Experiment: Chlorite 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 8 nucleotides ruvA and yobI are separated by 138 nucleotides yobI and yebB are separated by 70 nucleotides yebB and ruvC are separated by 1 nucleotides
b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380
ruvB
b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000
ruvA
b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204
yobI
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400
ruvC
Position (kb)
1943
1944
1945 Strain fitness (log2 ratio)
-2
-1
0
1 at 1942.423 kb on + strand at 1942.445 kb on - strand at 1942.446 kb on - strand at 1942.806 kb on + strand, within ruvB at 1942.985 kb on + strand, within ruvB at 1943.892 kb on + strand, within ruvA at 1943.919 kb on - strand, within ruvA at 1943.934 kb on - strand, within ruvA at 1943.990 kb on - strand at 1943.996 kb on + strand at 1943.996 kb on + strand at 1944.021 kb on + strand at 1944.102 kb on + strand at 1944.102 kb on + strand at 1944.137 kb on + strand at 1944.333 kb on - strand at 1944.333 kb on - strand at 1944.436 kb on - strand, within yebB at 1944.511 kb on - strand, within yebB at 1944.560 kb on - strand, within yebB at 1944.582 kb on + strand, within yebB at 1944.622 kb on + strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.669 kb on - strand, within yebB at 1944.674 kb on - strand, within yebB at 1944.723 kb on - strand, within yebB at 1944.818 kb on + strand at 1944.818 kb on + strand at 1944.826 kb on - strand at 1944.826 kb on - strand at 1944.829 kb on + strand at 1944.846 kb on - strand at 1944.885 kb on - strand at 1944.885 kb on - strand at 1944.895 kb on - strand at 1944.908 kb on + strand at 1944.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 1.25 mM remove 1,942,423 + -1.1 1,942,445 - -0.1 1,942,446 - -0.1 1,942,806 + ruvB b1860 0.43 -2.4 1,942,985 + ruvB b1860 0.61 -0.1 1,943,892 + ruvA b1861 0.82 -1.9 1,943,919 - ruvA b1861 0.87 -1.7 1,943,934 - ruvA b1861 0.89 -1.1 1,943,990 - -0.1 1,943,996 + -0.1 1,943,996 + -1.1 1,944,021 + -1.7 1,944,102 + +0.5 1,944,102 + +0.5 1,944,137 + -0.8 1,944,333 - -0.1 1,944,333 - -0.3 1,944,436 - yebB b1862 0.27 +0.5 1,944,511 - yebB b1862 0.39 -0.4 1,944,560 - yebB b1862 0.47 +0.5 1,944,582 + yebB b1862 0.51 -2.3 1,944,622 + yebB b1862 0.58 -0.7 1,944,625 - yebB b1862 0.58 +0.4 1,944,625 - yebB b1862 0.58 +0.6 1,944,625 - yebB b1862 0.58 -0.1 1,944,669 - yebB b1862 0.65 -0.1 1,944,674 - yebB b1862 0.66 -1.1 1,944,723 - yebB b1862 0.74 +1.1 1,944,818 + +1.3 1,944,818 + -0.1 1,944,826 - -0.5 1,944,826 - -1.7 1,944,829 + +0.2 1,944,846 - +0.8 1,944,885 - -0.1 1,944,885 - -1.1 1,944,895 - -1.1 1,944,908 + -1.1 1,944,916 - -0.4
Or see this region's nucleotide sequence