Strain Fitness in Escherichia coli BW25113 around b2395

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyi81_3 and yfeA are separated by 199 nucleotidesyfeA and alaX are separated by 208 nucleotidesalaX and alaW are separated by 39 nucleotidesalaW and yfeC are separated by 235 nucleotidesyfeC and yfeD are separated by 1 nucleotides b2394: yi81_3 - IS186 hypothetical protein (VIMSS), at 2,512,353 to 2,513,465 _3 b2395: yfeA - orf, hypothetical protein (VIMSS), at 2,513,665 to 2,515,854 yfeA b2396: alaX - tRNA-Ala (NCBI), at 2,516,063 to 2,516,138 alaX b2397: alaW - tRNA-Ala (NCBI), at 2,516,178 to 2,516,253 alaW b2398: yfeC - orf, hypothetical protein (VIMSS), at 2,516,489 to 2,516,833 yfeC b2399: yfeD - predicted DNA-binding transcriptional regulator (RefSeq), at 2,516,835 to 2,517,227 yfeD Position (kb) 2513 2514 2515 2516Strain fitness (log2 ratio) -3 -2 -1 0 1at 2513.680 kb on - strandat 2513.680 kb on - strandat 2513.751 kb on - strandat 2513.769 kb on + strandat 2513.775 kb on + strandat 2514.032 kb on - strand, within yfeAat 2514.112 kb on - strand, within yfeAat 2514.161 kb on + strand, within yfeAat 2514.223 kb on + strand, within yfeAat 2514.223 kb on - strand, within yfeAat 2514.235 kb on - strand, within yfeAat 2514.345 kb on - strand, within yfeAat 2514.345 kb on - strand, within yfeAat 2514.354 kb on + strand, within yfeAat 2514.355 kb on + strand, within yfeAat 2514.369 kb on + strand, within yfeAat 2514.396 kb on - strand, within yfeAat 2514.413 kb on + strand, within yfeAat 2514.413 kb on + strand, within yfeAat 2514.425 kb on + strand, within yfeAat 2514.428 kb on - strand, within yfeAat 2514.436 kb on + strand, within yfeAat 2514.458 kb on - strand, within yfeAat 2514.458 kb on - strand, within yfeAat 2514.493 kb on - strand, within yfeAat 2514.529 kb on + strand, within yfeAat 2514.547 kb on - strand, within yfeAat 2514.547 kb on - strand, within yfeAat 2514.563 kb on + strand, within yfeAat 2514.563 kb on + strand, within yfeAat 2514.752 kb on - strand, within yfeAat 2514.860 kb on - strand, within yfeAat 2514.872 kb on + strand, within yfeAat 2514.928 kb on + strand, within yfeAat 2514.930 kb on + strand, within yfeAat 2514.938 kb on - strand, within yfeAat 2514.966 kb on - strand, within yfeAat 2514.966 kb on - strand, within yfeAat 2515.150 kb on + strand, within yfeAat 2515.165 kb on - strand, within yfeAat 2515.165 kb on - strand, within yfeAat 2515.199 kb on - strand, within yfeAat 2515.236 kb on - strand, within yfeAat 2515.301 kb on - strand, within yfeAat 2515.561 kb on + strand, within yfeAat 2515.744 kb on + strandat 2515.857 kb on + strandat 2515.859 kb on + strandat 2515.859 kb on + strandat 2515.867 kb on - strandat 2515.977 kb on - strandat 2515.986 kb on - strandat 2516.197 kb on - strand, within alaWat 2516.434 kb on - strandat 2516.439 kb on + strandat 2516.439 kb on + strandat 2516.560 kb on - strand, within yfeCat 2516.560 kb on - strand, within yfeCat 2516.568 kb on - strand, within yfeCat 2516.620 kb on - strand, within yfeCat 2516.787 kb on + strand, within yfeCat 2516.808 kb on + strandat 2516.808 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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2,513,680 - +0.9
2,513,680 - -1.5
2,513,751 - +0.1
2,513,769 + -0.9
2,513,775 + -0.9
2,514,032 - yfeA b2395 0.17 -0.9
2,514,112 - yfeA b2395 0.20 -1.6
2,514,161 + yfeA b2395 0.23 +0.1
2,514,223 + yfeA b2395 0.25 +1.5
2,514,223 - yfeA b2395 0.25 +0.6
2,514,235 - yfeA b2395 0.26 +0.5
2,514,345 - yfeA b2395 0.31 +1.1
2,514,345 - yfeA b2395 0.31 +0.5
2,514,354 + yfeA b2395 0.31 -0.3
2,514,355 + yfeA b2395 0.32 -1.1
2,514,369 + yfeA b2395 0.32 -0.9
2,514,396 - yfeA b2395 0.33 -0.5
2,514,413 + yfeA b2395 0.34 -0.5
2,514,413 + yfeA b2395 0.34 +0.7
2,514,425 + yfeA b2395 0.35 -0.3
2,514,428 - yfeA b2395 0.35 -0.9
2,514,436 + yfeA b2395 0.35 -0.5
2,514,458 - yfeA b2395 0.36 -0.4
2,514,458 - yfeA b2395 0.36 +0.8
2,514,493 - yfeA b2395 0.38 -0.9
2,514,529 + yfeA b2395 0.39 -0.4
2,514,547 - yfeA b2395 0.40 -0.4
2,514,547 - yfeA b2395 0.40 -1.8
2,514,563 + yfeA b2395 0.41 -0.2
2,514,563 + yfeA b2395 0.41 -2.9
2,514,752 - yfeA b2395 0.50 -0.5
2,514,860 - yfeA b2395 0.55 -0.2
2,514,872 + yfeA b2395 0.55 -0.9
2,514,928 + yfeA b2395 0.58 -0.3
2,514,930 + yfeA b2395 0.58 +1.4
2,514,938 - yfeA b2395 0.58 -1.1
2,514,966 - yfeA b2395 0.59 -1.9
2,514,966 - yfeA b2395 0.59 -2.5
2,515,150 + yfeA b2395 0.68 -2.2
2,515,165 - yfeA b2395 0.68 +0.7
2,515,165 - yfeA b2395 0.68 -0.6
2,515,199 - yfeA b2395 0.70 -0.2
2,515,236 - yfeA b2395 0.72 +0.4
2,515,301 - yfeA b2395 0.75 -0.2
2,515,561 + yfeA b2395 0.87 -0.9
2,515,744 + -0.9
2,515,857 + -1.9
2,515,859 + +0.1
2,515,859 + -1.1
2,515,867 - +1.1
2,515,977 - -0.5
2,515,986 - -1.3
2,516,197 - alaW b2397 0.25 +0.1
2,516,434 - -1.1
2,516,439 + -0.2
2,516,439 + +0.7
2,516,560 - yfeC b2398 0.21 -1.5
2,516,560 - yfeC b2398 0.21 +0.7
2,516,568 - yfeC b2398 0.23 -0.2
2,516,620 - yfeC b2398 0.38 +0.8
2,516,787 + yfeC b2398 0.86 -0.6
2,516,808 + -0.3
2,516,808 + -2.5

Or see this region's nucleotide sequence