Strain Fitness in Escherichia coli BW25113 around b1101

Experiment: Chlorite 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfH and ptsG are separated by 294 nucleotidesptsG and fhuE are separated by 59 nucleotides b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797 ycfH b1101: ptsG - fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI), at 1,157,092 to 1,158,525 ptsG b1102: fhuE - ferric-rhodotorulic acid outer membrane transporter (NCBI), at 1,158,585 to 1,160,774 fhuE Position (kb) 1157 1158 1159Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1156.157 kb on + strand, within ycfHat 1156.157 kb on + strand, within ycfHat 1156.258 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.456 kb on + strand, within ycfHat 1156.484 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.617 kb on + strand, within ycfHat 1156.622 kb on + strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.686 kb on + strand, within ycfHat 1156.692 kb on - strand, within ycfHat 1156.696 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.731 kb on + strandat 1157.020 kb on + strandat 1157.020 kb on + strandat 1157.020 kb on - strandat 1157.027 kb on + strandat 1157.072 kb on + strandat 1157.073 kb on + strandat 1157.080 kb on - strandat 1157.080 kb on - strandat 1157.152 kb on + strandat 1157.216 kb on + strandat 1157.216 kb on + strandat 1157.237 kb on - strand, within ptsGat 1157.288 kb on + strand, within ptsGat 1157.313 kb on - strand, within ptsGat 1157.313 kb on - strand, within ptsGat 1157.440 kb on + strand, within ptsGat 1157.440 kb on + strand, within ptsGat 1157.454 kb on + strand, within ptsGat 1157.454 kb on + strand, within ptsGat 1157.509 kb on - strand, within ptsGat 1157.509 kb on - strand, within ptsGat 1157.558 kb on + strand, within ptsGat 1157.625 kb on + strand, within ptsGat 1157.629 kb on - strand, within ptsGat 1157.629 kb on - strand, within ptsGat 1157.644 kb on - strand, within ptsGat 1157.650 kb on + strand, within ptsGat 1157.651 kb on - strand, within ptsGat 1157.671 kb on - strand, within ptsGat 1157.727 kb on - strand, within ptsGat 1157.761 kb on + strand, within ptsGat 1157.901 kb on - strand, within ptsGat 1157.901 kb on - strand, within ptsGat 1157.944 kb on - strand, within ptsGat 1157.945 kb on + strand, within ptsGat 1157.946 kb on - strand, within ptsGat 1157.946 kb on - strand, within ptsGat 1157.965 kb on + strand, within ptsGat 1157.989 kb on + strand, within ptsGat 1157.989 kb on + strand, within ptsGat 1158.000 kb on + strand, within ptsGat 1158.000 kb on + strand, within ptsGat 1158.027 kb on - strand, within ptsGat 1158.027 kb on - strand, within ptsGat 1158.048 kb on - strand, within ptsGat 1158.158 kb on - strand, within ptsGat 1158.265 kb on - strand, within ptsGat 1158.309 kb on - strand, within ptsGat 1158.347 kb on + strand, within ptsGat 1158.353 kb on + strand, within ptsGat 1158.552 kb on - strandat 1158.635 kb on - strandat 1158.686 kb on - strandat 1158.929 kb on + strand, within fhuEat 1159.005 kb on + strand, within fhuEat 1159.053 kb on + strand, within fhuEat 1159.058 kb on + strand, within fhuEat 1159.069 kb on + strand, within fhuEat 1159.077 kb on - strand, within fhuEat 1159.077 kb on - strand, within fhuEat 1159.077 kb on - strand, within fhuEat 1159.084 kb on + strand, within fhuEat 1159.139 kb on - strand, within fhuEat 1159.139 kb on - strand, within fhuEat 1159.245 kb on + strand, within fhuEat 1159.245 kb on + strand, within fhuEat 1159.245 kb on + strand, within fhuEat 1159.388 kb on + strand, within fhuEat 1159.391 kb on - strand, within fhuEat 1159.405 kb on + strand, within fhuEat 1159.410 kb on - strand, within fhuEat 1159.424 kb on - strand, within fhuEat 1159.424 kb on - strand, within fhuEat 1159.442 kb on - strand, within fhuEat 1159.446 kb on + strand, within fhuEat 1159.496 kb on - strand, within fhuEat 1159.496 kb on - strand, within fhuE

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Per-strain Table

Position Strand Gene LocusTag Fraction Chlorite 1.25 mM
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1,156,157 + ycfH b1100 0.20 -0.3
1,156,157 + ycfH b1100 0.20 +0.0
1,156,258 - ycfH b1100 0.32 -0.5
1,156,318 - ycfH b1100 0.40 -3.3
1,156,318 - ycfH b1100 0.40 -0.3
1,156,358 + ycfH b1100 0.45 +0.2
1,156,358 + ycfH b1100 0.45 +0.0
1,156,392 - ycfH b1100 0.49 +0.0
1,156,392 - ycfH b1100 0.49 +0.4
1,156,392 - ycfH b1100 0.49 -0.2
1,156,392 - ycfH b1100 0.49 -1.3
1,156,456 + ycfH b1100 0.57 -0.8
1,156,484 - ycfH b1100 0.61 +0.1
1,156,513 - ycfH b1100 0.64 -0.7
1,156,513 - ycfH b1100 0.64 +0.1
1,156,568 + ycfH b1100 0.71 +0.0
1,156,568 + ycfH b1100 0.71 +0.0
1,156,598 - ycfH b1100 0.75 +0.0
1,156,598 - ycfH b1100 0.75 +0.0
1,156,602 + ycfH b1100 0.75 +1.0
1,156,602 + ycfH b1100 0.75 +1.0
1,156,602 + ycfH b1100 0.75 -1.0
1,156,617 + ycfH b1100 0.77 -0.7
1,156,622 + ycfH b1100 0.78 -0.6
1,156,636 - ycfH b1100 0.80 +0.9
1,156,636 - ycfH b1100 0.80 +1.2
1,156,686 + ycfH b1100 0.86 -0.6
1,156,692 - ycfH b1100 0.87 -0.8
1,156,696 + ycfH b1100 0.87 +0.7
1,156,698 + ycfH b1100 0.87 +0.0
1,156,698 + ycfH b1100 0.87 +0.0
1,156,698 + ycfH b1100 0.87 -2.0
1,156,731 + -0.5
1,157,020 + -0.2
1,157,020 + +1.6
1,157,020 - -1.0
1,157,027 + +0.6
1,157,072 + -0.6
1,157,073 + -0.3
1,157,080 - +1.0
1,157,080 - -1.2
1,157,152 + +1.3
1,157,216 + +0.0
1,157,216 + +2.6
1,157,237 - ptsG b1101 0.10 +0.7
1,157,288 + ptsG b1101 0.14 +0.0
1,157,313 - ptsG b1101 0.15 +0.3
1,157,313 - ptsG b1101 0.15 +1.4
1,157,440 + ptsG b1101 0.24 +0.0
1,157,440 + ptsG b1101 0.24 +1.2
1,157,454 + ptsG b1101 0.25 +0.3
1,157,454 + ptsG b1101 0.25 -2.3
1,157,509 - ptsG b1101 0.29 +3.0
1,157,509 - ptsG b1101 0.29 +0.6
1,157,558 + ptsG b1101 0.32 -1.4
1,157,625 + ptsG b1101 0.37 +0.6
1,157,629 - ptsG b1101 0.37 -1.0
1,157,629 - ptsG b1101 0.37 -1.6
1,157,644 - ptsG b1101 0.38 +1.0
1,157,650 + ptsG b1101 0.39 +0.7
1,157,651 - ptsG b1101 0.39 +0.0
1,157,671 - ptsG b1101 0.40 -0.6
1,157,727 - ptsG b1101 0.44 -1.0
1,157,761 + ptsG b1101 0.47 +1.6
1,157,901 - ptsG b1101 0.56 +1.4
1,157,901 - ptsG b1101 0.56 +2.1
1,157,944 - ptsG b1101 0.59 +2.5
1,157,945 + ptsG b1101 0.59 -0.2
1,157,946 - ptsG b1101 0.60 +1.6
1,157,946 - ptsG b1101 0.60 -0.4
1,157,965 + ptsG b1101 0.61 +1.7
1,157,989 + ptsG b1101 0.63 +0.0
1,157,989 + ptsG b1101 0.63 +0.2
1,158,000 + ptsG b1101 0.63 +1.4
1,158,000 + ptsG b1101 0.63 +0.0
1,158,027 - ptsG b1101 0.65 +0.8
1,158,027 - ptsG b1101 0.65 +1.0
1,158,048 - ptsG b1101 0.67 +0.8
1,158,158 - ptsG b1101 0.74 +1.6
1,158,265 - ptsG b1101 0.82 +0.8
1,158,309 - ptsG b1101 0.85 +1.8
1,158,347 + ptsG b1101 0.88 +1.6
1,158,353 + ptsG b1101 0.88 -1.0
1,158,552 - -0.7
1,158,635 - +0.0
1,158,686 - +0.9
1,158,929 + fhuE b1102 0.16 +0.0
1,159,005 + fhuE b1102 0.19 +1.6
1,159,053 + fhuE b1102 0.21 +0.9
1,159,058 + fhuE b1102 0.22 +1.0
1,159,069 + fhuE b1102 0.22 -2.7
1,159,077 - fhuE b1102 0.22 -2.0
1,159,077 - fhuE b1102 0.22 -1.0
1,159,077 - fhuE b1102 0.22 -1.0
1,159,084 + fhuE b1102 0.23 -1.0
1,159,139 - fhuE b1102 0.25 -1.0
1,159,139 - fhuE b1102 0.25 -1.0
1,159,245 + fhuE b1102 0.30 +0.0
1,159,245 + fhuE b1102 0.30 +1.0
1,159,245 + fhuE b1102 0.30 -1.3
1,159,388 + fhuE b1102 0.37 +1.2
1,159,391 - fhuE b1102 0.37 -0.7
1,159,405 + fhuE b1102 0.37 -1.4
1,159,410 - fhuE b1102 0.38 -0.6
1,159,424 - fhuE b1102 0.38 -2.0
1,159,424 - fhuE b1102 0.38 -1.6
1,159,442 - fhuE b1102 0.39 -1.5
1,159,446 + fhuE b1102 0.39 +0.8
1,159,496 - fhuE b1102 0.42 +0.4
1,159,496 - fhuE b1102 0.42 -1.0

Or see this region's nucleotide sequence