Strain Fitness in Escherichia coli BW25113 around b2105

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiM and yohL are separated by 222 nucleotidesyohL and yohM are separated by 120 nucleotides b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323 thiM b2105: yohL - hypothetical protein (NCBI), at 2,183,546 to 2,183,818 yohL b2106: yohM - membrane protein conferring nickel and cobalt resistance (NCBI), at 2,183,939 to 2,184,763 yohM Position (kb) 2183 2184Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2182.546 kb on - strandat 2182.646 kb on + strand, within thiMat 2183.006 kb on - strand, within thiMat 2183.006 kb on - strand, within thiMat 2183.044 kb on + strand, within thiMat 2183.246 kb on + strandat 2183.246 kb on + strandat 2183.259 kb on - strandat 2183.259 kb on - strandat 2183.389 kb on - strandat 2183.399 kb on - strandat 2183.399 kb on - strandat 2183.399 kb on - strandat 2183.495 kb on - strandat 2183.533 kb on - strandat 2183.614 kb on + strand, within yohLat 2183.614 kb on - strand, within yohLat 2183.620 kb on - strand, within yohLat 2183.621 kb on + strand, within yohLat 2183.766 kb on - strand, within yohLat 2183.814 kb on - strandat 2183.814 kb on - strandat 2183.989 kb on + strandat 2184.011 kb on + strandat 2184.014 kb on + strandat 2184.014 kb on + strandat 2184.030 kb on - strand, within yohMat 2184.141 kb on + strand, within yohMat 2184.141 kb on + strand, within yohMat 2184.272 kb on + strand, within yohMat 2184.349 kb on - strand, within yohMat 2184.356 kb on - strand, within yohMat 2184.356 kb on - strand, within yohMat 2184.382 kb on + strand, within yohMat 2184.493 kb on + strand, within yohMat 2184.493 kb on + strand, within yohMat 2184.501 kb on - strand, within yohMat 2184.509 kb on + strand, within yohMat 2184.541 kb on - strand, within yohMat 2184.543 kb on + strand, within yohMat 2184.551 kb on - strand, within yohMat 2184.556 kb on - strand, within yohMat 2184.579 kb on + strand, within yohMat 2184.606 kb on + strand, within yohMat 2184.606 kb on + strand, within yohMat 2184.630 kb on + strand, within yohMat 2184.671 kb on + strand, within yohMat 2184.681 kb on - strandat 2184.681 kb on - strandat 2184.703 kb on + strandat 2184.710 kb on + strandat 2184.711 kb on + strandat 2184.718 kb on - strandat 2184.722 kb on + strandat 2184.722 kb on + strandat 2184.724 kb on + strandat 2184.724 kb on - strandat 2184.728 kb on - strandat 2184.732 kb on - strandat 2184.748 kb on - strandat 2184.749 kb on + strandat 2184.757 kb on - strandat 2184.757 kb on - strandat 2184.758 kb on - strandat 2184.771 kb on + strandat 2184.771 kb on + strandat 2184.785 kb on + strandat 2184.785 kb on + strandat 2184.788 kb on + strandat 2184.788 kb on + strandat 2184.790 kb on - strandat 2184.790 kb on - strandat 2184.791 kb on - strandat 2184.791 kb on - strandat 2184.793 kb on - strandat 2184.795 kb on + strandat 2184.796 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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2,182,546 - -1.0
2,182,646 + thiM b2104 0.14 +2.4
2,183,006 - thiM b2104 0.60 -0.2
2,183,006 - thiM b2104 0.60 +0.0
2,183,044 + thiM b2104 0.65 -1.0
2,183,246 + -0.3
2,183,246 + +0.2
2,183,259 - -2.0
2,183,259 - +0.5
2,183,389 - +0.0
2,183,399 - -2.6
2,183,399 - -1.2
2,183,399 - -2.6
2,183,495 - +1.0
2,183,533 - +0.6
2,183,614 + yohL b2105 0.25 +0.6
2,183,614 - yohL b2105 0.25 -1.4
2,183,620 - yohL b2105 0.27 +0.6
2,183,621 + yohL b2105 0.27 -0.7
2,183,766 - yohL b2105 0.81 -1.0
2,183,814 - +0.0
2,183,814 - +0.0
2,183,989 + -0.7
2,184,011 + +0.3
2,184,014 + +0.4
2,184,014 + +0.2
2,184,030 - yohM b2106 0.11 -0.3
2,184,141 + yohM b2106 0.24 +0.0
2,184,141 + yohM b2106 0.24 +0.0
2,184,272 + yohM b2106 0.40 -1.8
2,184,349 - yohM b2106 0.50 +0.0
2,184,356 - yohM b2106 0.51 +0.3
2,184,356 - yohM b2106 0.51 +1.0
2,184,382 + yohM b2106 0.54 +2.0
2,184,493 + yohM b2106 0.67 +0.0
2,184,493 + yohM b2106 0.67 +0.5
2,184,501 - yohM b2106 0.68 -0.6
2,184,509 + yohM b2106 0.69 +3.3
2,184,541 - yohM b2106 0.73 -2.8
2,184,543 + yohM b2106 0.73 +0.8
2,184,551 - yohM b2106 0.74 +0.8
2,184,556 - yohM b2106 0.75 -3.3
2,184,579 + yohM b2106 0.78 -2.0
2,184,606 + yohM b2106 0.81 -0.7
2,184,606 + yohM b2106 0.81 -0.4
2,184,630 + yohM b2106 0.84 +2.2
2,184,671 + yohM b2106 0.89 -1.2
2,184,681 - -1.0
2,184,681 - -1.0
2,184,703 + -0.2
2,184,710 + -0.5
2,184,711 + -0.7
2,184,718 - +1.0
2,184,722 + +0.4
2,184,722 + +0.8
2,184,724 + +0.0
2,184,724 - -1.6
2,184,728 - +1.4
2,184,732 - +0.6
2,184,748 - -0.4
2,184,749 + +1.2
2,184,757 - +0.8
2,184,757 - +0.7
2,184,758 - -1.0
2,184,771 + +1.6
2,184,771 + -1.1
2,184,785 + +0.4
2,184,785 + -0.2
2,184,788 + -0.3
2,184,788 + +0.1
2,184,790 - +0.0
2,184,790 - +0.4
2,184,791 - +0.0
2,184,791 - -1.0
2,184,793 - +2.3
2,184,795 + -1.6
2,184,796 - -1.2

Or see this region's nucleotide sequence