Experiment: Chlorite 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides amyA and yedD are separated by 33 nucleotides yedD and yedE are separated by 186 nucleotides
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737
fliS
b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102
fliT
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114
yedD
b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506
yedE
Position (kb)
2004
2005
2006 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2003.414 kb on + strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.462 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.699 kb on + strand at 2003.734 kb on + strand at 2003.735 kb on - strand at 2003.738 kb on - strand at 2003.923 kb on - strand, within fliT at 2003.933 kb on - strand, within fliT at 2004.035 kb on + strand, within fliT at 2004.073 kb on + strand at 2004.135 kb on + strand at 2004.148 kb on + strand at 2004.241 kb on + strand at 2004.241 kb on + strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.299 kb on + strand at 2004.446 kb on + strand, within amyA at 2004.461 kb on - strand, within amyA at 2004.533 kb on + strand, within amyA at 2004.543 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.739 kb on - strand, within amyA at 2004.809 kb on + strand, within amyA at 2004.861 kb on - strand, within amyA at 2004.942 kb on - strand, within amyA at 2005.017 kb on - strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.185 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.310 kb on + strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.443 kb on - strand, within amyA at 2005.591 kb on - strand at 2005.633 kb on + strand at 2005.633 kb on + strand at 2005.659 kb on - strand at 2005.659 kb on - strand at 2005.729 kb on + strand at 2005.787 kb on - strand, within yedD at 2005.788 kb on + strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.809 kb on - strand, within yedD at 2005.827 kb on - strand, within yedD at 2005.953 kb on + strand, within yedD at 2005.969 kb on - strand, within yedD at 2006.256 kb on - strand at 2006.284 kb on + strand at 2006.393 kb on - strand at 2006.393 kb on - strand at 2006.417 kb on + strand at 2006.417 kb on + strand at 2006.457 kb on - strand, within yedE at 2006.508 kb on + strand, within yedE at 2006.605 kb on - strand, within yedE
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1.25 mM remove 2,003,414 + fliS b1925 0.21 +0.6 2,003,451 - fliS b1925 0.30 +1.4 2,003,451 - fliS b1925 0.30 -0.8 2,003,451 - fliS b1925 0.30 -0.3 2,003,462 + fliS b1925 0.33 -0.7 2,003,544 + fliS b1925 0.53 +0.4 2,003,544 + fliS b1925 0.53 -1.4 2,003,699 + +0.9 2,003,734 + -0.5 2,003,735 - -0.1 2,003,738 - -0.3 2,003,923 - fliT b1926 0.51 -2.4 2,003,933 - fliT b1926 0.54 +2.3 2,004,035 + fliT b1926 0.81 +0.6 2,004,073 + -1.1 2,004,135 + -2.4 2,004,148 + +0.7 2,004,241 + -0.4 2,004,241 + -0.8 2,004,249 - +0.5 2,004,249 - +1.5 2,004,249 - -0.1 2,004,299 + -1.6 2,004,446 + amyA b1927 0.18 -0.8 2,004,461 - amyA b1927 0.19 +0.1 2,004,533 + amyA b1927 0.24 +0.9 2,004,543 - amyA b1927 0.24 -0.1 2,004,614 - amyA b1927 0.29 +2.0 2,004,614 - amyA b1927 0.29 -0.1 2,004,739 - amyA b1927 0.38 -0.7 2,004,809 + amyA b1927 0.42 +0.5 2,004,861 - amyA b1927 0.46 +0.7 2,004,942 - amyA b1927 0.51 -0.5 2,005,017 - amyA b1927 0.56 +0.9 2,005,185 + amyA b1927 0.68 +1.5 2,005,185 + amyA b1927 0.68 +0.9 2,005,308 + amyA b1927 0.76 +0.9 2,005,308 + amyA b1927 0.76 +0.5 2,005,310 + amyA b1927 0.76 +0.9 2,005,410 - amyA b1927 0.83 +1.0 2,005,410 - amyA b1927 0.83 -0.6 2,005,443 - amyA b1927 0.85 +0.5 2,005,591 - -0.8 2,005,633 + -1.4 2,005,633 + +0.9 2,005,659 - +0.9 2,005,659 - -0.1 2,005,729 + +0.6 2,005,787 - yedD b1928 0.21 +0.6 2,005,788 + yedD b1928 0.21 +0.9 2,005,801 - yedD b1928 0.24 +1.8 2,005,801 - yedD b1928 0.24 -1.6 2,005,809 - yedD b1928 0.26 -0.1 2,005,827 - yedD b1928 0.30 -0.1 2,005,953 + yedD b1928 0.61 +0.9 2,005,969 - yedD b1928 0.65 +1.4 2,006,256 - +1.5 2,006,284 + +0.2 2,006,393 - +0.6 2,006,393 - +0.7 2,006,417 + -3.0 2,006,417 + +0.2 2,006,457 - yedE b1929 0.13 +0.7 2,006,508 + yedE b1929 0.17 -0.5 2,006,605 - yedE b1929 0.25 -0.1
Or see this region's nucleotide sequence