Strain Fitness in Escherichia coli BW25113 around b2016

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeY and yeeZ are separated by 45 nucleotidesyeeZ and yoeB are separated by 82 nucleotidesyoeB and yefM overlap by 4 nucleotidesyefM and hisL are separated by 282 nucleotides b2015: yeeY - putative transcriptional regulator LYSR-type (VIMSS), at 2,085,353 to 2,086,282 yeeY b2016: yeeZ - predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,086,328 to 2,087,152 yeeZ b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489 yoeB b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737 yefM b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070 hisL Position (kb) 2086 2087 2088Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2085.736 kb on + strand, within yeeYat 2085.786 kb on + strand, within yeeYat 2085.813 kb on + strand, within yeeYat 2085.852 kb on - strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.866 kb on + strand, within yeeYat 2086.064 kb on - strand, within yeeYat 2086.173 kb on + strand, within yeeYat 2086.181 kb on - strand, within yeeYat 2086.181 kb on - strand, within yeeYat 2086.264 kb on - strandat 2086.298 kb on - strandat 2086.338 kb on + strandat 2086.338 kb on + strandat 2086.351 kb on + strandat 2086.424 kb on + strand, within yeeZat 2086.458 kb on - strand, within yeeZat 2086.458 kb on - strand, within yeeZat 2086.500 kb on + strand, within yeeZat 2086.556 kb on - strand, within yeeZat 2086.579 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.669 kb on + strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.871 kb on - strand, within yeeZat 2086.947 kb on - strand, within yeeZat 2087.058 kb on + strand, within yeeZat 2087.122 kb on + strandat 2087.178 kb on - strandat 2087.178 kb on - strandat 2087.187 kb on - strandat 2087.197 kb on - strandat 2087.364 kb on - strand, within yoeBat 2087.506 kb on - strandat 2087.506 kb on - strandat 2087.544 kb on + strand, within yefMat 2087.642 kb on - strand, within yefMat 2087.867 kb on + strandat 2088.113 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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2,085,736 + yeeY b2015 0.41 -1.0
2,085,786 + yeeY b2015 0.47 +1.0
2,085,813 + yeeY b2015 0.49 -0.3
2,085,852 - yeeY b2015 0.54 -1.0
2,085,860 + yeeY b2015 0.55 -0.9
2,085,860 + yeeY b2015 0.55 +0.3
2,085,866 + yeeY b2015 0.55 +0.3
2,086,064 - yeeY b2015 0.76 -0.6
2,086,173 + yeeY b2015 0.88 -1.6
2,086,181 - yeeY b2015 0.89 -0.8
2,086,181 - yeeY b2015 0.89 +0.7
2,086,264 - +2.0
2,086,298 - +0.7
2,086,338 + +0.2
2,086,338 + -2.4
2,086,351 + -2.2
2,086,424 + yeeZ b2016 0.12 -3.2
2,086,458 - yeeZ b2016 0.16 -3.0
2,086,458 - yeeZ b2016 0.16 -1.4
2,086,500 + yeeZ b2016 0.21 -2.0
2,086,556 - yeeZ b2016 0.28 -0.6
2,086,579 + yeeZ b2016 0.30 -3.5
2,086,660 + yeeZ b2016 0.40 -2.4
2,086,660 + yeeZ b2016 0.40 -2.0
2,086,669 + yeeZ b2016 0.41 -0.0
2,086,672 - yeeZ b2016 0.42 -1.0
2,086,672 - yeeZ b2016 0.42 -2.0
2,086,871 - yeeZ b2016 0.66 -3.2
2,086,947 - yeeZ b2016 0.75 -3.5
2,087,058 + yeeZ b2016 0.88 -3.3
2,087,122 + -2.4
2,087,178 - +2.6
2,087,178 - -0.0
2,087,187 - +1.2
2,087,197 - -1.0
2,087,364 - yoeB b4539 0.51 -0.0
2,087,506 - -0.0
2,087,506 - -0.0
2,087,544 + yefM b2017 0.23 -2.4
2,087,642 - yefM b2017 0.62 +0.5
2,087,867 + +0.1
2,088,113 - +1.2

Or see this region's nucleotide sequence