Strain Fitness in Escherichia coli BW25113 around b1927

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliD and fliS are separated by 24 nucleotidesfliS and fliT overlap by 1 nucleotidesfliT and amyA are separated by 77 nucleotidesamyA and yedD are separated by 33 nucleotidesyedD and yedE are separated by 186 nucleotides b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302 fliD b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737 fliS b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102 fliT b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667 amyA b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114 yedD b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506 yedE Position (kb) 2004 2005 2006Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2003.414 kb on + strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.451 kb on - strand, within fliSat 2003.462 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.544 kb on + strand, within fliSat 2003.699 kb on + strandat 2003.734 kb on + strandat 2003.735 kb on - strandat 2003.738 kb on - strandat 2003.923 kb on - strand, within fliTat 2003.933 kb on - strand, within fliTat 2004.035 kb on + strand, within fliTat 2004.073 kb on + strandat 2004.135 kb on + strandat 2004.148 kb on + strandat 2004.241 kb on + strandat 2004.241 kb on + strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.249 kb on - strandat 2004.299 kb on + strandat 2004.446 kb on + strand, within amyAat 2004.461 kb on - strand, within amyAat 2004.533 kb on + strand, within amyAat 2004.543 kb on - strand, within amyAat 2004.614 kb on - strand, within amyAat 2004.614 kb on - strand, within amyAat 2004.739 kb on - strand, within amyAat 2004.809 kb on + strand, within amyAat 2004.861 kb on - strand, within amyAat 2004.942 kb on - strand, within amyAat 2005.017 kb on - strand, within amyAat 2005.185 kb on + strand, within amyAat 2005.185 kb on + strand, within amyAat 2005.308 kb on + strand, within amyAat 2005.308 kb on + strand, within amyAat 2005.310 kb on + strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.443 kb on - strand, within amyAat 2005.591 kb on - strandat 2005.633 kb on + strandat 2005.633 kb on + strandat 2005.659 kb on - strandat 2005.659 kb on - strandat 2005.729 kb on + strandat 2005.787 kb on - strand, within yedDat 2005.788 kb on + strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.809 kb on - strand, within yedDat 2005.827 kb on - strand, within yedDat 2005.953 kb on + strand, within yedDat 2005.969 kb on - strand, within yedDat 2006.256 kb on - strandat 2006.284 kb on + strandat 2006.393 kb on - strandat 2006.393 kb on - strandat 2006.417 kb on + strandat 2006.417 kb on + strandat 2006.457 kb on - strand, within yedEat 2006.508 kb on + strand, within yedEat 2006.605 kb on - strand, within yedE

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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2,003,414 + fliS b1925 0.21 +0.6
2,003,451 - fliS b1925 0.30 +1.4
2,003,451 - fliS b1925 0.30 -0.8
2,003,451 - fliS b1925 0.30 -0.3
2,003,462 + fliS b1925 0.33 -0.7
2,003,544 + fliS b1925 0.53 +0.4
2,003,544 + fliS b1925 0.53 -1.4
2,003,699 + +0.9
2,003,734 + -0.5
2,003,735 - -0.1
2,003,738 - -0.3
2,003,923 - fliT b1926 0.51 -2.4
2,003,933 - fliT b1926 0.54 +2.3
2,004,035 + fliT b1926 0.81 +0.6
2,004,073 + -1.1
2,004,135 + -2.4
2,004,148 + +0.7
2,004,241 + -0.4
2,004,241 + -0.8
2,004,249 - +0.5
2,004,249 - +1.5
2,004,249 - -0.1
2,004,299 + -1.6
2,004,446 + amyA b1927 0.18 -0.8
2,004,461 - amyA b1927 0.19 +0.1
2,004,533 + amyA b1927 0.24 +0.9
2,004,543 - amyA b1927 0.24 -0.1
2,004,614 - amyA b1927 0.29 +2.0
2,004,614 - amyA b1927 0.29 -0.1
2,004,739 - amyA b1927 0.38 -0.7
2,004,809 + amyA b1927 0.42 +0.5
2,004,861 - amyA b1927 0.46 +0.7
2,004,942 - amyA b1927 0.51 -0.5
2,005,017 - amyA b1927 0.56 +0.9
2,005,185 + amyA b1927 0.68 +1.5
2,005,185 + amyA b1927 0.68 +0.9
2,005,308 + amyA b1927 0.76 +0.9
2,005,308 + amyA b1927 0.76 +0.5
2,005,310 + amyA b1927 0.76 +0.9
2,005,410 - amyA b1927 0.83 +1.0
2,005,410 - amyA b1927 0.83 -0.6
2,005,443 - amyA b1927 0.85 +0.5
2,005,591 - -0.8
2,005,633 + -1.4
2,005,633 + +0.9
2,005,659 - +0.9
2,005,659 - -0.1
2,005,729 + +0.6
2,005,787 - yedD b1928 0.21 +0.6
2,005,788 + yedD b1928 0.21 +0.9
2,005,801 - yedD b1928 0.24 +1.8
2,005,801 - yedD b1928 0.24 -1.6
2,005,809 - yedD b1928 0.26 -0.1
2,005,827 - yedD b1928 0.30 -0.1
2,005,953 + yedD b1928 0.61 +0.9
2,005,969 - yedD b1928 0.65 +1.4
2,006,256 - +1.5
2,006,284 + +0.2
2,006,393 - +0.6
2,006,393 - +0.7
2,006,417 + -3.0
2,006,417 + +0.2
2,006,457 - yedE b1929 0.13 +0.7
2,006,508 + yedE b1929 0.17 -0.5
2,006,605 - yedE b1929 0.25 -0.1

Or see this region's nucleotide sequence