 4124.886 kb on - strand at 4124.896 kb on - strand at 4124.959 kb on + strand at 4125.289 kb on - strand at 4125.289 kb on - strand at 4125.381 kb on - strand, within yiiX at 4125.381 kb on - strand, within yiiX at 4125.485 kb on - strand, within yiiX at 4125.494 kb on + strand, within yiiX at 4125.515 kb on + strand, within yiiX at 4125.527 kb on + strand, within yiiX at 4125.527 kb on + strand, within yiiX at 4125.527 kb on + strand, within yiiX at 4125.527 kb on + strand, within yiiX at 4125.537 kb on + strand, within yiiX at 4125.537 kb on + strand, within yiiX at 4125.545 kb on - strand, within yiiX at 4125.545 kb on - strand, within yiiX at 4125.631 kb on - strand, within yiiX at 4125.647 kb on - strand, within yiiX at 4125.661 kb on + strand, within yiiX at 4125.685 kb on + strand, within yiiX at 4125.690 kb on + strand, within yiiX at 4125.713 kb on - strand, within yiiX at 4125.713 kb on - strand, within yiiX at 4125.758 kb on + strand, within yiiX at 4125.768 kb on - strand, within yiiX at 4125.784 kb on + strand, within yiiX at 4125.791 kb on - strand, within yiiX at 4125.792 kb on - strand, within yiiX at 4125.792 kb on - strand, within yiiX at 4125.903 kb on - strand at 4125.948 kb on + strand at 4125.969 kb on - strand at 4125.969 kb on - strand at 4126.055 kb on + strand at 4126.073 kb on + strand at 4126.073 kb on + strand at 4126.073 kb on + strand at 4126.073 kb on + strand at 4126.081 kb on - strand at 4126.123 kb on - strand at 4126.123 kb on - strand at 4126.272 kb on - strand at 4126.323 kb on + strand, within metJ at 4126.373 kb on - strand, within metJ at 4126.373 kb on - strand, within metJ at 4126.389 kb on - strand at 4126.414 kb on + strand at 4126.414 kb on + strand at 4126.415 kb on - strand at 4126.422 kb on - strand at 4126.524 kb on - strand at 4126.524 kb on - strand at 4126.526 kb on + strand at 4126.644 kb on - strand at 4126.648 kb on - strand at 4126.707 kb on - strand at 4126.717 kb on - strand at 4126.717 kb on - strand at 4126.719 kb on + strand at 4126.722 kb on + strand at 4126.759 kb on - strand at 4126.900 kb on + strand, within metB at 4126.900 kb on + strand, within metB at 4126.902 kb on - strand, within metB at 4126.908 kb on - strand, within metB at 4126.910 kb on + strand, within metB at 4126.913 kb on - strand, within metB
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1.25 mM remove 4,124,669 - +1.0 4,124,791 - -2.0 4,124,791 - +0.0 4,124,835 + +0.4 4,124,839 + +0.5 4,124,865 + -0.6 4,124,878 + +0.2 4,124,886 - +0.3 4,124,896 - -0.6 4,124,959 + +0.3 4,125,289 - -1.7 4,125,289 - +1.0 4,125,381 - yiiX b3937 0.12 +0.2 4,125,381 - yiiX b3937 0.12 +0.6 4,125,485 - yiiX b3937 0.29 +0.6 4,125,494 + yiiX b3937 0.30 +1.0 4,125,515 + yiiX b3937 0.34 +2.0 4,125,527 + yiiX b3937 0.36 +1.0 4,125,527 + yiiX b3937 0.36 -0.1 4,125,527 + yiiX b3937 0.36 +0.0 4,125,527 + yiiX b3937 0.36 +0.4 4,125,537 + yiiX b3937 0.37 +0.6 4,125,537 + yiiX b3937 0.37 +0.5 4,125,545 - yiiX b3937 0.39 -1.1 4,125,545 - yiiX b3937 0.39 +0.2 4,125,631 - yiiX b3937 0.53 +0.2 4,125,647 - yiiX b3937 0.56 +1.5 4,125,661 + yiiX b3937 0.58 +2.0 4,125,685 + yiiX b3937 0.62 +0.0 4,125,690 + yiiX b3937 0.63 +1.4 4,125,713 - yiiX b3937 0.66 +1.2 4,125,713 - yiiX b3937 0.66 +1.6 4,125,758 + yiiX b3937 0.74 +1.6 4,125,768 - yiiX b3937 0.75 -0.4 4,125,784 + yiiX b3937 0.78 +0.9 4,125,791 - yiiX b3937 0.79 +1.0 4,125,792 - yiiX b3937 0.79 -0.4 4,125,792 - yiiX b3937 0.79 -0.4 4,125,903 - +1.5 4,125,948 + -0.2 4,125,969 - +0.6 4,125,969 - +0.2 4,126,055 + +0.1 4,126,073 + -0.7 4,126,073 + +0.3 4,126,073 + +0.8 4,126,073 + +0.2 4,126,081 - -0.1 4,126,123 - -0.4 4,126,123 - -0.4 4,126,272 - +1.6 4,126,323 + metJ b3938 0.70 +2.0 4,126,373 - metJ b3938 0.86 +0.6 4,126,373 - metJ b3938 0.86 +3.0 4,126,389 - -0.4 4,126,414 + +1.3 4,126,414 + +1.2 4,126,415 - -0.6 4,126,422 - -1.4 4,126,524 - -0.4 4,126,524 - +0.6 4,126,526 + -0.8 4,126,644 - +0.6 4,126,648 - +0.2 4,126,707 - -0.1 4,126,717 - +0.6 4,126,717 - +0.6 4,126,719 + -1.0 4,126,722 + +0.4 4,126,759 - -0.4 4,126,900 + metB b3939 0.18 +1.1 4,126,900 + metB b3939 0.18 -0.4 4,126,902 - metB b3939 0.18 +1.2 4,126,908 - metB b3939 0.18 +0.6 4,126,910 + metB b3939 0.19 +1.6 4,126,913 - metB b3939 0.19 +0.4
Or see this region's nucleotide sequence